##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544901_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	383195
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9194665901173	34.0	33.0	34.0	31.0	34.0
2	33.26803846605514	34.0	33.0	34.0	31.0	34.0
3	33.38561306906406	34.0	34.0	34.0	31.0	34.0
4	36.61662339017993	37.0	37.0	37.0	35.0	37.0
5	36.61057164107047	37.0	37.0	37.0	35.0	37.0
6	36.42087970876447	37.0	37.0	37.0	35.0	37.0
7	36.26165268335965	37.0	37.0	37.0	35.0	37.0
8	36.39612990774932	37.0	37.0	37.0	35.0	37.0
9	38.27285585667872	39.0	39.0	39.0	37.0	39.0
10	38.118756768746984	39.0	39.0	39.0	35.0	39.0
11	37.37597045890473	39.0	35.0	39.0	35.0	39.0
12	37.33074283328332	39.0	35.0	39.0	35.0	39.0
13	37.31908297342084	39.0	35.0	39.0	35.0	39.0
14	38.5294301856757	41.0	35.0	41.0	35.0	41.0
15	38.56204543378698	41.0	35.0	41.0	35.0	41.0
16	38.6222158431086	41.0	35.0	41.0	35.0	41.0
17	38.717402366941116	41.0	36.0	41.0	35.0	41.0
18	38.71543730998578	41.0	36.0	41.0	35.0	41.0
19	38.63229426271219	41.0	35.0	41.0	35.0	41.0
20	38.530881144065035	40.0	35.0	41.0	35.0	41.0
21	38.483683242213495	40.0	35.0	41.0	35.0	41.0
22	38.46158483278749	40.0	35.0	41.0	35.0	41.0
23	38.415235063087984	40.0	35.0	41.0	35.0	41.0
24	38.384078602278215	40.0	35.0	41.0	35.0	41.0
25	38.332016336330064	40.0	35.0	41.0	35.0	41.0
26	38.28615717846005	40.0	35.0	41.0	35.0	41.0
27	38.35304218478842	40.0	35.0	41.0	35.0	41.0
28	38.367439554273936	40.0	35.0	41.0	35.0	41.0
29	38.36199585067655	40.0	36.0	41.0	35.0	41.0
30	38.27762105455447	40.0	35.0	41.0	35.0	41.0
31	38.21007059069142	40.0	35.0	41.0	35.0	41.0
32	38.166411357141925	40.0	35.0	41.0	35.0	41.0
33	38.09399391954488	40.0	35.0	41.0	35.0	41.0
34	38.00732264251882	40.0	35.0	41.0	35.0	41.0
35	37.938950142877644	40.0	35.0	41.0	35.0	41.0
36	37.86936416184971	40.0	35.0	41.0	35.0	41.0
37	37.80253656754394	40.0	35.0	41.0	35.0	41.0
38	37.80818121322042	40.0	35.0	41.0	35.0	41.0
39	37.74291157243701	40.0	35.0	41.0	35.0	41.0
40	37.71073735304479	40.0	35.0	41.0	35.0	41.0
41	37.69042654523154	40.0	35.0	41.0	35.0	41.0
42	37.616688631114705	40.0	35.0	41.0	35.0	41.0
43	37.53938334268453	39.0	35.0	41.0	35.0	41.0
44	37.39800101775858	39.0	35.0	41.0	35.0	41.0
45	37.39819152128812	39.0	35.0	41.0	35.0	41.0
46	37.35320398230666	39.0	35.0	41.0	35.0	41.0
47	37.29385299912577	39.0	35.0	41.0	35.0	41.0
48	37.26041571523637	39.0	35.0	41.0	35.0	41.0
49	37.18019807147797	39.0	35.0	41.0	35.0	41.0
50	37.107757147144405	38.0	35.0	41.0	35.0	41.0
51	37.03997703519096	38.0	35.0	41.0	34.0	41.0
52	36.59657876538055	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	2.0
16	11.0
17	16.0
18	22.0
19	56.0
20	91.0
21	151.0
22	224.0
23	399.0
24	645.0
25	1126.0
26	1603.0
27	1894.0
28	1831.0
29	1850.0
30	2095.0
31	2489.0
32	3345.0
33	5263.0
34	18017.0
35	87298.0
36	12541.0
37	19563.0
38	36931.0
39	184588.0
40	1140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.745195005154038	21.886245906131343	23.684807995928967	22.683751092785656
