FastQCFastQC Report
Thu 26 May 2016
SRR1544903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544903_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences692815
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT28190.4068907284051298No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT24500.35362975686149983Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18350.2648614709554499No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT17820.2572115211131399No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.2159306596999199No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTTG13960.2014967920729199Illumina Single End Adapter 1 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11070.15978291463088992No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8550.12340956821084995No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG7540.10883136190757996No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC7500.10825400720249993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG750.046.0000048
CTGTCGG405.6061253E-946.01
GTACCGG253.4157398E-546.01
ATCTCCG206.309709E-446.029
TGCCCCG301.8603241E-646.017
CATGCGG1300.046.01
CTATCGG206.309709E-446.01
ATCACGG301.8603241E-646.01
TACGCTT206.309709E-446.017
CTAACGG501.6370905E-1146.01
ACGATAA206.309709E-446.018
ACCTAGG405.6061253E-946.01
GTTCGGA206.309709E-446.041
CGGGATA600.046.05
GAACGAT301.8603241E-646.08
CTCGCGG301.8603241E-646.01
GCGTAAG253.4157398E-546.04
GCATCGA206.309709E-446.022
CGCTTAA206.309709E-446.019
AATGCGG700.045.9999961
Produced by FastQC (version 0.11.5)