##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544903_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	692815
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92348606770927	34.0	33.0	34.0	31.0	34.0
2	33.28016858757388	34.0	34.0	34.0	31.0	34.0
3	33.40412086920751	34.0	34.0	34.0	31.0	34.0
4	36.62372927837879	37.0	37.0	37.0	35.0	37.0
5	36.629478287854624	37.0	37.0	37.0	35.0	37.0
6	36.43017544366101	37.0	37.0	37.0	35.0	37.0
7	36.22348245924237	37.0	37.0	37.0	35.0	37.0
8	36.35908142866422	37.0	37.0	37.0	35.0	37.0
9	38.25574504016224	39.0	39.0	39.0	37.0	39.0
10	38.129652215959524	39.0	39.0	39.0	35.0	39.0
11	37.542560423778355	39.0	35.0	39.0	35.0	39.0
12	37.532463933373265	39.0	35.0	39.0	35.0	39.0
13	37.530726095710975	39.0	35.0	39.0	35.0	39.0
14	38.76435556389512	41.0	35.0	41.0	35.0	41.0
15	38.78978659526714	41.0	35.0	41.0	35.0	41.0
16	38.80762541226734	41.0	35.0	41.0	35.0	41.0
17	38.8773005780764	41.0	36.0	41.0	35.0	41.0
18	38.87620649091027	41.0	36.0	41.0	35.0	41.0
19	38.84363358183642	41.0	36.0	41.0	35.0	41.0
20	38.76386625578257	41.0	35.0	41.0	35.0	41.0
21	38.712426838333464	41.0	35.0	41.0	35.0	41.0
22	38.69155835251835	41.0	35.0	41.0	35.0	41.0
23	38.641982347379894	41.0	35.0	41.0	35.0	41.0
24	38.60992617076709	41.0	35.0	41.0	35.0	41.0
25	38.55729740262552	40.0	35.0	41.0	35.0	41.0
26	38.47849858908944	40.0	35.0	41.0	35.0	41.0
27	38.557672683183824	41.0	36.0	41.0	35.0	41.0
28	38.595775206945575	41.0	36.0	41.0	35.0	41.0
29	38.595649632297224	40.0	36.0	41.0	35.0	41.0
30	38.55331221177371	41.0	35.0	41.0	35.0	41.0
31	38.477085513448756	40.0	35.0	41.0	35.0	41.0
32	38.41572714216638	40.0	35.0	41.0	35.0	41.0
33	38.348745336056524	40.0	35.0	41.0	35.0	41.0
34	38.25151736033429	40.0	35.0	41.0	35.0	41.0
35	38.160159638575955	40.0	35.0	41.0	35.0	41.0
36	38.142696102134046	40.0	35.0	41.0	35.0	41.0
37	38.06088205365069	40.0	35.0	41.0	35.0	41.0
38	38.00934159912819	40.0	35.0	41.0	35.0	41.0
39	37.92093704668635	40.0	35.0	41.0	35.0	41.0
40	37.87080100748396	40.0	35.0	41.0	35.0	41.0
41	37.81911477089844	40.0	35.0	41.0	35.0	41.0
42	37.755843912155484	40.0	35.0	41.0	35.0	41.0
43	37.68240150689578	40.0	35.0	41.0	35.0	41.0
44	37.49550024176728	40.0	35.0	41.0	35.0	41.0
45	37.513907753152	39.0	35.0	41.0	35.0	41.0
46	37.46338199952368	39.0	35.0	41.0	35.0	41.0
47	37.37753079826505	39.0	35.0	41.0	35.0	41.0
48	37.32620829514372	39.0	35.0	41.0	35.0	41.0
49	37.25984137179478	39.0	35.0	41.0	35.0	41.0
50	37.20154442383609	39.0	35.0	41.0	35.0	41.0
51	37.1076463413754	38.0	35.0	41.0	34.0	41.0
52	36.63372328832372	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	5.0
15	9.0
16	11.0
17	24.0
18	57.0
19	102.0
20	191.0
21	287.0
22	420.0
23	679.0
24	1074.0
25	1781.0
26	2699.0
27	3170.0
28	2970.0
29	3167.0
30	3711.0
31	4520.0
32	6074.0
33	8792.0
34	31802.0
35	133222.0
36	25171.0
37	40714.0
38	74069.0
39	345631.0
40	2458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.385680159927254	21.67259658061676	22.800170319638	22.141552939817988
2	27.816083658696765	24.136890800574466	25.372718546798207	22.67430699393056
