##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544904_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1124313
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94490057483993	34.0	33.0	34.0	31.0	34.0
2	33.25914669669389	34.0	33.0	34.0	31.0	34.0
3	33.313621740565125	34.0	34.0	34.0	31.0	34.0
4	36.589459518835056	37.0	37.0	37.0	35.0	37.0
5	36.58458454184911	37.0	37.0	37.0	35.0	37.0
6	36.66829788501956	37.0	37.0	37.0	35.0	37.0
7	36.48278726653521	37.0	37.0	37.0	35.0	37.0
8	36.51394940732696	37.0	37.0	37.0	35.0	37.0
9	38.32606222644406	39.0	39.0	39.0	37.0	39.0
10	38.12650213952876	39.0	39.0	39.0	35.0	39.0
11	37.48797087643743	39.0	35.0	39.0	35.0	39.0
12	37.42039538811701	39.0	35.0	39.0	35.0	39.0
13	37.419638481454896	39.0	35.0	39.0	35.0	39.0
14	38.72307444635079	41.0	35.0	41.0	35.0	41.0
15	38.771404404289555	41.0	35.0	41.0	35.0	41.0
16	38.76285874129357	41.0	35.0	41.0	35.0	41.0
17	38.805108541838436	41.0	36.0	41.0	35.0	41.0
18	38.7898476669753	41.0	36.0	41.0	35.0	41.0
19	38.72464607275732	41.0	36.0	41.0	35.0	41.0
20	38.632953634797424	41.0	35.0	41.0	35.0	41.0
21	38.581586266457826	41.0	35.0	41.0	35.0	41.0
22	38.55739549395942	41.0	35.0	41.0	35.0	41.0
23	38.53471141932896	40.0	35.0	41.0	35.0	41.0
24	38.486418817535686	40.0	35.0	41.0	35.0	41.0
25	38.42659206110754	40.0	35.0	41.0	35.0	41.0
26	38.37485202074511	40.0	35.0	41.0	35.0	41.0
27	38.348289133008336	40.0	35.0	41.0	35.0	41.0
28	38.396056080468696	40.0	35.0	41.0	35.0	41.0
29	38.40162214614614	40.0	36.0	41.0	35.0	41.0
30	38.33450916248411	40.0	35.0	41.0	35.0	41.0
31	38.26348801445861	40.0	35.0	41.0	35.0	41.0
32	38.18734729563742	40.0	35.0	41.0	35.0	41.0
33	38.11240375233587	40.0	35.0	41.0	35.0	41.0
34	38.02497258325751	40.0	35.0	41.0	35.0	41.0
35	37.944443406773736	40.0	35.0	41.0	35.0	41.0
36	37.905866960535015	40.0	35.0	41.0	35.0	41.0
37	37.82735679477156	40.0	35.0	41.0	35.0	41.0
38	37.76508321081407	40.0	35.0	41.0	35.0	41.0
39	37.6344585538013	40.0	35.0	41.0	35.0	41.0
40	37.548215665922214	40.0	35.0	41.0	34.0	41.0
41	37.49841369796489	39.0	35.0	41.0	35.0	41.0
42	37.422152016386896	39.0	35.0	41.0	35.0	41.0
43	37.32963151720206	39.0	35.0	41.0	34.0	41.0
44	37.263388398070646	39.0	35.0	41.0	34.0	41.0
45	37.22399011663122	39.0	35.0	41.0	34.0	41.0
46	37.22671622581968	39.0	35.0	41.0	34.0	41.0
47	37.16739822451577	39.0	35.0	41.0	34.0	41.0
48	37.15698386481345	39.0	35.0	41.0	34.0	41.0
49	37.08278477612551	38.0	35.0	41.0	34.0	41.0
50	37.018649610917954	38.0	35.0	41.0	34.0	41.0
51	36.93298930102205	38.0	35.0	41.0	34.0	41.0
52	36.72237713163505	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	10.0
14	11.0
15	19.0
16	31.0
17	56.0
18	106.0
19	191.0
20	294.0
21	517.0
22	831.0
23	1277.0
24	2097.0
25	3366.0
26	4556.0
27	5610.0
28	5859.0
29	6365.0
30	7456.0
31	9232.0
32	11916.0
33	16387.0
34	53687.0
35	224895.0
36	42509.0
37	65941.0
38	115038.0
39	540329.0
40	5722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.80153035676008	22.098917294383323	23.858658576392873	20.240893772463718
