##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544902_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	643732
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.918596558816404	34.0	33.0	34.0	31.0	34.0
2	33.21959138274934	34.0	33.0	34.0	31.0	34.0
3	33.27091553627907	34.0	34.0	34.0	31.0	34.0
4	36.55664624408916	37.0	37.0	37.0	35.0	37.0
5	36.550597142910405	37.0	37.0	37.0	35.0	37.0
6	36.658374913783994	37.0	37.0	37.0	35.0	37.0
7	36.50627124331243	37.0	37.0	37.0	35.0	37.0
8	36.534710096748334	37.0	37.0	37.0	35.0	37.0
9	38.32817694319996	39.0	39.0	39.0	37.0	39.0
10	38.098475763205805	39.0	39.0	39.0	35.0	39.0
11	37.280585709581004	39.0	35.0	39.0	35.0	39.0
12	37.17454313285653	39.0	35.0	39.0	35.0	39.0
13	37.156468841070506	39.0	35.0	39.0	35.0	39.0
14	38.417344484972006	40.0	35.0	41.0	35.0	41.0
15	38.48465355147795	40.0	35.0	41.0	35.0	41.0
16	38.513168523547066	40.0	35.0	41.0	35.0	41.0
17	38.588269652588345	40.0	36.0	41.0	35.0	41.0
18	38.568879595856664	40.0	36.0	41.0	35.0	41.0
19	38.456284602909285	40.0	35.0	41.0	35.0	41.0
20	38.353816495063164	40.0	35.0	41.0	35.0	41.0
21	38.3048955155251	40.0	35.0	41.0	35.0	41.0
22	38.28493223888202	40.0	35.0	41.0	35.0	41.0
23	38.25213132173016	40.0	35.0	41.0	35.0	41.0
24	38.20268372552553	40.0	35.0	41.0	35.0	41.0
25	38.148875619046436	40.0	35.0	41.0	35.0	41.0
26	38.12264265253242	40.0	35.0	41.0	35.0	41.0
27	38.07644640937533	40.0	35.0	41.0	35.0	41.0
28	38.10686279383346	40.0	35.0	41.0	35.0	41.0
29	38.099145296489844	40.0	35.0	41.0	35.0	41.0
30	37.99460334424885	40.0	35.0	41.0	35.0	41.0
31	37.93062019598218	40.0	35.0	41.0	35.0	41.0
32	37.866638601157	40.0	35.0	41.0	35.0	41.0
33	37.77853205992556	40.0	35.0	41.0	35.0	41.0
34	37.71094492739214	40.0	35.0	41.0	35.0	41.0
35	37.637825989697575	40.0	35.0	41.0	35.0	41.0
36	37.55873872978196	40.0	35.0	41.0	35.0	41.0
37	37.49414973933252	40.0	35.0	41.0	35.0	41.0
38	37.50572132502346	39.0	35.0	41.0	35.0	41.0
39	37.409765243921385	39.0	35.0	41.0	34.0	41.0
40	37.364448248650056	39.0	35.0	41.0	34.0	41.0
41	37.34544344540896	39.0	35.0	41.0	34.0	41.0
42	37.2305322711936	39.0	35.0	41.0	34.0	41.0
43	37.14742781157376	39.0	35.0	41.0	34.0	41.0
44	37.05047597447385	38.0	35.0	41.0	34.0	41.0
45	37.003071153834206	38.0	35.0	41.0	34.0	41.0
46	37.03283353942324	38.0	35.0	41.0	34.0	41.0
47	37.00051884945909	38.0	35.0	41.0	34.0	41.0
48	37.02244101582646	38.0	35.0	41.0	34.0	41.0
49	36.93021474775217	38.0	35.0	41.0	34.0	41.0
50	36.85315938931108	38.0	35.0	41.0	34.0	41.0
51	36.788309420690595	37.0	35.0	41.0	34.0	41.0
52	36.5958675349369	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	9.0
15	11.0
16	10.0
17	16.0
18	45.0
19	113.0
20	216.0
21	312.0
22	481.0
23	761.0
24	1346.0
25	2238.0
26	2949.0
27	3576.0
28	3537.0
29	3918.0
30	4610.0
31	5522.0
32	7342.0
33	10676.0
34	33398.0
35	151393.0
36	22733.0
37	33541.0
38	60891.0
39	291881.0
40	2202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.659398631728735	22.094598373236067	24.472761956839182	20.77324103819602
2	27.264762354520204	24.04556554591041	26.406485928926944	22.28318617064244
3	26.391883578880655	25.30944554566186	25.331038382432443	22.967632493025047
4	26.175023146278264	27.89670235439593	22.935786942392173	22.99248755693363
5	23.387061696482387	36.358142829624754	20.50154412084532	19.753251353047542
6	87.45021220010813	2.988666090857686	6.998098587610993	2.563023121423201
7	87.30760627093262	2.3620077920625353	5.414520328335394	4.9158656086694466
8	78.0223757712837	4.502184138740967	10.836497175843364	6.638942914131968
9	53.49928231002964	17.893160507788956	13.92023388615138	14.687323296030025
10	44.60520837864204	19.42935880148882	23.78551943976686	12.179913380102278
11	41.751070321189566	17.069215139219427	23.06254776832595	18.11716677126506
12	20.41921172164814	35.8562258828208	26.733640707623668	16.990921687907388
