FastQCFastQC Report
Thu 26 May 2016
SRR1544905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544905_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences631638
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18920.2995386598019752No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT18770.2971638818437143No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT15520.24571035941472805Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.19963966702446653No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT12170.19267365168023456No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10240.1621181752839443No Hit
TGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTTG8970.14201172190400196Illumina Single End Adapter 1 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG8020.12697146150168293No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA6990.11066465285495806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA206.3092844E-446.08
CCGATAG253.4153956E-546.011
CTAACGG501.6370905E-1146.01
TCTCGAG206.3092844E-446.01
CGCAAAC206.3092844E-446.023
ATACCGG301.8600622E-646.01
TAGTCGG206.3092844E-446.01
AGTCGTG206.3092844E-446.018
ATTTCGG301.8600622E-646.01
CTAGCTA206.3092844E-446.023
CTATCGG351.0185977E-745.9999961
CCGTAGG453.092282E-1045.9999961
AAGTAGG750.042.933331
CGTTTTT11200.042.0982131
GCGGGAT1750.042.0571444
TCGGGCA554.7293724E-1141.818184
ATAGCGG1050.041.6190451
TTATGAC3650.041.58904326
CGCACTT3500.041.434
AATGCGG508.712959E-1041.3999981
Produced by FastQC (version 0.11.5)