##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544900_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	732607
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.906413670631046	34.0	33.0	34.0	31.0	34.0
2	33.18872738043726	34.0	33.0	34.0	31.0	34.0
3	33.23091234454489	34.0	33.0	34.0	31.0	34.0
4	36.52437527896949	37.0	37.0	37.0	35.0	37.0
5	36.52140369939135	37.0	37.0	37.0	35.0	37.0
6	36.6507977674251	37.0	37.0	37.0	35.0	37.0
7	36.48672344108096	37.0	37.0	37.0	35.0	37.0
8	36.524453083303875	37.0	37.0	37.0	35.0	37.0
9	38.29417955329392	39.0	39.0	39.0	37.0	39.0
10	38.048777857705424	39.0	39.0	39.0	35.0	39.0
11	37.12259506119925	39.0	35.0	39.0	35.0	39.0
12	36.97444332363737	39.0	35.0	39.0	35.0	39.0
13	36.95794880474798	39.0	35.0	39.0	35.0	39.0
14	38.167615106052764	40.0	35.0	41.0	35.0	41.0
15	38.26944596488977	40.0	35.0	41.0	35.0	41.0
16	38.2888274340813	40.0	35.0	41.0	35.0	41.0
17	38.39267711064732	40.0	36.0	41.0	35.0	41.0
18	38.370711718561246	40.0	36.0	41.0	35.0	41.0
19	38.24914995352215	40.0	35.0	41.0	35.0	41.0
20	38.13719088133201	40.0	35.0	41.0	35.0	41.0
21	38.08798168731666	40.0	35.0	41.0	35.0	41.0
22	38.07412023090142	40.0	35.0	41.0	35.0	41.0
23	38.072014053919766	40.0	35.0	41.0	35.0	41.0
24	38.02091708105437	40.0	35.0	41.0	35.0	41.0
25	37.98985404179867	40.0	35.0	41.0	35.0	41.0
26	37.95238920731033	40.0	35.0	41.0	35.0	41.0
27	37.94516159414256	40.0	35.0	41.0	35.0	41.0
28	37.97684024313172	40.0	35.0	41.0	35.0	41.0
29	37.993812507933995	40.0	35.0	41.0	35.0	41.0
30	37.889977846239525	40.0	35.0	41.0	35.0	41.0
31	37.80057520607911	40.0	35.0	41.0	35.0	41.0
32	37.70298809593684	40.0	35.0	41.0	35.0	41.0
33	37.64897687300285	40.0	35.0	41.0	35.0	41.0
34	37.47667985700382	39.0	35.0	41.0	35.0	41.0
35	37.45798907190349	39.0	35.0	41.0	35.0	41.0
36	37.35720652409819	39.0	35.0	41.0	34.0	41.0
37	37.30982231947006	39.0	35.0	41.0	34.0	41.0
38	37.259021549070646	39.0	35.0	41.0	35.0	41.0
39	37.20543210752832	39.0	35.0	41.0	34.0	41.0
40	37.18456007108859	39.0	35.0	41.0	34.0	41.0
41	37.17930213607023	39.0	35.0	41.0	34.0	41.0
42	37.07654172018558	38.0	35.0	41.0	34.0	41.0
43	36.9915889419566	38.0	35.0	41.0	34.0	41.0
44	36.900732589232696	38.0	35.0	41.0	34.0	41.0
45	36.8722084282569	38.0	35.0	41.0	34.0	41.0
46	36.88824704104656	38.0	35.0	41.0	34.0	41.0
47	36.87531377669064	38.0	35.0	41.0	34.0	41.0
48	36.91352798976805	38.0	35.0	41.0	34.0	41.0
49	36.81720212883579	37.0	35.0	41.0	34.0	41.0
50	36.74543104283743	37.0	35.0	41.0	34.0	41.0
51	36.67151965514935	37.0	35.0	41.0	34.0	41.0
52	36.4974399644011	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	9.0
14	12.0
15	11.0
16	6.0
17	31.0
18	65.0
19	119.0
20	206.0
21	339.0
22	533.0
23	1010.0
24	1580.0
25	2719.0
26	3664.0
27	4201.0
28	4142.0
29	4509.0
30	5333.0
31	6727.0
32	8838.0
33	13217.0
34	39843.0
35	192452.0
36	24216.0
37	34200.0
38	64019.0
39	318467.0
40	2135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.116000802613136	22.21627693975078	25.439969861057836	21.227752396578246