13	13.268720523447646	39.36203264712644	26.66622134677164	20.703025482654272
14	11.796524019312384	22.296701111642733	47.61857418925889	18.288200679785998
15	12.944206595291208	17.40242833974387	49.189880260729616	20.463484804235303
16	13.905165503656802	19.960946480833638	28.96795560885586	37.1659324066537
17	17.60810399358739	22.51961996607284	34.95072483580123	24.921551204538535
18	24.51548159793206	26.852168293637725	27.04526107137753	21.587089037052685
19	27.842953278693617	21.970944430290867	24.899492335319668	25.286609955695848
20	18.850235812418834	31.5859705591768	26.405553864030374	23.158239764373995
21	19.941683806304486	24.64301914461298	24.430974380642876	30.984322668439663
22	17.260599131315516	31.45206390236931	22.028887798027753	29.258449168287427
23	17.713427326900014	22.07921930244263	38.34608190986311	21.861271460794242
24	17.843916412420075	21.78468679512592	35.550819285044085	24.820577507409915
25	15.9480964127929	31.603524448062238	28.853777659025802	23.594601480119056
26	18.273287641440845	30.255603263469894	26.284696115774885	25.186412979314373
27	18.598733634493858	27.207440363381036	36.33965687584274	17.854169126282365
28	17.12715850695631	24.106460452486438	32.34063243710115	26.425748603456096
29	19.895857282223037	27.707027147943553	27.17637153349531	25.220744036338104
30	26.94086980296148	26.560276636861303	21.12059055631847	25.37826300385875
31	30.38314081015081	24.084867615715858	22.45655024140481	23.075441332728527
32	25.71287430172805	27.412805328925703	22.21778628373298	24.65653408561327
33	18.99035623520347	28.300131110462118	24.626241976474684	28.08327067785973
34	21.63136211963985	23.67662319101738	27.22872251185276	27.46329217749001
35	31.285690318331234	22.145085221800375	25.322649798363294	21.2465746615051
36	22.74750983328466	28.206147900057786	24.846986012812785	24.199356253844766
37	20.06642515829569	29.077162545904194	25.816644193546384	25.039768102253735
38	18.53675131887183	26.474682010526116	26.51413942448099	28.47442724612106
39	24.860500953813077	21.272206446160823	27.133962580701287	26.733330019324814
40	15.086247071762784	20.31062616119752	30.37863582981738	34.22449093722232
41	21.764181367401342	23.809442438778873	24.099159277463293	30.327216916356498
42	23.017963997439928	22.909689125288164	26.962928672180347	27.10941820509156
43	23.793597335537147	27.201847974001602	26.334250899442623	22.670303791018622
44	17.504644790067918	31.74846053947916	24.944542138653976	25.80235253179895
45	22.317206539367316	28.50378729036307	25.157674311670075	24.02133185859954
46	22.98099208987591	22.531736809728272	27.0781940310564	27.409077069339418
47	23.719498176259684	19.274014652060174	30.370713278196515	26.635773893483623
48	20.471096667557305	19.008376156537192	28.044900672950856	32.47562650295465
49	20.431949941901287	25.502538323401662	28.954129979556708	25.11138175514034
50	19.053115271572644	27.235557654427616	25.934395058813298	27.776932015186446
51	19.90797412587847	21.74134577743533	30.3315665525405	28.019113544145704
52	23.564464715129898	18.722232233289628	32.00353563284099	25.709767418739478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	190.5
2	215.0
3	1084.5
4	1954.0
5	1420.0
6	886.0
7	975.5
8	1065.0
9	1154.0
10	1243.0
11	1184.0
12	1125.0
13	1095.0
14	1004.0
15	943.0
16	860.5
17	778.0
18	762.5
19	747.0
20	736.0
21	725.0
22	831.0
23	937.0
24	1220.0
25	1503.0
26	2060.5
27	2618.0
28	3157.0
29	3696.0
30	4295.5
31	4895.0
32	5422.0
33	5949.0
34	7233.5
35	8518.0
36	9513.0
37	10508.0
38	11641.5
39	14803.0
40	16831.0
41	23772.5
42	30714.0
43	42559.5
44	54405.0
45	57739.0
46	61073.0
47	61413.0
48	61753.0
49	65980.0
50	70207.0
51	71889.0
52	73571.0
53	66054.5
54	58538.0
55	48247.0
56	37956.0
57	31834.5
58	25713.0
59	23376.0
60	21039.0
61	20277.0
62	19515.0
63	18064.0
64	14234.5
65	11856.0
66	9288.5
67	6721.0
68	5742.5
69	4764.0
70	4202.0
71	3640.0
72	3050.0
73	2460.0
