##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544906_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1055738
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.958664933913525	34.0	33.0	34.0	31.0	34.0
2	33.2956623707776	34.0	33.0	34.0	31.0	34.0
3	33.368637862803084	34.0	34.0	34.0	31.0	34.0
4	36.63268538216868	37.0	37.0	37.0	35.0	37.0
5	36.63195887616056	37.0	37.0	37.0	35.0	37.0
6	36.68522209108699	37.0	37.0	37.0	36.0	37.0
7	36.50913578937198	37.0	37.0	37.0	35.0	37.0
8	36.54530574820647	37.0	37.0	37.0	35.0	37.0
9	38.35264525857741	39.0	39.0	39.0	37.0	39.0
10	38.17638467119683	39.0	39.0	39.0	35.0	39.0
11	37.62698984028234	39.0	35.0	39.0	35.0	39.0
12	37.58089791217139	39.0	35.0	39.0	35.0	39.0
13	37.57840013336642	39.0	35.0	39.0	35.0	39.0
14	38.913049449768785	41.0	37.0	41.0	35.0	41.0
15	38.96662713665701	41.0	37.0	41.0	35.0	41.0
16	38.957493241694436	41.0	36.0	41.0	35.0	41.0
17	39.00479096139383	41.0	36.0	41.0	35.0	41.0
18	38.97829575140802	41.0	36.0	41.0	35.0	41.0
19	38.91021162447501	41.0	36.0	41.0	35.0	41.0
20	38.837390526816314	41.0	35.0	41.0	35.0	41.0
21	38.781558492732096	41.0	35.0	41.0	35.0	41.0
22	38.737923613623835	41.0	35.0	41.0	35.0	41.0
23	38.70876960003334	41.0	35.0	41.0	35.0	41.0
24	38.64909665087361	41.0	35.0	41.0	35.0	41.0
25	38.58070373520703	40.0	35.0	41.0	35.0	41.0
26	38.528727771473605	40.0	35.0	41.0	35.0	41.0
27	38.487652239476084	40.0	36.0	41.0	35.0	41.0
28	38.50573911330273	40.0	36.0	41.0	35.0	41.0
29	38.5025044092379	40.0	36.0	41.0	35.0	41.0
30	38.42538679104096	40.0	35.0	41.0	35.0	41.0
31	38.35872915439247	40.0	35.0	41.0	35.0	41.0
32	38.25336020868814	40.0	35.0	41.0	35.0	41.0
33	38.155531959633926	40.0	35.0	41.0	35.0	41.0
34	38.03200983577365	40.0	35.0	41.0	35.0	41.0
35	37.931673388662716	40.0	35.0	41.0	35.0	41.0
36	37.87407481780517	40.0	35.0	41.0	35.0	41.0
37	37.83385271724614	40.0	35.0	41.0	35.0	41.0
38	37.75303152865578	40.0	35.0	41.0	35.0	41.0
39	37.6537891029782	40.0	35.0	41.0	35.0	41.0
40	37.583391902157544	40.0	35.0	41.0	34.0	41.0
41	37.51670963818675	39.0	35.0	41.0	34.0	41.0
42	37.42883556336894	39.0	35.0	41.0	34.0	41.0
43	37.31920419649572	39.0	35.0	41.0	34.0	41.0
44	37.223994021243904	39.0	35.0	41.0	34.0	41.0
45	37.17520066531658	39.0	35.0	41.0	34.0	41.0
46	37.15765085655721	39.0	35.0	41.0	34.0	41.0
47	37.105744985971896	38.0	35.0	41.0	34.0	41.0
48	37.07848443458509	38.0	35.0	41.0	34.0	41.0
49	37.00586224991428	38.0	35.0	41.0	34.0	41.0
50	36.9313323949692	38.0	35.0	41.0	34.0	41.0
51	36.84486965516066	37.0	35.0	41.0	34.0	41.0
52	36.62951698243314	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	2.0
13	5.0
14	10.0
15	14.0
16	24.0
17	42.0
18	87.0
19	128.0
20	286.0
21	487.0
22	760.0
23	1177.0
24	1921.0
25	3059.0
26	4384.0
27	5100.0
28	5288.0
29	5565.0
30	6336.0
31	7832.0
32	10313.0
33	15389.0
34	51510.0
35	184899.0
36	44837.0
37	75539.0
38	125950.0
39	500726.0
40	4066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.40431053916787	22.129354063224017	23.747653300345352	20.718682097262768
2	28.061697125612607	24.38891088508702	25.33384229799439	22.215549691305984
3	28.013200244757698	25.28203020067479	23.986917208625623	22.717852345941893