2	25.302242539315078	24.04222181879234	27.957144826626006	22.698390815266574
3	25.329542305765575	25.439287366896572	26.780388393777294	22.45078193356056
4	25.503714815719754	28.485804803939903	23.72731901278585	22.2831613675545
5	23.301442656158077	35.12538100236552	21.164143940748588	20.40903240072781
6	86.96886598135153	2.697216925309204	7.419394027084099	2.914523066255168
7	86.5477670838526	2.191215754149223	5.638630261518113	5.622386900480066
8	76.22545239125479	3.996549309520657	12.5475186559779	7.230479643246652
9	53.56746523033495	15.37836793806229	15.051999230146587	16.00216760145617
10	47.253575245663775	17.41697799775323	23.918007881442573	11.411438875140423
11	43.55623137644057	15.92272528108522	23.606244548577887	16.914798793896317
12	18.97975312821199	38.25584522124414	27.07085790881059	15.69354374173329
13	12.268651541686062	41.355460704033675	28.552825730575876	17.823062023704388
14	10.632167041810957	21.235805827681144	51.92920624564057	16.20282088486733
15	12.253909667802793	15.55103896086169	52.015064011127386	20.179987360208134
16	11.755825428913456	19.946574357056377	28.56456463014959	39.733035583880586
17	17.815691086762754	22.334894424978195	36.829705421870116	23.019709066388938
18	24.970413878109273	26.958109873370034	27.592556445679605	20.478919802841087
19	26.66463738402718	22.040602942641826	25.79036236344998	25.504397309881018
20	16.797136800494673	32.9560050613767	27.839346334392108	22.40751180373652
21	18.654476410954306	24.96099545868385	24.876912178016315	31.50761595234553
22	15.358985103882436	32.4848110924411	22.261594552058607	29.894609251617855
23	15.734356892576784	23.200297021458983	39.89915466273186	21.166191423232377
24	15.910986381511504	21.982590938934518	37.398905552363	24.70751712719098
25	13.02512807002936	32.19625256105934	30.775845712639928	24.00277365627137
26	16.896917446871242	30.913300036718184	27.126549432369607	25.063233084040963
27	16.936502108224463	27.745025641305638	37.482579336533774	17.83589291393612
28	15.354890138914861	24.416911113325426	32.42789107939182	27.800307668367896
29	17.22956510107056	32.35308971931745	27.3621464168374	23.055198762774584
30	27.559660227106757	24.865855772603865	21.321527094335707	26.252956905953667
31	30.957389159535744	26.284078639707236	22.123184736154585	20.63534746460244
32	23.168083297047392	29.744733533804617	22.145161048147234	24.942022121000754
33	20.899745702675514	25.993745623506193	25.16246773508853	27.94404093872977
34	18.261632771731637	25.010271537127	30.70254583972034	26.02554985142102
35	33.187643579709174	22.085920554949652	23.080587545573547	21.645848319767623
36	22.058757287331407	28.237786425737127	30.043392978773063	19.6600633081584
37	21.590566292705365	27.72536980946128	26.514625167381695	24.169438730451663
38	18.429799333066704	26.474221513034955	29.821991872859527	25.273987281038814
39	24.164934268987327	22.107896866942305	27.77423639140767	25.9529324726627
40	14.678811422768279	19.556187696814252	32.87615324450899	32.888847635908476
