##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544899_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	428772
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91815930144692	34.0	33.0	34.0	31.0	34.0
2	33.249165057419795	34.0	33.0	34.0	31.0	34.0
3	33.3627498997136	34.0	34.0	34.0	31.0	34.0
4	36.59822236526639	37.0	37.0	37.0	35.0	37.0
5	36.594803298722866	37.0	37.0	37.0	35.0	37.0
6	36.41110426986837	37.0	37.0	37.0	35.0	37.0
7	36.238576679447355	37.0	37.0	37.0	35.0	37.0
8	36.38774220331552	37.0	37.0	37.0	35.0	37.0
9	38.2526307688002	39.0	39.0	39.0	37.0	39.0
10	38.075116845316394	39.0	39.0	39.0	35.0	39.0
11	37.25036149748584	39.0	35.0	39.0	35.0	39.0
12	37.17329956247143	39.0	35.0	39.0	35.0	39.0
13	37.16507374548711	39.0	35.0	39.0	35.0	39.0
14	38.33407965072346	40.0	35.0	41.0	35.0	41.0
15	38.40166801936694	40.0	35.0	41.0	35.0	41.0
16	38.445437202056105	40.0	35.0	41.0	35.0	41.0
17	38.57295252488502	40.0	36.0	41.0	35.0	41.0
18	38.55879581689103	40.0	36.0	41.0	35.0	41.0
19	38.465697386956236	40.0	35.0	41.0	35.0	41.0
20	38.36216917149441	40.0	35.0	41.0	35.0	41.0
21	38.31613304973273	40.0	35.0	41.0	35.0	41.0
22	38.305955146324855	40.0	35.0	41.0	35.0	41.0
23	38.27939790844552	40.0	35.0	41.0	35.0	41.0
24	38.24613780750609	40.0	35.0	41.0	35.0	41.0
25	38.219690184993425	40.0	35.0	41.0	35.0	41.0
26	38.1651367160169	40.0	35.0	41.0	35.0	41.0
27	38.246699877790526	40.0	35.0	41.0	35.0	41.0
28	38.2849439795509	40.0	35.0	41.0	35.0	41.0
29	38.27904107544336	40.0	35.0	41.0	35.0	41.0
30	38.20555913165972	40.0	35.0	41.0	35.0	41.0
31	38.10580448350172	40.0	35.0	41.0	35.0	41.0
32	38.02868657468305	40.0	35.0	41.0	35.0	41.0
33	37.986155812413124	40.0	35.0	41.0	35.0	41.0
34	37.82826770404784	40.0	35.0	41.0	35.0	41.0
35	37.81133562825931	40.0	35.0	41.0	35.0	41.0
36	37.72381358857388	40.0	35.0	41.0	35.0	41.0
37	37.67069444833152	40.0	35.0	41.0	35.0	41.0
38	37.64401593387628	40.0	35.0	41.0	35.0	41.0
39	37.62830595281408	40.0	35.0	41.0	35.0	41.0
40	37.63787280885879	40.0	35.0	41.0	35.0	41.0
41	37.62243336785051	40.0	35.0	41.0	35.0	41.0
42	37.54449917438639	40.0	35.0	41.0	35.0	41.0
43	37.47456690268954	39.0	35.0	41.0	35.0	41.0
44	37.2592706613305	39.0	35.0	41.0	35.0	41.0
45	37.29899806890376	39.0	35.0	41.0	35.0	41.0
46	37.264135717817396	39.0	35.0	41.0	35.0	41.0
47	37.2328743481384	39.0	35.0	41.0	35.0	41.0
48	37.22573768809531	39.0	35.0	41.0	35.0	41.0
49	37.13257628763072	38.0	35.0	41.0	35.0	41.0
50	37.06692134747605	38.0	35.0	41.0	35.0	41.0
51	36.99710102338772	38.0	35.0	41.0	34.0	41.0
52	36.584205591783046	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	9.0
17	16.0
18	26.0
19	51.0
20	91.0
21	174.0
22	274.0
23	463.0
24	817.0
25	1396.0
26	1909.0
27	2075.0
28	2004.0
29	1982.0
30	2178.0
31	2842.0
32	4026.0
33	6227.0
34	20483.0
35	108684.0
36	12893.0
37	19287.0
38	37484.0
39	202380.0
40	996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.33710223615348	21.851473510397135	24.505098280671312	23.306325972778072
2	25.019824055675276	24.535650648829684	27.757176308154452	22.68734898734059
3	25.260044965622757	25.295961490022666	27.108813075480676	22.3351804688739
4	25.518923810323436	28.205199966415716	23.961919155168715	22.313957068092133
5	23.560307109606036	34.52510891569412	21.386191262489156	20.528392712210685
6	85.62755963542395	2.666452100417005	8.124597688281884	3.581390575877156
7	84.91552620040488	2.113710783353391	6.475469480283228	6.4952935359585044
8	74.06500424468015	4.060433050665622	13.830427359995523	8.0441353446587
9	52.337839224576236	15.052988534699093	15.850615245398487	16.758556995326188