4	26.698006512979543	26.475223966552306	21.663802951110977	25.16296656935717
5	24.998342391767654	36.54230500370357	19.36427409073084	19.09507851379793
6	88.33583711110144	3.490259894026738	5.711076043488062	2.4628269513837715
7	87.804076390165	3.308396590820829	4.948007933786602	3.939519085227585
8	78.25937874737862	6.0279160170421076	9.47223648291527	6.240468752664014
9	51.780839564361614	20.259856138549527	14.032174649392179	13.927129647696681
10	39.5172855386469	22.897631798798564	21.731054485108995	15.854028177445539
11	37.08060143709898	18.630190444977828	25.075634295630167	19.213573822293032
12	22.737648924259616	29.316459197262958	27.70479039307101	20.241101485406418
13	17.469391079983858	31.26362790768164	29.16575892882514	22.101222083509356
14	15.809509556348262	23.294605290327713	39.83118917761793	21.064695975706094
15	18.69990471120676	18.626496346631455	40.97399165323215	21.69960728892964
16	19.524351685740214	20.349745864977862	28.369159772595093	31.756742676686827
17	21.53223621769795	22.420051186942214	30.840795727727905	25.20691686763193
18	25.460294126004747	25.51021181391595	25.954734981595813	23.07475907848349
19	28.410647338638945	22.642265410546937	25.189393580604282	23.757693670209843
20	24.723937188961656	27.690392881567206	25.551604659489378	22.034065269981756
21	23.11406807370768	23.591553965093613	24.19662833013494	29.097749631063767
22	22.09345500493494	27.74874069134577	23.558970123269223	26.598834180450076
23	22.693319744103178	21.25631548736524	33.20141929152877	22.848945477002815
24	21.437989349630307	21.865273391693773	31.20139655861587	25.495340700060055
25	19.726864051497625	28.04900458257636	27.669649098545285	24.554482267380735
26	20.996023634651777	27.1669675620277	26.936228496085203	24.90078030723532
27	22.074889792732666	25.37144632475103	32.15987299879326	20.393790883723046
28	21.35937135918192	24.194733920726545	28.94847016968225	25.497424550409285
29	22.37799529807585	25.74417137585272	28.539846060291474	23.337987265779958
30	28.743400351223503	23.61760209445904	23.179046316415626	24.459951237901826
31	30.28535488918652	23.241845988303915	24.620976037615392	21.851823084894168
32	27.92615213244195	24.10370754865317	25.36263732100199	22.60750299790289
33	24.928817566479562	23.750400193987524	26.382682066952217	24.938100172580697
34	26.076356065614764	22.574824435608075	28.065770105840652	23.283049392936505
35	30.36833002127422	22.16979970409325	24.705371976759384	22.756498297873147
36	26.44908111671646	26.015829685016545	26.497293836160107	21.037795362106888
37	26.81877511276472	25.37438265933404	25.016907603969923	22.789934623931316
38	24.557323881493325	24.877100189630383	25.242815925921015	25.32276000295528
39	27.38984482892536	21.86176873428824	27.22323152145703	23.52515491532937
40	20.244890304223205	21.059486349833005	30.60172126038847	28.093902085555317
41	23.31904317169601	24.03996067206068	24.939426259166574	27.701569897076737
42	24.505606504644145	21.812798251081233	26.73276892562359	26.94882631865103
43	24.09698239525337	25.742371686914744	27.758212738387744	22.40243317944414
