##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544907_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	721966
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.912472332492115	34.0	33.0	34.0	31.0	34.0
2	33.27940789455459	34.0	34.0	34.0	31.0	34.0
3	33.40366582359834	34.0	34.0	34.0	31.0	34.0
4	36.62226476039038	37.0	37.0	37.0	35.0	37.0
5	36.627033683026625	37.0	37.0	37.0	35.0	37.0
6	36.40263945947593	37.0	37.0	37.0	35.0	37.0
7	36.2079114528939	37.0	37.0	37.0	35.0	37.0
8	36.34496499835172	37.0	37.0	37.0	35.0	37.0
9	38.24801998986102	39.0	39.0	39.0	37.0	39.0
10	38.146261735317175	39.0	39.0	39.0	35.0	39.0
11	37.57867683519723	39.0	35.0	39.0	35.0	39.0
12	37.555062149741126	39.0	35.0	39.0	35.0	39.0
13	37.5328076391409	39.0	35.0	39.0	35.0	39.0
14	38.77406830792586	41.0	36.0	41.0	35.0	41.0
15	38.81155068244211	41.0	36.0	41.0	35.0	41.0
16	38.839169711593065	41.0	36.0	41.0	35.0	41.0
17	38.916117933531496	41.0	36.0	41.0	35.0	41.0
18	38.90379463852869	41.0	36.0	41.0	35.0	41.0
19	38.88270500272866	41.0	36.0	41.0	35.0	41.0
20	38.81146203560832	41.0	35.0	41.0	35.0	41.0
21	38.76210652579207	41.0	35.0	41.0	35.0	41.0
22	38.72927395472917	41.0	35.0	41.0	35.0	41.0
23	38.67927575536798	41.0	35.0	41.0	35.0	41.0
24	38.63132058850417	41.0	35.0	41.0	35.0	41.0
25	38.56913344949762	40.0	35.0	41.0	35.0	41.0
26	38.49839189103088	40.0	35.0	41.0	35.0	41.0
27	38.55702623115216	40.0	35.0	41.0	35.0	41.0
28	38.54914774379957	40.0	36.0	41.0	35.0	41.0
29	38.57509494906962	40.0	36.0	41.0	35.0	41.0
30	38.516256998252	40.0	35.0	41.0	35.0	41.0
31	38.41544892695778	40.0	35.0	41.0	35.0	41.0
32	38.376575905236535	40.0	35.0	41.0	35.0	41.0
33	38.28211577830535	40.0	35.0	41.0	35.0	41.0
34	38.16985564417161	40.0	35.0	41.0	35.0	41.0
35	38.102968837867714	40.0	35.0	41.0	35.0	41.0
36	38.080750340043714	40.0	35.0	41.0	35.0	41.0
37	38.02930747431319	40.0	35.0	41.0	35.0	41.0
38	37.97354723075602	40.0	35.0	41.0	35.0	41.0
39	37.91008302330027	40.0	35.0	41.0	35.0	41.0
40	37.847521905463694	40.0	35.0	41.0	35.0	41.0
41	37.797592961441396	40.0	35.0	41.0	35.0	41.0
42	37.73772310607425	40.0	35.0	41.0	35.0	41.0
43	37.6561666338858	40.0	35.0	41.0	35.0	41.0
44	37.52879498480538	39.0	35.0	41.0	35.0	41.0
45	37.51816706049869	39.0	35.0	41.0	35.0	41.0
46	37.45591343636681	39.0	35.0	41.0	35.0	41.0
47	37.36110980295471	39.0	35.0	41.0	35.0	41.0
48	37.299799436538564	39.0	35.0	41.0	35.0	41.0
49	37.23990326414263	39.0	35.0	41.0	35.0	41.0
50	37.17163966170152	39.0	35.0	41.0	35.0	41.0
51	37.07728895820579	38.0	35.0	41.0	34.0	41.0
52	36.61954718089218	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	6.0
15	6.0
16	9.0
17	32.0
18	55.0
19	88.0
20	147.0
21	300.0
22	429.0
23	660.0
24	1129.0
25	1921.0
26	2892.0
27	3514.0
28	3434.0
29	3474.0
30	3885.0
31	4656.0
32	6285.0
33	9523.0
34	34284.0
35	132227.0
36	27042.0
37	44654.0
38	81266.0
39	358265.0
40	1778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.22889720568559	21.53716379995734	22.291908483224972	21.942030511132103
2	28.35299723255666	24.495474856156658	24.611269782787552	22.540258128499126
3	28.512977065401973	24.162633697431733	24.645620430879017	22.678768806287277