10	45.653167650872724	17.02816415250996	25.648363232673777	11.670304963943542
11	42.038659240808634	15.813532600076496	24.236190796040784	17.911617363074082
12	18.34634724282369	36.582612670603496	28.55760170906682	16.51343837750599
13	12.116462828729487	39.64881102310785	29.285727612810536	18.94899853535212
14	10.40226507327904	20.8297183584749	51.34477064733704	17.423245920909014
15	11.94760851921301	15.622755217225007	51.5318164432379	20.897819820324088
16	11.53806685138022	20.385892735533105	29.323743154870186	38.75229725821649
17	17.365173098989672	22.438732006754172	37.2934799847005	22.90261490955566
18	24.100221096526823	27.652225425167686	27.95051915703451	20.297034321270978
19	25.919369734964036	22.241191122554643	25.8039237636786	26.03551537880272
20	16.290242833020812	33.78485535436083	27.376087990820295	22.548813821798063
21	18.2052466112526	25.263776552573397	24.720364202886383	31.810612633287622
22	15.145811760096276	33.1961975128973	22.07676807254205	29.58122265446438
23	15.483053930760404	23.460487158676408	39.27518587967498	21.781273030888208
24	16.097366432509585	22.652831808047168	36.383205992928644	24.866595766514603
25	13.068950397880458	31.676275503064566	30.981034209323372	24.273739889731605
26	17.354211562322167	29.566762755030645	26.980772998236823	26.09825268441036
27	17.659268795537024	27.177147761514277	36.98142602595318	18.182157416995512
28	16.119755954213428	24.40527832974168	31.866586437547227	27.608379278497665
29	17.84794716072878	31.994859738975496	26.804455514819065	23.352737585476664
30	27.430196001604585	24.231059863983656	20.66552853264672	27.67321560176504
31	30.997359902232425	26.17008573321019	21.515630684839497	21.316923679717892
32	22.363633819372534	29.379950183314207	22.110352355097813	26.146063642215445
33	21.074836976295096	25.436129224856103	25.247450859664344	28.24158293918446
34	18.692218708311177	24.612381405502227	30.738947505900573	25.956452380286027
35	33.13206086218317	21.896485777989234	22.857602641963563	22.11385071786404
36	22.186849887585943	27.95471719235398	29.976071198679016	19.882361721381063
37	21.94219771813458	27.068931739945707	26.974242721073203	24.01462782084651
38	18.26821714104466	25.883919658932953	30.017585103504896	25.830278096517496
39	24.0377170151036	21.53335572285504	28.19190618790406	26.237021074137303
40	14.70198613715448	18.76848301661489	33.062560055227486	33.46697079100314
41	22.199910441913183	24.20353941022268	24.186514044760386	29.41003610310375
42	21.604722323286037	21.114018639276818	28.190506842797568	29.090752194639574
43	25.250249549877324	26.263375406976202	27.627270437435282	20.85910460571119
44	15.921282173276241	32.33373447893052	27.145895720802667	24.599087626990567
45	22.944128814381536	27.476840838487586	26.596186318136443	22.982844028994432
46	23.440429878816712	20.30169880495928	29.49749517225938	26.760376143964628
47	22.190581474536582	17.67372869497075	32.31764201020589	27.818047820286772
48	21.248822217868703	16.25665855046505	30.307949213101605	32.186570018564645
49	20.518597296465256	23.119979849430468	30.932990027333872	25.428432826770404
50	19.892157137126492	24.919537656376818	27.831108374614015	27.35719683188268
51	18.989579543440335	19.23096657430989	33.37134887539298	28.408105006856793
52	21.893453863591837	17.747194313061488	35.42558749172054	24.933764331626133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	137.5
2	170.0
3	780.5
4	1391.0
5	979.5
6	568.0
7	654.0
8	740.0
9	808.5
10	877.0
11	853.5
12	830.0
13	834.0
14	783.5
15	729.0
16	708.5
17	688.0
18	590.5
19	493.0