44	21.708984615501194	27.217074690879745	26.80769281772561	24.266247875893452
45	24.664831615419736	25.864182211874535	25.516842246845332	23.954143925860393
46	24.719864208733604	22.475178500726507	27.88968475133035	24.91527253920954
47	24.883446461148502	20.319435314443545	30.22255521729823	24.574563007109717
48	23.4845198335193	20.604638650877398	28.600561881830526	27.310279633772772
49	22.34001238943753	25.355817447131766	28.305791777884288	23.998378385546417
50	22.201531061683866	25.64338879532611	26.358623067465604	25.79645707552442
51	22.54063034578655	22.235062108212457	29.562732420354294	25.661575125646703
52	25.235048847346597	20.721050109023263	29.39592967194512	24.64797137168502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	398.5
2	644.0
3	2269.0
4	3894.0
5	2677.0
6	1460.0
7	1569.5
8	1679.0
9	1658.0
10	1637.0
11	1594.0
12	1551.0
13	1485.0
14	1470.0
15	1521.0
16	1371.0
17	1221.0
18	1317.0
19	1413.0
20	1614.0
21	1815.0
22	1824.5
23	1834.0
24	2659.0
25	3484.0
26	3776.5
27	4069.0
28	5884.0
29	7699.0
30	8540.5
31	9382.0
32	10440.5
33	11499.0
34	13851.0
35	16203.0
36	17034.0
37	17865.0
38	20911.5
39	27634.5
40	31311.0
41	39473.5
42	47636.0
43	58961.5
44	70287.0
45	74013.5
46	77740.0
47	76716.5
48	75693.0
49	82894.0
50	90095.0
51	92914.0
52	95733.0
53	88011.0
54	80289.0
55	73139.0
56	65989.0
57	59515.5
58	53042.0
59	53336.5
60	53631.0
61	52819.5
62	52008.0
63	48527.5
64	37738.0
65	30429.0
66	26169.0
67	21909.0
68	18865.0
69	15821.0
70	13348.0
71	10875.0
72	10164.5
73	9454.0
74	8104.5
75	6755.0
76	5328.5
77	3902.0
78	3025.0
79	2148.0
80	1483.5
81	819.0
82	619.5
83	420.0
84	323.5
85	227.0
86	132.5
87	38.0
88	33.0
89	16.5
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1055738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.118014661430006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74065910196599	15.361383053973999
2	10.326221982865412	4.361386144626652
3	3.4387830845076794	2.1786081478833204
4	1.6530446439387503	1.3963608410678747
5	0.9219592890300988	0.9734974891489606
6	0.6699814083816891	0.848920632305402
7	0.48734899312433777	0.720429022742304
8	0.40264962101647	0.6802528480036039
9	0.3490271408145461	0.6633684249262722
>10	6.930061970166687	39.893409544368154
>50	1.9182051772875388	25.58228216950285
>100	0.15128367530636608	5.020119138784636
>500	0.006733694746574157	0.9361649071930027
>1k	0.004040216847944494	1.3838176354730392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	2606	0.2468415459138536	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT	2055	0.19465056671257452	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1919	0.1817685827354893	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1648	0.1560993352517386	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	1567	0.1484269771477393	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1358	0.12863039883001273	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1211	0.11470648967831035	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1176	0.11139127321361929	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1057	0.10011953723366972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19862882647020377	0.0