2	26.822635994728532	24.458826446065316	26.550189851120187	22.16834770808596
3	26.204151933088898	25.208575268466447	25.463536841555868	23.12373595688879
4	26.17127050196375	27.56768746982607	23.336682367984967	22.92435966022521
5	23.546236250472997	35.86868304649069	20.69207583606258	19.893004866973733
6	86.09741776380172	3.045446835162254	7.622489855034644	3.2346455460013828
7	85.93222771695874	2.243244301204348	6.123775101449653	5.70075288038727
8	76.07510536410965	4.609402523519357	11.981367189029084	7.334124923341902
9	52.459452759039124	17.503359908140766	14.511149675752556	15.526037657067551
10	43.21768290296063	19.17091820091598	25.204138884901944	12.40726001122144
11	40.341079606988615	16.947246180143267	23.685590887146233	19.026083325721892
12	20.029488902516995	34.34308902777959	27.98705619854121	17.640365871162203
13	13.22773000691554	37.917248398335055	27.40536802411305	21.44965357063636
14	11.833400748965932	22.020642231761897	46.97216821722622	19.173788802045955
15	12.915617374965748	17.389840681637285	48.64807734965226	21.046464593744698
16	13.791411683346599	20.433721734365008	29.380341601534465	36.39452498075392
17	17.12574537767977	22.671746760787588	35.07770195331359	25.124805908219056
18	23.96978039901356	27.210428111013975	27.097169848249585	21.722621641722885
19	27.008442176959512	22.032646563759968	25.02563968736544	25.933271571915085
20	18.45196309972729	32.09749605292345	26.117772935450617	23.332767911898642
21	19.66753219640131	24.542595806312715	24.5903521705659	31.199519826720078
22	17.2155169039262	31.987630318767206	22.022990905413693	28.773861871892905
23	17.337126006341418	22.32544787901721	37.81938699617688	22.51803911846449
24	18.07930688030898	22.080141964274063	34.56490820600477	25.27564294941218
25	15.938360364827306	31.32843591382977	28.958624199167527	23.774579522175394
26	18.61637025535302	28.75167995407038	26.160310024922033	26.471639765654565
27	19.171962055872335	26.839076710291103	35.8391941439737	18.149767089862863
28	17.663591643941075	24.161849710982658	31.534336303970562	26.6402223411057
29	20.37683163924373	27.05463275877817	26.465898563394617	26.102637038583488
30	26.480512532783568	26.382912094364485	20.6124818956406	26.52409347721134
31	30.632445621680866	23.51935698534689	21.41546732081577	24.432730072156474
32	25.24693693811245	26.796539620819686	22.348151724317905	25.608371716749957
33	18.939965291822702	27.65197875755164	24.59870301021673	28.809352940408928
34	22.40634663813463	23.173580031054687	27.11543730998578	27.30463602082491
35	31.111053119169092	21.911037461344744	25.200224428815616	21.777684990670547
36	23.025091663513354	27.829695063870872	23.71951617322773	25.42569709938804
37	20.118216573807068	29.054136927673902	26.183796761439996	24.643849737079034
38	18.68187215386422	25.981288899907355	25.903782669398083	29.43305627683033
39	24.703871397069378	20.680854395281774	27.47165281384152	27.143621393807333
40	15.200615874424248	19.888307519670143	29.8044076775532	35.106668928352406