2	28.068340399870856	23.77985489805775	25.270987705380975	22.880816996690424
3	27.58359994058594	24.793629531989758	24.655233907283826	22.96753662014048
4	26.134181495722274	27.278168979634675	22.100962988064712	24.486686536578336
5	24.78019910825544	36.34237085224488	20.02200454855543	18.855425490944246
6	87.84528863403696	3.3266536987475908	6.372157931109931	2.455899736105515
7	86.87438462421052	3.263237194624628	5.168667444030266	4.693710737134587
8	76.28969868710938	6.073219824016977	10.618217524835167	7.018863964038484
9	50.37876463226877	21.276281604855587	14.192578045437527	14.152375717438115
10	40.10964918132228	21.27903884416528	24.533381718436058	14.077930256076376
11	39.51862159380884	17.505356604433107	24.58247836678932	18.39354343496873
12	21.615244153540875	31.882402854009516	27.465038650269097	19.037314342180515
13	16.08964763371054	33.82661234015794	28.489308582218655	21.59443144391286
14	14.533764174211274	23.78101115970375	41.96349237267558	19.721732293409396
15	17.034491284900202	18.925868508146753	43.154886584074006	20.88475362287904
16	18.337776046350083	21.867398135572564	27.414252081048605	32.38057373702875
17	20.184059065402607	23.96085431725863	32.41401638155923	23.441070235779538
18	24.8463728516881	26.563332452795617	26.28307241844575	22.307222277070533
19	27.525786858285905	23.90900042959567	24.87083223266119	23.694380479457234
20	21.86232837297087	30.537136900489454	26.118171719085343	21.48236300745433
21	22.04234941693283	24.220746357998173	24.748090611778036	28.988813613290958
22	19.826685273584847	29.217664475995562	24.03280936892129	26.9228408814983
23	19.655202777162586	22.79160696354129	35.397971917072915	22.155218342223208
24	19.895260483513045	23.058347630953303	32.72745223082896	24.318939654704696
25	18.028698414053736	29.596384636662563	28.185745428541697	24.189171520742
26	19.431510620263218	29.04413628589192	27.086852148823326	24.437500945021537
27	19.10846890501133	27.557450638745618	33.776804146176374	19.557276310066683
28	18.3546752550224	24.617344102576418	30.313711573200703	26.71426906920048
29	19.663652381498746	29.176928488774923	28.301015820327613	22.858403309398717
30	26.516726214141435	24.166579947043218	23.956140327471086	25.360553511344257
31	27.726620611875873	25.49841547682896	25.16719098685153	21.607772924443637
32	24.332636908049626	26.763009944739586	24.732525551158798	24.17182759605199
33	18.900964411155968	25.141664287435972	28.56980218142101	27.38756911998705
34	21.818924089644078	22.281339804840822	30.762074262238364	25.13766184327674
35	26.289654215507603	22.770349537895587	28.82836007410748	22.111636172489334
36	24.000611929240346	26.04452674655545	29.2348305142785	20.720030809925703
37	22.06111643287946	26.383133522426583	27.927632251872918	23.628117792821037
38	23.941731528497847	26.044348860148375	25.964655749777865	24.049263861575916
39	26.967668256081712	22.12319878894934	26.849462738579028	24.059670216389918