74	1969.0
75	1478.0
76	1582.5
77	1687.0
78	1047.0
79	407.0
80	375.5
81	344.0
82	221.0
83	98.0
84	65.0
85	32.0
86	17.0
87	2.0
88	2.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	643732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.26363060759062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.90627931621542	16.26273859163488
2	12.14855058581794	5.652387864921945
3	4.572634063235327	3.1912820925237853
4	2.0879262867866246	1.9429098348672944
5	1.228589407602515	1.4290725073431747
6	0.8089634303481025	1.1291655851200575
7	0.5361478936076094	0.8730922583547654
8	0.4006137965796127	0.7455785103946049
9	0.35145925782072224	0.7358596512803315
>10	6.565808363545611	42.58621615616858
>50	1.230440803809891	17.954998886789962
>100	0.15388873565865957	5.608320242125727
>500	0.006021733134469288	0.8069032814848263
>1k	0.002676325837541906	1.0814745369900796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2199	0.34160178459358864	TruSeq Adapter, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1854	0.28800805304070637	TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	1779	0.2763572418335581	TruSeq Adapter, Index 8 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1116	0.17336407076236696	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	711	0.11044969024376604	TruSeq Adapter, Index 13 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.281172910465846	0.0
2	0.0	0.0	0.0	1.1183225317368097	0.0
3	0.0	0.0	0.0	1.7760496604176894	0.0
4	0.0	0.0	0.0	2.2183144538410393	0.0
5	0.0	0.0	0.0	4.6244399843413095	0.0
6	0.0	0.0	0.0	5.109424418857537	0.0
7	0.0	0.0	0.0	7.797654924720225	0.0
8	0.0	0.0	0.0	10.506856890755781	0.0
9	0.0	0.0	0.0	13.344217780069966	0.0
10	0.0	0.0	0.0	14.350692524218154	0.0
11	0.0	0.0	0.0	15.360274151354911	0.0
12	0.0	0.0	0.0	16.21264749926988	0.0
13	0.0	0.0	0.0	16.511218954471737	0.0
14	0.0	0.0	0.0	16.72854541952241	0.0
15	0.0	0.0	0.0	17.555286982781656	0.0
16	0.0	0.0	0.0	18.42536956373149	0.0
17	0.0	0.0	0.0	19.75822236582926	0.0
18	0.0	0.0	0.0	20.25408089080549	0.0
19	0.0	0.0	0.0	20.9163129998198	0.0
20	0.0	0.0	0.0	21.695363909204453	0.0
21	0.0	0.0	0.0	22.305711072309595	0.0
22	0.0	0.0	0.0	23.082431819452815	0.0
23	1.5534414942864423E-4	0.0	0.0	23.615106907843636	0.0
24	1.5534414942864423E-4	0.0	0.0	24.04665295495641	0.0
25	1.5534414942864423E-4	0.0	0.0	24.49932580639148	0.0
26	1.5534414942864423E-4	0.0	0.0	24.877588810250227	0.0
27	1.5534414942864423E-4	0.0	0.0	25.276667930132415	0.0
28	1.5534414942864423E-4	0.0	0.0	25.67605773831346	0.0
29	1.5534414942864423E-4	0.0	0.0	26.228927566130004	0.0
30	1.5534414942864423E-4	0.0	0.0	26.763777472612826	0.0
31	1.5534414942864423E-4	0.0	0.0	27.17341999465616	0.0
32	1.5534414942864423E-4	0.0	0.0	27.566906725158915	0.0
33	1.5534414942864423E-4	0.0	0.0	27.944859040718807	0.0
34	1.5534414942864423E-4	0.0	0.0	28.5061174526045	0.0
35	1.5534414942864423E-4	0.0	0.0	28.946673460384137	0.0
36	1.5534414942864423E-4	0.0	0.0	29.37371452716348	0.0
37	1.5534414942864423E-4	0.0	0.0	29.75384166081537	0.0
38	1.5534414942864423E-4	0.0	0.0	30.123560736455545	0.0
39	1.5534414942864423E-4	0.0	0.0	30.559767108051176	0.0
40	1.5534414942864423E-4	0.0	0.0	30.992866596658235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGCGA	115	0.0	46.000004	5
TCAAAGG	115	0.0	46.000004	1
CGGGTAG	40	5.6043064E-9	46.0	5
CCCCTTA	25	3.415469E-5	46.0	36
TTGGCGA	20	6.309375E-4	46.0	42
AGATCGA	25	3.415469E-5	46.0	14
TCACCGG	45	3.092282E-10	46.0	1
GATGTCG	20	6.309375E-4	46.0	40
GTACACC	25	3.415469E-5	46.0	8
GATCGAA	30	1.8601168E-6	46.0	8
TTTAACG	30	1.8601168E-6	46.0	20
CCGTGTT	25	3.415469E-5	46.0	34
TAAACGG	20	6.309375E-4	46.0	1
TACGGGT	25	3.415469E-5	46.0	3
TCGATAT	25	3.415469E-5	46.0	14
ACGGGTA	45	3.092282E-10	46.0	4
TTATAGT	20	6.309375E-4	46.0	36
ACTAGTC	20	6.309375E-4	46.0	29
AATGCGG	50	1.6370905E-11	46.0	1
CCGTACC	20	6.309375E-4	46.0	34
>>END_MODULE