4	26.79170487252862	26.776607208649715	21.98025945820163	24.45142846062003
5	25.300083383428028	35.24653515539513	19.967560799262017	19.485820661914826
6	85.13517257045345	3.501272913128873	7.891368845624309	3.472185670793362
7	85.00067870232117	3.2839496596792648	6.515680793832397	5.199690844167177
8	73.40373369383045	6.323150951706867	12.282711374219838	7.990403980242837
9	50.329932434491376	19.81589160708399	15.145588573423124	14.70858738500151
10	39.6478504527914	21.627611272553	24.20737264635731	14.517165628298285
11	38.27063878354382	16.97199037073768	24.514035286980274	20.243335558738224
12	23.77508082098049	28.449816196330573	28.096475457293003	19.678627525395935
13	16.685550289071784	31.64622710764773	29.302903460827796	22.36531914245269
14	15.344905438760273	24.06068983857966	40.293864253995345	20.300540468664728
15	17.907907020552212	17.871755733649508	42.97210671970702	21.248230526091255
16	18.320391819005327	20.92314596532247	28.526689622503	32.22977259316921
17	20.24652130432735	23.827022325151045	30.486477202527546	25.43997916799406
18	25.01862968616251	26.677710584708976	27.12149879634221	21.18216093278631
19	28.184429737688475	22.82614416745387	24.68066363235942	24.308762462498233
20	23.522160323339325	28.406046822149523	26.137103409301822	21.934689445209333
21	21.464307183440773	24.549078488460676	25.437901507827238	28.548712820271316
22	20.838792962549483	28.2928835983966	23.89793979217858	26.97038364687534
23	20.803334229035716	21.97000966804531	33.86669178326957	23.3599643196494
24	22.398838726477425	21.678167669945676	30.39353653773169	25.529457065845207
25	19.272514218121074	27.7855189856586	28.188723568699913	24.75324322752041
26	20.381707725848585	26.75749273511495	26.875503832590454	25.98529570644601
27	22.248277619721705	25.075003532022283	31.983085076028512	20.6936337722275
28	20.01811719665469	24.924857957299928	29.107187873113137	25.949836972932243
29	22.08982140433206	24.063183030779843	27.46237357437885	26.38462199050925
30	26.73076017430184	23.941016612970696	23.496120315915153	25.832102896812316
31	26.575074172467954	23.787546781981423	25.893186105716893	23.744192939833734
32	25.74553372319472	24.68883576234892	25.229996980467224	24.335633533989135
33	20.661914827014012	23.78630018588133	26.852234038722045	28.699550948382612
34	21.819864093322956	20.93242618073427	32.41371477327187	24.833994952670903
35	25.042592033419858	23.84170445699659	26.93908023369522	24.176623275888335
36	21.85144452785865	25.63915752265342	27.47331591792411	25.03608203156381
37	21.71598108498184	26.784086785250278	28.160467390431126	23.339464739336755
38	20.26217301091741	25.989174005424076	26.654025258807202	27.09462772485131
39	24.971674566392323	22.306729125748305	27.829011338484083	24.89258496937529
40	17.65124673461077	21.279533939271378	30.77194770944892	30.29727161666893
41	21.252801378458265	25.273350822614916	24.808924519991248	28.66492327893557
42	22.478205344850036	21.79077685098744	28.32044722327644	27.41057058088608
43	23.093746796940575	24.605175312964878	27.96101201441619	24.340065875678356
44	19.67377965167335	25.753428831828646	28.019602031120577	26.553189485377427
