FastQCFastQC Report
Thu 26 May 2016
SRR1544900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544900_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences732607
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT33370.4554966032265594No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT27330.3730513085460554Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT23740.32404822776741143No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.1775849807604896No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT12020.16407159636749308No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC11410.1557451676000912No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC9520.12994688830437057No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC8930.12189345720147364No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC7940.10838007280847713No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT7820.10674208682144724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC501.6370905E-1146.05
AGTCCGG206.3099485E-446.010
TCTTGCG206.3099485E-446.021
TCGCAGG501.6370905E-1146.01
CGTATGA206.3099485E-446.020
GTCGAGT206.3099485E-446.013
CGAAACA206.3099485E-446.034
CGTGCGG206.3099485E-446.01
GCGCGAC206.3099485E-446.08
TAGAAGT253.4159348E-546.08
ACTCTGC206.3099485E-446.038
ACTTCGA206.3099485E-446.015
CGATTGA206.3099485E-446.09
TGAGTCA206.3099485E-446.012
CGCGAAG206.3099485E-446.013
CGCGAAA206.3099485E-446.017
ATCGTCT253.4159348E-546.039
ATCCGAC206.3099485E-446.019
ATCCGAA206.3099485E-446.027
ATTCCGG405.6061253E-946.01
Produced by FastQC (version 0.11.5)