40	19.661962460631514	20.961689493939854	29.838754866305024	29.537593179123604
41	22.214454515779856	24.641091937921203	24.57767543379824	28.5667781125007
42	22.309267970751918	22.592996790039784	27.730534112831567	27.367201126376727
43	21.974663639040017	26.997108456452963	28.729544174976184	22.298683729530833
44	18.626040969018415	29.452652419744325	27.519649777241746	24.401656833995517
45	22.251721718062498	27.14253059423844	26.540562992689757	24.065184695009307
46	23.72933515844787	22.921552983911063	28.67413255917169	24.674979298469378
47	22.941920977521384	20.49135783362818	31.18437659263924	25.382344596211198
48	22.009618318030654	19.875959808345183	29.65313040052014	28.461291473104023
49	20.809329786278376	24.70353006680524	30.45486443721633	24.03227570970006
50	20.6124095336441	25.081894454658087	27.4599688876674	26.845727124030404
51	20.583947708511776	21.703653697858158	31.0595892780747	26.652809315555366
52	23.644572285475665	19.537708805288208	31.81622911057686	25.00148979865927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	140.0
1	406.5
2	673.0
3	2509.5
4	4346.0
5	2952.0
6	1558.0
7	1682.0
8	1806.0
9	1765.5
10	1725.0
11	1675.0
12	1625.0
13	1528.5
14	1442.0
15	1452.0
16	1346.0
17	1240.0
18	1441.0
19	1642.0
20	1639.0
21	1636.0
22	2049.5
23	2463.0
24	2837.5
25	3212.0
26	4142.5
27	5073.0
28	8357.0
29	11641.0
30	11078.0
31	10515.0
32	12363.0
33	14211.0
34	17142.5
35	20074.0
36	20841.0
37	21608.0
38	24665.0
39	32713.0
40	37704.0
41	47278.0
42	56852.0
43	72455.5
44	88059.0
45	99605.0
46	111151.0
47	113580.5
48	116010.0
49	115649.5
50	115289.0
51	103730.0
52	92171.0
53	79493.5
54	66816.0
55	61021.0
56	55226.0
57	50272.5
58	45319.0
59	45734.0
60	46149.0
61	45072.0
62	43995.0
63	40418.5
64	31195.5
65	25549.0
66	20673.5
67	15798.0
68	13334.0
69	10870.0
70	8787.5
71	6705.0
72	6903.5
73	7102.0
74	5745.5
75	4389.0
76	3630.5
77	2872.0
78	2445.5
79	2019.0
80	1561.5
81	1104.0
82	714.0
83	324.0
84	217.0
85	110.0
86	68.5
87	27.0
88	16.5
89	7.0
90	8.0
91	28.5
92	49.0
93	26.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1124313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.90350859270195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84791640955888	14.809700294422434
2	11.784567618405854	4.926776209452479
3	4.285883130638595	2.687699845458606
4	2.1294698120772653	1.780535620586261
5	1.1365185073836632	1.18786121924296
6	0.7397283425620311	0.9277750659006354
7	0.5312821056301068	0.7773962042131407
8	0.38773672626512495	0.6484046391351331
9	0.3290825488075427	0.6191081898036055
>10	5.307887477223688	29.31780094057604
>50	2.261068651210442	31.245853076517538
>100	0.24649145205418804	8.198237766186267
>500	0.008102660188632479	1.1752774580758079
