FastQCFastQC Report
Thu 26 May 2016
SRR1544901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544901_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences383195
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT19720.5146204934824307TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT14480.3778754942000809TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT11940.3115907044716137TruSeq Adapter, Index 13 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTTG8310.216860867182505TruSeq Adapter, Index 13 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.17823823379741385No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6240.16284137319119507No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT5110.13335247067419984TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC4880.1273503046751654TruSeq Adapter, Index 8 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC4510.1176946463288926TruSeq Adapter, Index 8 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4160.10856091546079673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC206.3061604E-446.00000424
GTCATCG206.3061604E-446.00000443
GACAGCG206.3061604E-446.0000049
CTGTCGG206.3061604E-446.0000041
ACGTTGG206.3061604E-446.0000041
TCCGATG206.3061604E-446.00000433
CGCATGT206.3061604E-446.00000438
ATTACAT206.3061604E-446.00000442
TTCACGG206.3061604E-446.0000041
GAATCTA206.3061604E-446.0000048
CAATTGC206.3061604E-446.00000424
TGCGGAT206.3061604E-446.00000417
ACCTCCG206.3061604E-446.00000417
ATGTAGG800.046.0000041
CTAACGG206.3061604E-446.0000041
CCCGTAC206.3061604E-446.00000418
CCGTACC206.3061604E-446.00000419
TACGAGA206.3061604E-446.00000434
CGGGCAC405.5952114E-946.0000045
ACAGCGC206.3061604E-446.00000410
Produced by FastQC (version 0.11.5)