2	0.0	0.0	0.0	0.7007420401652683	0.0
3	0.0	0.0	0.0	1.1080400629701688	0.0
4	0.0	0.0	0.0	1.3637853331034784	0.0
5	0.0	0.0	0.0	2.757123452977917	0.0
6	0.0	0.0	0.0	3.0639230566674684	0.0
7	0.0	0.0	0.0	4.513430415500816	0.0
8	9.47204704197443E-5	0.0	0.0	6.04553402454018	0.0
9	9.47204704197443E-5	0.0	0.0	7.663643820720671	0.0
10	9.47204704197443E-5	0.0	0.0	8.304333082639822	0.0
11	9.47204704197443E-5	0.0	0.0	8.929298746469295	0.0
12	9.47204704197443E-5	0.0	0.0	9.458786176115664	0.0
13	9.47204704197443E-5	0.0	0.0	9.663477112692732	0.0
14	9.47204704197443E-5	0.0	0.0	9.811714648899633	0.0
15	9.47204704197443E-5	0.0	0.0	10.311175689422944	0.0
16	9.47204704197443E-5	0.0	0.0	10.851366532226745	0.0
17	9.47204704197443E-5	0.0	0.0	11.550498324394878	9.47204704197443E-5
18	1.894409408394886E-4	0.0	0.0	11.885714069210353	9.47204704197443E-5
19	1.894409408394886E-4	0.0	0.0	12.322091276434115	9.47204704197443E-5
20	2.841614112592329E-4	0.0	0.0	12.800334931583404	9.47204704197443E-5
21	2.841614112592329E-4	0.0	0.0	13.240879839505634	9.47204704197443E-5
22	2.841614112592329E-4	0.0	0.0	13.714198030193097	9.47204704197443E-5
23	3.788818816789772E-4	0.0	0.0	14.064095447923632	9.47204704197443E-5
24	3.788818816789772E-4	0.0	0.0	14.385103122176146	9.47204704197443E-5
25	3.788818816789772E-4	0.0	0.0	14.689345273164365	9.47204704197443E-5
26	3.788818816789772E-4	0.0	0.0	14.971706995485622	9.47204704197443E-5
27	3.788818816789772E-4	0.0	0.0	15.273486414242928	9.47204704197443E-5
28	3.788818816789772E-4	0.0	0.0	15.56920372289337	9.47204704197443E-5
29	4.736023520987215E-4	0.0	0.0	15.958978458670616	9.47204704197443E-5
30	0.004830743991406959	0.0	0.0	16.318347923443127	9.47204704197443E-5
31	0.004830743991406959	0.0	0.0	16.65801553036833	9.47204704197443E-5
32	0.004830743991406959	0.0	0.0	16.977602397564546	9.47204704197443E-5
33	0.004830743991406959	0.0	0.0	17.281939269023187	9.47204704197443E-5
34	0.004830743991406959	0.0	0.0	17.649359973781372	9.47204704197443E-5
35	0.004830743991406959	0.0	0.0	17.974251187321098	9.47204704197443E-5
36	0.004830743991406959	0.0	0.0	18.316760408358892	9.47204704197443E-5
37	0.004830743991406959	0.0	0.0	18.637862803081827	9.47204704197443E-5
38	0.004830743991406959	0.0	0.0	18.968058362965053	9.47204704197443E-5
39	0.004830743991406959	0.0	0.0	19.324017890802452	9.47204704197443E-5
40	0.004830743991406959	0.0	0.0	19.682155989459506	9.47204704197443E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	20	6.311218E-4	46.000004	14
TAACGAT	20	6.311218E-4	46.000004	30
ACGGGTC	40	5.6097633E-9	46.000004	4
ACGATAG	20	6.311218E-4	46.000004	32
CGGTATT	20	6.311218E-4	46.000004	43
CGCAAAC	20	6.311218E-4	46.000004	23
CCTAACG	25	3.4169654E-5	46.0	36
AATACGG	30	1.8612591E-6	46.0	1
ATCGATT	25	3.4169654E-5	46.0	12
CTAACGG	90	0.0	45.999996	1
CACGACC	210	0.0	44.904762	26
ACGTAGG	160	0.0	44.562504	1
TAACGGG	315	0.0	42.34921	2
ACACGAC	225	0.0	41.91111	25
CGTAGGG	445	0.0	41.86517	2
GCGGGAT	315	0.0	41.61905	4
GCGATGA	105	0.0	41.619045	8
CGAATAT	150	0.0	41.399998	14
TACGTGA	50	8.712959E-10	41.399998	24
GCGAGAC	245	0.0	41.30612	20
>>END_MODULE