41	21.672777567556988	23.956471248320046	23.647490181239316	30.72326100288365
42	22.66626652226673	22.707498793042706	26.987826041571523	27.63840864311904
43	24.60026879265126	26.56480382050914	26.120382572841507	22.714544813998096
44	17.733007998538604	30.28327613878052	25.82914704001879	26.15456882266209
45	22.09449496992393	27.70573728780386	25.541564999543315	24.6582027427289
46	23.248998551651248	21.07282193139263	27.627709129816413	28.050470387139708
47	23.25708842756299	18.48145200224429	30.776497605657692	27.484961964535024
48	20.329858166207806	18.197001526637873	27.849006380563416	33.6241339265909
49	20.223384960659715	24.29180965304871	29.495426610472474	25.989378775819098
50	19.29409308576573	26.139171962055872	26.553321415989252	28.013413536189148
51	19.742428789519696	20.89432273385613	30.447422330667155	28.91582614595702
52	23.534231918474926	18.159422748209135	32.332624381842145	25.973720951473794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	129.0
2	166.0
3	708.0
4	1250.0
5	882.5
6	515.0
7	596.0
8	677.0
9	692.0
10	707.0
11	715.5
12	724.0
13	689.5
14	598.5
15	542.0
16	499.5
17	457.0
18	465.0
19	473.0
20	443.5
21	414.0
22	488.0
23	562.0
24	718.5
25	875.0
26	1116.5
27	1358.0
28	1657.0
29	1956.0
30	2213.0
31	2470.0
32	2760.5
33	3051.0
34	3747.5
35	4444.0
36	5000.5
37	5557.0
38	6382.5
39	8162.5
40	9117.0
41	12965.0
42	16813.0
43	23644.5
44	30476.0
45	32183.5
46	33891.0
47	34824.5
48	35758.0
49	39972.0
50	44186.0
51	45680.0
52	47174.0
53	42284.0
54	37394.0
55	30584.5
56	23775.0
57	19772.0
58	15769.0
59	14187.5
60	12606.0
61	12136.0
62	11666.0
63	10926.5
64	8696.5
65	7206.0
66	5614.5
67	4023.0
68	3535.0
69	3047.0
70	2568.5
71	2090.0
72	1785.5
73	1481.0
74	1206.0
75	931.0
76	938.5
77	946.0
78	594.0
79	242.0
80	213.0
81	184.0
82	121.0
83	58.0
84	38.0
85	18.0
86	10.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	383195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.759768254327078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12111918250476	18.496651308035492
2	11.585196046061554	6.2003432274710715
3	4.024214462782077	3.2306113928927918
4	2.001750729051527	2.1426554244939653
5	1.0846794478306738	1.4512885327090144
6	0.7860355845962073	1.2620478050069408
7	0.7051341702700288	1.3208458889243224
8	0.548326660162863	1.17384754829019
9	0.5163659026176074	1.2436048699635238
>10	9.246645108742634	52.96586099947227
>50	0.3086210130247951	5.49933672389877
>100	0.06492027782074977	3.0717280796932624
>500	0.003995094019738448	0.7079968400859177
>1k	0.002996320514803836	1.2331813590624479