3	27.860539970987926	24.6852334317242	24.707028571840965	22.747198025446906
4	26.10884579577521	26.783196091308646	22.50730714548617	24.600650967429978
5	24.75841314059309	36.012066713336175	20.091510720755178	19.13800942531556
6	86.69529383746021	3.1771829420552384	7.0457481434437765	3.0817750770407684
7	85.70267676075143	3.0572375020748686	5.8242099261707665	5.4158758110029375
8	74.56189603285148	5.998282369752387	11.622438890612933	7.817382706783197
9	49.96138940409777	20.52611447500415	14.675490571075972	14.837005549822102
10	39.1613922908713	20.94715039368374	25.568874807849134	14.322582507595824
11	38.3155676479291	17.34936454897772	25.005520954367327	19.32954684872585
12	21.46763566031336	30.4245722162482	28.549035456795824	19.55875666664261
13	16.220636100546322	32.65821323152645	28.929367868767276	22.19178279915995
14	14.561751694175213	23.374638251192597	41.55957939709735	20.504030657534837
15	16.97422832935199	18.6186788680961	42.79757222346514	21.60952057908677
16	18.07639846134971	22.133325635270598	27.963453447168433	31.826822456211257
17	19.96550305637147	23.8581728166971	32.62328327186911	23.553040855062317
18	24.378225067297908	26.837178756233627	26.516746894914228	22.267849281554238
19	26.959433615034317	23.76897151476224	24.974776816321818	24.296818053881626
20	21.45753195297446	30.785274568246933	26.1098561665091	21.64733731226951
21	21.681689917221767	24.14410773438797	24.828417398584037	29.345784949806227
22	19.66960877001797	29.529528084697937	23.90161875825437	26.899244387029725
23	19.48976277938555	22.836832343410578	35.00025259268347	22.6731522845204
24	20.01934138262018	23.325563101260798	32.01114294580805	24.64395257031098
25	18.15809415211853	28.957080894611114	28.417398584037585	24.46742636923277
26	19.54591052445458	28.070985760989586	27.038675548306546	25.344428166249287
27	19.64218442152667	26.764865079422357	33.62355029842021	19.96940020063076
28	18.865642343194068	24.348346961310018	30.268686445876604	26.517324249619307
29	20.18879498856116	28.471958603667645	28.033313366483114	23.30593304128808
30	26.238606265741936	23.618281936736356	23.63646860994638	26.506643187575328
31	27.905573637984165	25.098908077914018	24.745133982376245	22.250384301725568
32	23.717153929981308	26.075214884204296	25.021398208757027	25.186232977057365
33	18.77701839596429	24.515924164459488	29.063025482993293	27.644031956582925
34	22.35055534305695	21.3755475848531	31.074384936815747	25.199512135274205
35	25.566565389028817	22.11946912234868	29.751232291448655	22.56273319717385
36	24.683501367608958	25.278321052517626	28.991000483534567	21.04717709633885
37	22.38288720654143	25.718554015141127	28.216334807993476	23.682223970323967
38	24.343872462345647	25.13167295742731	25.613619797492838	24.910834782734206
39	27.384799694002005	21.332678998000908	26.869510619718106	24.413010688278977
40	20.09324278487042	20.40068416532552	29.54713740320288	29.958935646601187
41	22.43831325822911	24.400164546090945	24.33781023794231	28.823711957737636
42	22.16623485346016	21.93716937421967	27.923038617812836	27.973557154507333