>1k	0.004264557994017094	1.6975734704291268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	3059	0.27207725962432167	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	2759	0.2453942985627668	Illumina Single End Adapter 1 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2468	0.2195118263330585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	0.200655867182893	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2202	0.19585293419181315	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1612	0.14337644410408845	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1434	0.12754455387423252	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1185	0.10539769619314193	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24868519709369188	0.0
2	0.0	0.0	0.0	0.8708429058456142	0.0
3	0.0	0.0	0.0	1.4380337148107334	0.0
4	0.0	0.0	0.0	1.7999436100089565	0.0
5	0.0	0.0	0.0	3.685272695414889	0.0
6	0.0	0.0	0.0	4.099303307886683	0.0
7	0.0	0.0	0.0	6.108352389414692	0.0
8	0.0	0.0	0.0	8.21506110842799	0.0
9	0.0	0.0	0.0	10.283079533902036	0.0
10	0.0	0.0	0.0	11.10838351953593	0.0
11	0.0	0.0	0.0	11.925060014426588	0.0
12	0.0	0.0	0.0	12.603785600629006	0.0
13	0.0	0.0	0.0	12.864744959811013	0.0
14	0.0	0.0	0.0	13.069492214356679	0.0
15	0.0	0.0	0.0	13.720022805037388	0.0
16	0.0	0.0	0.0	14.416003372726278	0.0
17	0.0	0.0	0.0	15.415547094092126	0.0
18	0.0	0.0	0.0	15.861241487023632	0.0
19	0.0	0.0	0.0	16.4481776871743	0.0
20	8.894320353851641E-5	0.0	0.0	17.078251341041153	0.0
21	8.894320353851641E-5	0.0	0.0	17.615290404006714	0.0
22	8.894320353851641E-5	0.0	0.0	18.202582376971538	0.0
23	8.894320353851641E-5	0.0	0.0	18.64000505197396	0.0
24	8.894320353851641E-5	0.0	0.0	19.018903099048043	0.0
25	8.894320353851641E-5	0.0	0.0	19.375387458830414	0.0
26	8.894320353851641E-5	0.0	0.0	19.710614392967084	0.0
27	8.894320353851641E-5	0.0	0.0	20.07288006097946	0.0
28	1.7788640707703282E-4	0.0	0.0	20.419313838761983	0.0
29	1.7788640707703282E-4	0.0	0.0	20.879150201056113	0.0
30	1.7788640707703282E-4	0.0	0.0	21.299140008164986	0.0
31	1.7788640707703282E-4	0.0	0.0	21.677949112035527	0.0
32	1.7788640707703282E-4	0.0	0.0	22.054445692614067	0.0
33	1.7788640707703282E-4	0.0	0.0	22.41831233829014	0.0
34	1.7788640707703282E-4	0.0	0.0	22.89193489713274	0.0
35	1.7788640707703282E-4	0.0	0.0	23.2694098529502	0.0
36	1.7788640707703282E-4	0.0	0.0	23.64839684322782	0.0
37	1.7788640707703282E-4	0.0	0.0	24.01697747869143	0.0
38	1.7788640707703282E-4	0.0	0.0	24.370437769553497	0.0
39	1.7788640707703282E-4	0.0	0.0	24.775307232060822	0.0
40	1.7788640707703282E-4	0.0	0.0	25.19574175518739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	6.3113956E-4	46.0	10
CAACGTA	20	6.3113956E-4	46.0	10
CGACCGA	30	1.8613664E-6	46.0	20
CGTTCCG	20	6.3113956E-4	46.0	35
AAAGTCG	20	6.3113956E-4	46.0	13
TACGATG	20	6.3113956E-4	46.0	28
ACTCGTA	20	6.3113956E-4	46.0	15
TCGTATC	20	6.3113956E-4	46.0	24
TGCTCGA	20	6.3113956E-4	46.0	30
ACAGACG	20	6.3113956E-4	46.0	15
CGTAATA	20	6.3113956E-4	46.0	13
ATACGGT	20	6.3113956E-4	46.0	30
TGTAACG	20	6.3113956E-4	46.0	21
AGTACGG	105	0.0	43.809525	1
ACGGGAT	400	0.0	43.699997	4
CTCGAGG	170	0.0	43.294117	1
TTACGGG	330	0.0	43.21212	2
CGGGATA	160	0.0	43.125	5
ACTACGG	75	0.0	42.933334	1
CGAATAT	145	0.0	42.827583	14
>>END_MODULE