41	22.098614946349134	24.391522330526463	24.226631741165455	29.28323098195895
42	21.990917367701922	21.37216816110138	27.90131680423474	28.735597666961958
43	24.44735035291773	26.936952554370897	27.59555941998916	21.02013767272221
44	15.80424429469006	33.76312265648567	25.991288644525646	24.441344404298622
45	23.190196107872296	28.107566539768253	26.238624528567158	22.463612823792293
46	23.319460502015403	21.352239331592518	28.914138139548218	26.41416202684386
47	22.268009997174474	18.627449642168312	32.08759949058636	27.016940870070854
48	21.610358623381977	16.907427856954683	30.656136236754495	30.82607728290884
49	20.814843429014466	24.283005758885736	30.366758712379216	24.53539209972059
50	19.61515519234733	25.96030340960433	27.304543909626855	27.11999748842149
51	19.190643824042088	20.223939984193436	32.83888906330406	27.746527128460418
52	22.023131092113506	18.352950490508555	34.97700677170707	24.646911645670873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	211.5
2	219.0
3	1225.5
4	2232.0
5	1583.5
6	935.0
7	1087.0
8	1239.0
9	1326.5
10	1414.0
11	1400.5
12	1387.0
13	1323.5
14	1190.5
15	1121.0
16	1126.0
17	1131.0
18	1004.5
19	878.0
20	964.0
21	1050.0
22	1104.0
23	1158.0
24	1211.0
25	1264.0
26	1528.5
27	1793.0
28	2499.0
29	3205.0
30	3513.0
31	3821.0
32	4768.0
33	5715.0
34	6702.0
35	7689.0
36	8800.0
37	9911.0
38	11115.5
39	15079.0
40	17838.0
41	28197.5
42	38557.0
43	55171.5
44	71786.0
45	83363.5
46	94941.0
47	100720.0
48	106499.0
49	102889.0
50	99279.0
51	87164.5
52	75050.0
53	61845.0
54	48640.0
55	40079.5
56	31519.0
57	27339.0
58	23159.0
59	21952.5
60	20746.0
61	18165.0
62	15584.0
63	13461.0
64	9417.5
65	7497.0
66	5885.0
67	4273.0
68	3278.0
69	2283.0
70	1766.0
71	1249.0
72	1145.0
73	1041.0
74	790.5
75	540.0
76	529.0
77	518.0
78	359.5
79	201.0
80	157.5
81	114.0
82	60.0
83	6.0
84	4.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	732607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.063808611934462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.89845817315344	15.659970443510481
2	12.98204975867859	5.98831022049546
3	5.406534513305185	3.7408583180606705
4	2.7355717929605046	2.5237081710818994
5	1.6505687785880356	1.9034201205094439
6	1.045662531909328	1.4470176305176532
7	0.6518111999393141	1.0523274136560983
8	0.5497396874016534	1.0143272749293128
9	0.42316087247652967	0.8783731237372097
>10	4.693445302269054	28.249678086281214
>50	1.6687554113485719	24.961830188566214
>100	0.28652999064392437	10.204822954348035
>500	0.004152608560056875	0.7214591598615967
