FastQCFastQC Report
Thu 26 May 2016
SRR1544899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544899_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences428772
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT26230.6117470357206162No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT21820.5088951703935892Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT16330.38085509314973925No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTTG12100.2822012631421828Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.19474219398654763No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC7030.16395660164376405No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT7030.16395660164376405No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC5900.13760226880486598No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC5470.12757362887501983No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT5000.11661209220751355No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC4750.11078148759713786No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4590.10704990064649743No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4510.10518410717117721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC750.046.0000044
GTGCACG206.3070055E-446.012
TCGTTTA253.4135475E-546.03
AGCAACG206.3070055E-446.09
GGGTGCG301.8586543E-646.06
TCGTTGA206.3070055E-446.023
ATCTCGG206.3070055E-446.01
GTACCGG206.3070055E-446.01
CCGGGCT206.3070055E-446.04
TTAATCG206.3070055E-446.019
CTCAACC206.3070055E-446.035
CACGGGT301.8586543E-646.03
GCAACGA206.3070055E-446.010
CTATACT206.3070055E-446.013
CGTTGAT206.3070055E-446.024
GCTATCC253.4135475E-546.08
ATAGCGG650.046.01
TAAACGG206.3070055E-446.01
TTCGTGG206.3070055E-446.01
CGTCTGG206.3070055E-446.032
Produced by FastQC (version 0.11.5)