FastQCFastQC Report
Thu 26 May 2016
SRR1544906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544906_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1055738
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT26060.2468415459138536No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT20550.19465056671257452Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.1817685827354893No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16480.1560993352517386No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT15670.1484269771477393No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13580.12863039883001273No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA12110.11470648967831035No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11760.11139127321361929No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG10570.10011953723366972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCA206.311218E-446.00000414
TAACGAT206.311218E-446.00000430
ACGGGTC405.6097633E-946.0000044
ACGATAG206.311218E-446.00000432
CGGTATT206.311218E-446.00000443
CGCAAAC206.311218E-446.00000423
CCTAACG253.4169654E-546.036
AATACGG301.8612591E-646.01
ATCGATT253.4169654E-546.012
CTAACGG900.045.9999961
CACGACC2100.044.90476226
ACGTAGG1600.044.5625041
TAACGGG3150.042.349212
ACACGAC2250.041.9111125
CGTAGGG4450.041.865172
GCGGGAT3150.041.619054
GCGATGA1050.041.6190458
CGAATAT1500.041.39999814
TACGTGA508.712959E-1041.39999824
GCGAGAC2450.041.3061220