>1k	0.0035593787657630356	1.6538968944447148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	3337	0.4554966032265594	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	2733	0.3730513085460554	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2374	0.32404822776741143	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1301	0.1775849807604896	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	1202	0.16407159636749308	No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1141	0.1557451676000912	No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	952	0.12994688830437057	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	893	0.12189345720147364	No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	794	0.10838007280847713	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	782	0.10674208682144724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37919375599741745	0.0
2	0.0	0.0	0.0	1.4483891090311722	0.0
3	0.0	0.0	0.0	2.3920055363926362	0.0
4	0.0	0.0	0.0	2.9865944496844827	0.0
5	0.0	0.0	0.0	6.051948725578653	0.0
6	0.0	0.0	0.0	6.686941293217236	0.0
7	0.0	0.0	0.0	10.055459475544186	0.0
8	0.0	0.0	0.0	13.426161639187177	0.0
9	0.0	0.0	0.0	16.7987747864817	0.0
10	0.0	0.0	0.0	18.05074207590154	0.0
11	0.0	0.0	0.0	19.311718288250045	0.0
12	0.0	0.0	0.0	20.42090779913378	0.0
13	0.0	0.0	0.0	20.77894423613206	0.0
14	0.0	0.0	0.0	21.066820273352562	0.0
15	0.0	0.0	0.0	22.087558540936683	0.0
16	0.0	0.0	0.0	23.203436494600787	0.0
17	0.0	0.0	0.0	24.998396138721034	0.0
18	0.0	0.0	0.0	25.606088939909117	0.0
19	0.0	0.0	0.0	26.505479745620775	0.0
20	0.0	0.0	0.0	27.486087356522663	0.0
21	0.0	0.0	0.0	28.27518710577431	0.0
22	1.3649883225249008E-4	0.0	0.0	29.246239798418525	0.0
23	1.3649883225249008E-4	0.0	0.0	29.853932599606612	0.0
24	1.3649883225249008E-4	0.0	0.0	30.35010585484441	0.0
25	1.3649883225249008E-4	0.0	0.0	30.87412487186172	0.0
26	1.3649883225249008E-4	0.0	0.0	31.327164496107734	0.0
27	1.3649883225249008E-4	0.0	0.0	31.849136030641258	0.0
28	1.3649883225249008E-4	0.0	0.0	32.28688778567499	0.0
29	1.3649883225249008E-4	0.0	0.0	32.971156431756725	0.0
30	1.3649883225249008E-4	0.0	0.0	33.60382851924702	0.0
31	1.3649883225249008E-4	0.0	0.0	34.07884445548569	0.0
32	2.7299766450498015E-4	0.0	0.0	34.57556370605249	0.0
33	4.0949649675747025E-4	0.0	0.0	35.05917906872307	0.0
34	4.0949649675747025E-4	0.0	0.0	35.76283054898465	0.0
35	4.0949649675747025E-4	0.0	0.0	36.24207794902315	0.0
36	4.0949649675747025E-4	0.0	0.0	36.69893954057223	0.0
37	4.0949649675747025E-4	0.0	0.0	37.14774770101842	0.0
38	4.0949649675747025E-4	0.0	0.0	37.56393264055626	0.0
39	4.0949649675747025E-4	0.0	0.0	38.0651563525874	0.0
40	4.0949649675747025E-4	0.0	0.0	38.55150169190303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	50	1.6370905E-11	46.0	5
AGTCCGG	20	6.3099485E-4	46.0	10
TCTTGCG	20	6.3099485E-4	46.0	21
TCGCAGG	50	1.6370905E-11	46.0	1
CGTATGA	20	6.3099485E-4	46.0	20
GTCGAGT	20	6.3099485E-4	46.0	13
CGAAACA	20	6.3099485E-4	46.0	34
CGTGCGG	20	6.3099485E-4	46.0	1
GCGCGAC	20	6.3099485E-4	46.0	8
TAGAAGT	25	3.4159348E-5	46.0	8
ACTCTGC	20	6.3099485E-4	46.0	38
ACTTCGA	20	6.3099485E-4	46.0	15
CGATTGA	20	6.3099485E-4	46.0	9
TGAGTCA	20	6.3099485E-4	46.0	12
CGCGAAG	20	6.3099485E-4	46.0	13
CGCGAAA	20	6.3099485E-4	46.0	17
ATCGTCT	25	3.4159348E-5	46.0	39
ATCCGAC	20	6.3099485E-4	46.0	19
ATCCGAA	20	6.3099485E-4	46.0	27
ATTCCGG	40	5.6061253E-9	46.0	1
>>END_MODULE