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1972	0.5146204934824307	TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	1448	0.3778754942000809	TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1194	0.3115907044716137	TruSeq Adapter, Index 13 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTTG	831	0.216860867182505	TruSeq Adapter, Index 13 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.17823823379741385	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	624	0.16284137319119507	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	511	0.13335247067419984	TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	488	0.1273503046751654	TruSeq Adapter, Index 8 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	451	0.1176946463288926	TruSeq Adapter, Index 8 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	416	0.10856091546079673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38466055141638067	0.0
2	0.0	0.0	0.0	1.4572215190699251	0.0
3	0.0	0.0	0.0	2.2495074309424705	0.0
4	0.0	0.0	0.0	2.7534284111222744	0.0
5	0.0	0.0	0.0	5.473975391119404	0.0
6	0.0	0.0	0.0	6.027740445465103	0.0
7	0.0	0.0	0.0	9.1264238834014	0.0
8	0.0	0.0	0.0	12.12724591917953	0.0
9	0.0	0.0	0.0	15.373895797178982	0.0
10	0.0	0.0	0.0	16.500998186302013	0.0
11	0.0	0.0	0.0	17.604613838907085	0.0
12	0.0	0.0	0.0	18.608280379441275	0.0
13	0.0	0.0	0.0	18.94387974790903	0.0
14	0.0	0.0	0.0	19.221284202560053	0.0
15	0.0	0.0	0.0	20.111953444068945	0.0
16	0.0	0.0	0.0	21.053510614700087	0.0
17	0.0	0.0	0.0	22.404519891961012	0.0
18	0.0	0.0	0.0	22.985947102650087	0.0
19	0.0	0.0	0.0	23.66340896932371	0.0
20	0.0	0.0	0.0	24.475006197888803	0.0
21	0.0	0.0	0.0	25.124023017001786	0.0
22	0.0	0.0	0.0	25.92857422461149	0.0
23	0.0	0.0	0.0	26.456242905048345	0.0
24	0.0	0.0	0.0	26.910058847323164	0.0
25	0.0	0.0	0.0	27.371964665509726	0.0
26	0.0	0.0	0.0	27.7545375070134	0.0
27	0.0	0.0	0.0	28.16477250486045	0.0
28	2.6096373908845365E-4	0.0	0.0	28.56013256957946	0.0
29	2.6096373908845365E-4	0.0	0.0	29.11807304375057	0.0
30	2.6096373908845365E-4	0.0	0.0	29.67183809809627	0.0
31	2.6096373908845365E-4	0.0	0.0	30.102689231331308	0.0
32	2.6096373908845365E-4	0.0	0.0	30.492830021268546	0.0
33	2.6096373908845365E-4	0.0	0.0	30.868095878077742	0.0
34	2.6096373908845365E-4	0.0	0.0	31.427080207205208	0.0
35	2.6096373908845365E-4	0.0	0.0	31.84018580618223	0.0
36	2.6096373908845365E-4	0.0	0.0	32.26973212072183	0.0
37	2.6096373908845365E-4	0.0	0.0	32.642649303879224	0.0
38	2.6096373908845365E-4	0.0	0.0	33.02235154425293	0.0
39	2.6096373908845365E-4	0.0	0.0	33.44981014887981	0.0
40	2.6096373908845365E-4	0.0	0.0	33.880139354636675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	6.3061604E-4	46.000004	24
GTCATCG	20	6.3061604E-4	46.000004	43
GACAGCG	20	6.3061604E-4	46.000004	9
CTGTCGG	20	6.3061604E-4	46.000004	1
ACGTTGG	20	6.3061604E-4	46.000004	1
TCCGATG	20	6.3061604E-4	46.000004	33
CGCATGT	20	6.3061604E-4	46.000004	38
ATTACAT	20	6.3061604E-4	46.000004	42
TTCACGG	20	6.3061604E-4	46.000004	1
GAATCTA	20	6.3061604E-4	46.000004	8
CAATTGC	20	6.3061604E-4	46.000004	24
TGCGGAT	20	6.3061604E-4	46.000004	17
ACCTCCG	20	6.3061604E-4	46.000004	17
ATGTAGG	80	0.0	46.000004	1
CTAACGG	20	6.3061604E-4	46.000004	1
CCCGTAC	20	6.3061604E-4	46.000004	18
CCGTACC	20	6.3061604E-4	46.000004	19
TACGAGA	20	6.3061604E-4	46.000004	34
CGGGCAC	40	5.5952114E-9	46.000004	5
ACAGCGC	20	6.3061604E-4	46.000004	10
>>END_MODULE