43	22.67445133260683	26.296774752278747	28.667681848689764	22.36109206642466
44	18.94560596984765	28.103028947121526	28.183858605832725	24.767506477198097
45	22.10705599618946	26.298939832422796	26.72531628212438	24.86868788926337
46	23.9823040782893	21.695113414114882	28.972380794295738	25.350201713300084
47	22.70533980932861	19.66556728708241	31.479254923753093	26.149837979835887
48	21.6945360594098	19.00420747241327	29.54713740320288	29.754119064974056
49	20.7779854650953	23.45604526460888	30.787728325743522	24.978240944552297
50	20.82172008400511	24.034410340422767	27.92361597251792	27.220253603054207
51	20.89764222772313	20.75460259953956	31.045228524209207	27.302526648528108
52	23.570505834890987	19.00074334418279	32.2316924431486	25.197058377777616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	268.5
2	444.0
3	1649.5
4	2855.0
5	1989.5
6	1124.0
7	1135.0
8	1146.0
9	1117.0
10	1088.0
11	1072.0
12	1056.0
13	996.0
14	940.0
15	944.0
16	861.5
17	779.0
18	841.5
19	904.0
20	899.0
21	894.0
22	1151.0
23	1408.0
24	1590.0
25	1772.0
26	2272.5
27	2773.0
28	4290.5
29	5808.0
30	5592.5
31	5377.0
32	6557.5
33	7738.0
34	9316.0
35	10894.0
36	11455.0
37	12016.0
38	13813.5
39	18213.0
40	20815.0
41	26496.0
42	32177.0
43	41584.0
44	50991.0
45	58658.5
46	66326.0
47	70472.0
48	74618.0
49	75562.5
50	76507.0
51	68688.0
52	60869.0
53	52435.0
54	44001.0
55	39367.5
56	34734.0
57	31477.0
58	28220.0
59	28285.0
60	28350.0
61	27614.0
62	26878.0
63	25432.0
64	20020.5
65	16055.0
66	12987.5
67	9920.0
68	8435.0
69	6950.0
70	5662.5
71	4375.0
72	4414.0
73	4453.0
74	3644.0
75	2835.0
76	2339.5
77	1844.0
78	1550.0
79	1256.0
80	956.0
81	656.0
82	423.0
83	190.0
84	136.0
85	82.0
86	52.0
87	22.0
88	14.0
89	7.5
90	9.0
91	19.0
92	29.0
93	15.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	692815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.47836565974394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19067191962458	18.39300336390804
2	10.536921652443333	5.369270855780491
3	3.6133524347823345	2.7618694377273116
4	1.7095354410649022	1.7422467630297287
5	1.0578452901301545	1.3476084556686996
6	0.7097236554932482	1.0849559287216268
7	0.6176299783941193	1.1015341702361583
8	0.45080487803705177	0.918861721905942
9	0.4143849936514148	0.9502067152945276
>10	8.140707505188583	51.65344565568055
>50	0.4451439351996077	7.43683048760421
>100	0.10303203615233847	4.33618109324629
>500	0.006261615456771952	1.0352418437217212
>1k	0.003984664381582151	1.8687435074746956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2819	0.4068907284051298	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	2450	0.35362975686149983	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1835	0.2648614709554499	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1782	0.2572115211131399	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1496	0.2159306596999199	No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTTG	1396	0.2014967920729199	Illumina Single End Adapter 1 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1107	0.15978291463088992	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	855	0.12340956821084995	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	754	0.10883136190757996	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	750	0.10825400720249993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3583929331784098	0.0
