FastQCFastQC Report
Thu 26 May 2016
SRR1544902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544902_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences643732
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT21990.34160178459358864TruSeq Adapter, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT18540.28800805304070637TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT17790.2763572418335581TruSeq Adapter, Index 8 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.17336407076236696No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT7110.11044969024376604TruSeq Adapter, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGA1150.046.0000045
TCAAAGG1150.046.0000041
CGGGTAG405.6043064E-946.05
CCCCTTA253.415469E-546.036
TTGGCGA206.309375E-446.042
AGATCGA253.415469E-546.014
TCACCGG453.092282E-1046.01
GATGTCG206.309375E-446.040
GTACACC253.415469E-546.08
GATCGAA301.8601168E-646.08
TTTAACG301.8601168E-646.020
CCGTGTT253.415469E-546.034
TAAACGG206.309375E-446.01
TACGGGT253.415469E-546.03
TCGATAT253.415469E-546.014
ACGGGTA453.092282E-1046.04
TTATAGT206.309375E-446.036
ACTAGTC206.309375E-446.029
AATGCGG501.6370905E-1146.01
CCGTACC206.309375E-446.034
Produced by FastQC (version 0.11.5)