FastQCFastQC Report
Thu 26 May 2016
SRR1544904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544904_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1124313
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT30590.27207725962432167No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT27590.2453942985627668Illumina Single End Adapter 1 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT24680.2195118263330585No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.200655867182893No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT22020.19585293419181315No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA16120.14337644410408845No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14340.12754455387423252No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11850.10539769619314193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT206.3113956E-446.010
CAACGTA206.3113956E-446.010
CGACCGA301.8613664E-646.020
CGTTCCG206.3113956E-446.035
AAAGTCG206.3113956E-446.013
TACGATG206.3113956E-446.028
ACTCGTA206.3113956E-446.015
TCGTATC206.3113956E-446.024
TGCTCGA206.3113956E-446.030
ACAGACG206.3113956E-446.015
CGTAATA206.3113956E-446.013
ATACGGT206.3113956E-446.030
TGTAACG206.3113956E-446.021
AGTACGG1050.043.8095251
ACGGGAT4000.043.6999974
CTCGAGG1700.043.2941171
TTACGGG3300.043.212122
CGGGATA1600.043.1255
ACTACGG750.042.9333341
CGAATAT1450.042.82758314
Produced by FastQC (version 0.11.5)