2	0.0	0.0	0.0	1.2017638186240194	0.0
3	0.0	0.0	0.0	1.874815066071029	0.0
4	0.0	0.0	0.0	2.3121612551691286	0.0
5	0.0	0.0	0.0	4.484169655680088	0.0
6	0.0	0.0	0.0	4.937826115196698	0.0
7	1.4433867626999992E-4	0.0	0.0	7.286938071490947	0.0
8	1.4433867626999992E-4	0.0	0.0	9.689455338005095	0.0
9	1.4433867626999992E-4	0.0	0.0	12.164141942654243	0.0
10	1.4433867626999992E-4	0.0	0.0	13.137417636742853	0.0
11	1.4433867626999992E-4	0.0	0.0	14.093372689679063	0.0
12	1.4433867626999992E-4	0.0	0.0	14.898638164589393	0.0
13	1.4433867626999992E-4	0.0	0.0	15.212430446800372	0.0
14	1.4433867626999992E-4	0.0	0.0	15.440485555306973	0.0
15	1.4433867626999992E-4	0.0	0.0	16.192778736026213	0.0
16	1.4433867626999992E-4	0.0	0.0	16.983610343309543	0.0
17	1.4433867626999992E-4	0.0	0.0	18.03179781038228	0.0
18	1.4433867626999992E-4	0.0	0.0	18.499166444144542	0.0
19	1.4433867626999992E-4	0.0	0.0	19.10264644962941	0.0
20	1.4433867626999992E-4	0.0	0.0	19.79244098352374	0.0
21	1.4433867626999992E-4	0.0	0.0	20.36777494713596	0.0
22	1.4433867626999992E-4	0.0	0.0	21.01888671578993	0.0
23	1.4433867626999992E-4	0.0	0.0	21.48567799484711	0.0
24	1.4433867626999992E-4	0.0	0.0	21.895888512806447	0.0
25	1.4433867626999992E-4	0.0	0.0	22.29253119519641	0.0
26	1.4433867626999992E-4	0.0	0.0	22.66680138276452	0.0
27	1.4433867626999992E-4	0.0	0.0	23.051319616347797	0.0
28	1.4433867626999992E-4	0.0	0.0	23.411588952317718	0.0
29	1.4433867626999992E-4	0.0	0.0	23.890071664152767	0.0
30	1.4433867626999992E-4	0.0	0.0	24.338243253971118	0.0
31	1.4433867626999992E-4	0.0	0.0	24.74643303046268	0.0
32	1.4433867626999992E-4	0.0	0.0	25.154767145630508	0.0
33	1.4433867626999992E-4	0.0	0.0	25.531635429371477	0.0
34	1.4433867626999992E-4	0.0	0.0	26.021665235308127	0.0
35	1.4433867626999992E-4	0.0	0.0	26.407626855654108	0.0
36	1.4433867626999992E-4	0.0	0.0	26.789258315711987	0.0
37	1.4433867626999992E-4	0.0	0.0	27.176807661496937	0.0
38	1.4433867626999992E-4	0.0	0.0	27.541262819078685	0.0
39	1.4433867626999992E-4	0.0	0.0	27.953061062476998	0.0
40	1.4433867626999992E-4	0.0	0.0	28.381169576293814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAG	75	0.0	46.000004	8
CTGTCGG	40	5.6061253E-9	46.0	1
GTACCGG	25	3.4157398E-5	46.0	1
ATCTCCG	20	6.309709E-4	46.0	29
TGCCCCG	30	1.8603241E-6	46.0	17
CATGCGG	130	0.0	46.0	1
CTATCGG	20	6.309709E-4	46.0	1
ATCACGG	30	1.8603241E-6	46.0	1
TACGCTT	20	6.309709E-4	46.0	17
CTAACGG	50	1.6370905E-11	46.0	1
ACGATAA	20	6.309709E-4	46.0	18
ACCTAGG	40	5.6061253E-9	46.0	1
GTTCGGA	20	6.309709E-4	46.0	41
CGGGATA	60	0.0	46.0	5
GAACGAT	30	1.8603241E-6	46.0	8
CTCGCGG	30	1.8603241E-6	46.0	1
GCGTAAG	25	3.4157398E-5	46.0	4
GCATCGA	20	6.309709E-4	46.0	22
CGCTTAA	20	6.309709E-4	46.0	19
AATGCGG	70	0.0	45.999996	1
>>END_MODULE