45	21.141161772161016	25.275566993459524	27.280786075798584	26.302485158580875
46	23.65360695656028	22.24467634209921	28.101600352371165	26.000116348969343
47	21.839255588213298	19.556322596908995	31.67545286066103	26.928968954216682
48	20.825634448159608	19.298692736223035	29.19985151655341	30.675821299063944
49	20.082801683181756	22.808414800696987	30.90754966300352	26.20123385311774
50	19.602169631256874	24.125789857140088	28.37183468473585	27.900205826867193
51	20.187238734234022	21.34906629952103	29.70777017200256	28.75592479424239
52	22.273486563079146	19.663668372194813	32.17450683273174	25.888338231994307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	298.5
2	508.0
3	1834.0
4	3160.0
5	2172.5
6	1185.0
7	1198.5
8	1212.0
9	1198.0
10	1184.0
11	1159.0
12	1134.0
13	1124.0
14	1063.5
15	1013.0
16	999.5
17	986.0
18	1050.5
19	1115.0
20	1139.5
21	1164.0
22	1335.5
23	1507.0
24	1594.5
25	1682.0
26	2059.5
27	2437.0
28	3728.0
29	5019.0
30	5416.5
31	5814.0
32	6352.5
33	6891.0
34	8390.0
35	9889.0
36	10658.0
37	11427.0
38	13057.5
39	16426.5
40	18165.0
41	23756.0
42	29347.0
43	38493.0
44	47639.0
45	55011.0
46	62383.0
47	69686.5
48	76990.0
49	77658.0
50	78326.0
51	70936.0
52	63546.0
53	56197.5
54	48849.0
55	43742.0
56	38635.0
57	37072.5
58	35510.0
59	34726.5
60	33943.0
61	34169.5
62	34396.0
63	32030.5
64	23444.0
65	17223.0
66	13890.5
67	10558.0
68	9190.5
69	7823.0
70	6877.5
71	5932.0
72	4821.0
73	3710.0
74	3233.5
75	2757.0
76	2281.5
77	1806.0
78	1281.5
79	757.0
80	624.5
81	492.0
82	356.5
83	221.0
84	125.0
85	29.0
86	35.0
87	41.0
88	22.5
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	721966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.64598376320022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49746953691866	19.051204123906366
2	11.07167093650825	5.9003112801138915
3	3.7837711051872875	3.0246691029745985
4	1.8093908022762641	1.928519917549486
5	1.0812500180754925	1.4405485212799756
6	0.7339960075551236	1.1734827419740568
7	0.5840056503688448	1.0892983554141795
8	0.49957428703457607	1.0649318672668513
9	0.44149796327536	1.0587732804829089
>10	8.07317983662675	51.000995640789405
>50	0.32923286992057577	5.810997634210008
>100	0.08400394937747646	4.129580178694058
>500	0.007826454910945012	1.4705103710078795
>1k	0.0031305819643780053	1.8561769843364089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	3361	0.4655343880459744	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT	2602	0.3604047836047681	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	2187	0.30292285232268557	TruSeq Adapter, Index 23 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2070	0.2867171030214719	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1618	0.2241102766612278	No Hit
TGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTG	1513	0.209566655493472	TruSeq Adapter, Index 23 (96% over 26bp)
GGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCGAAAG	939	0.13006152644307348	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	934	0.12936897305413275	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	876	0.12133535374242001	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	809	0.1120551383306139	TruSeq Adapter, Index 23 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	792	0.10970045680821536	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	776	0.10748428596360493	TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	734	0.10166683749650261	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.385106777881507E-4	0.0	0.0	0.3669147854608112	0.0
2	2.770213555763014E-4	0.0	0.0	1.3452157026785194	0.0
3	2.770213555763014E-4	0.0	0.0	2.0711501649662174	0.0
4	2.770213555763014E-4	0.0	0.0	2.5321137006451826	0.0
5	2.770213555763014E-4	0.0	0.0	4.982644612073145	0.0
6	2.770213555763014E-4	0.0	0.0	5.430449633362236	0.0
7	2.770213555763014E-4	0.0	0.0	7.425695946900547	0.0
8	2.770213555763014E-4	0.0	0.0	9.568040600249873	0.0
9	2.770213555763014E-4	0.0	0.0	11.45566411714679	0.0
10	2.770213555763014E-4	0.0	0.0	12.437704822664779	0.0
11	2.770213555763014E-4	0.0	0.0	13.40755658853741	0.0
12	2.770213555763014E-4	0.0	0.0	14.287514924525532	0.0
13	4.155320333644521E-4	0.0	0.0	14.66509503217603	0.0
14	4.155320333644521E-4	0.0	0.0	14.92951191607361	0.0
15	4.155320333644521E-4	0.0	0.0	15.782045137859678	0.0
16	4.155320333644521E-4	0.0	0.0	16.61324771526637	0.0
17	4.155320333644521E-4	0.0	0.0	17.670361208145536	0.0
18	4.155320333644521E-4	0.0	0.0	18.212907533041722	0.0
19	4.155320333644521E-4	0.0	0.0	18.855043035267588	0.0
20	4.155320333644521E-4	0.0	0.0	19.5948285653341	0.0
21	4.155320333644521E-4	0.0	0.0	20.295692594942143	0.0
22	4.155320333644521E-4	0.0	0.0	21.050298767531988	0.0
23	4.155320333644521E-4	0.0	0.0	21.598108498184125	0.0
24	4.155320333644521E-4	0.0	0.0	22.069875866730566	0.0
25	4.155320333644521E-4	0.0	0.0	22.519343016153115	0.0
26	4.155320333644521E-4	0.0	0.0	22.956621225930306	0.0
27	4.155320333644521E-4	0.0	0.0	23.38115645335099	0.0
28	5.540427111526028E-4	0.0	0.0	23.802505935182545	0.0
29	5.540427111526028E-4	0.0	0.0	24.29851267234191	0.0
30	5.540427111526028E-4	0.0	0.0	24.825268779970248	0.0
31	5.540427111526028E-4	0.0	0.0	25.317674239507124	0.0
32	5.540427111526028E-4	0.0	0.0	25.73902372133868	0.0
33	5.540427111526028E-4	0.0	0.0	26.16937639722646	0.0
34	5.540427111526028E-4	0.0	0.0	26.698625697054986	0.0
35	5.540427111526028E-4	0.0	0.0	27.1508630600333	0.0
36	5.540427111526028E-4	0.0	0.0	27.621660853835223	0.0
37	5.540427111526028E-4	0.0	0.0	28.041486718211107	0.0
38	5.540427111526028E-4	0.0	0.0	28.474471096976867	0.0
39	6.925533889407535E-4	0.0	0.0	28.93100229096661	0.0
40	6.925533889407535E-4	0.0	0.0	29.417728812714174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	35	1.0188705E-7	46.000004	1
ACGGGTA	35	1.0188705E-7	46.000004	4
GCCCATT	20	6.309888E-4	46.0	35
ACCGGTA	30	1.8604351E-6	46.0	41
CGAACTA	20	6.309888E-4	46.0	22
GCCGATG	25	3.415884E-5	46.0	8
TACGCGG	65	0.0	46.0	1
AACGATC	20	6.309888E-4	46.0	46
CTACCGG	20	6.309888E-4	46.0	1
ACCGTTG	20	6.309888E-4	46.0	35
CCGGTAT	30	1.8604351E-6	46.0	42
TCGACAA	85	0.0	46.0	18
TGGCTAC	20	6.309888E-4	46.0	32
TCGGTAC	20	6.309888E-4	46.0	35
ATACCGG	40	5.6061253E-9	46.0	1
TACCGGT	30	1.8604351E-6	46.0	40
GTTGCAT	20	6.309888E-4	46.0	44
TATCCGG	50	1.6370905E-11	46.0	1
CTAGCGG	150	0.0	44.466663	1
TACGGGA	125	0.0	44.16	3
>>END_MODULE