20	538.5
21	584.0
22	637.5
23	691.0
24	688.5
25	686.0
26	830.5
27	975.0
28	1265.5
29	1556.0
30	1769.0
31	1982.0
32	2483.5
33	2985.0
34	3486.0
35	3987.0
36	4749.5
37	5512.0
38	6156.5
39	8127.5
40	9454.0
41	15261.0
42	21068.0
43	30030.5
44	38993.0
45	46352.0
46	53711.0
47	59034.5
48	64358.0
49	62527.5
50	60697.0
51	53531.5
52	46366.0
53	37972.5
54	29579.0
55	24298.0
56	19017.0
57	16275.5
58	13534.0
59	12770.0
60	12006.0
61	10490.5
62	8975.0
63	8045.0
64	5793.5
65	4472.0
66	3533.5
67	2595.0
68	2008.0
69	1421.0
70	1114.0
71	807.0
72	705.5
73	604.0
74	467.5
75	331.0
76	316.0
77	301.0
78	212.5
79	124.0
80	86.0
81	48.0
82	27.0
83	6.0
84	3.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	428772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.29655275107684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.60815971735825	19.97967516098686
2	12.000216526374992	6.791295199258281
3	4.537391862092304	3.8517764453400494
4	2.0863977598589796	2.3615145708631267
5	1.199107881999961	1.6965309718621961
6	0.8223801055765216	1.3962313223329315
7	0.590053031927543	1.1687526720709382
8	0.46855712372424735	1.0606841094684802
9	0.41483945937023836	1.0564673980768333
>10	6.719227571640649	44.943725896519595
>50	0.4478933975542953	8.508308327294104
>100	0.09833821828221612	4.606776847029658
>500	0.004131857911017484	0.789893517550692
>1k	0.003305486328813987	1.7883675613462677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2623	0.6117470357206162	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	2182	0.5088951703935892	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	1633	0.38085509314973925	No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTTG	1210	0.2822012631421828	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	835	0.19474219398654763	No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	703	0.16395660164376405	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	703	0.16395660164376405	No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	590	0.13760226880486598	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	547	0.12757362887501983	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	500	0.11661209220751355	No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	475	0.11078148759713786	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	459	0.10704990064649743	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	451	0.10518410717117721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5179909135857752	0.0
2	0.0	0.0	0.0	1.8135512580112507	0.0
3	0.0	0.0	0.0	2.84067056617503	0.0
4	0.0	0.0	0.0	3.5158545800565335	0.0
5	0.0	0.0	0.0	6.849094623716101	0.0
6	0.0	0.0	0.0	7.538971761215751	0.0
7	0.0	0.0	0.0	11.382972768744228	0.0
8	0.0	0.0	0.0	15.141613724776805	0.0
9	0.0	0.0	0.0	19.06211226479341	0.0
10	0.0	0.0	0.0	20.502271603556203	0.0
11	0.0	0.0	0.0	21.869431772597093	0.0
12	0.0	0.0	0.0	23.081964307370818	0.0
13	0.0	0.0	0.0	23.48101088690493	0.0
14	0.0	0.0	0.0	23.783269429906802	0.0
15	0.0	0.0	0.0	24.89271687516909	0.0
16	0.0	0.0	0.0	26.03038444674559	0.0
17	0.0	0.0	0.0	27.780498726595955	0.0
18	0.0	0.0	0.0	28.395277676713963	0.0
19	0.0	0.0	0.0	29.289225975576763	0.0
20	0.0	0.0	0.0	30.26060470366535	0.0
21	0.0	0.0	0.0	31.058231414364744	0.0
22	0.0	0.0	0.0	32.04640228373121	0.0
23	0.0	0.0	0.0	32.6530183873947	0.0
24	0.0	0.0	0.0	33.168210610767495	0.0
25	0.0	0.0	0.0	33.70649202839738	0.0
26	0.0	0.0	0.0	34.179237450206635	0.0
27	0.0	0.0	0.0	34.71192148741056	0.0
28	0.0	0.0	0.0	35.14945005737315	0.0
29	0.0	0.0	0.0	35.76119709309376	0.0
30	0.0	0.0	0.0	36.364314833991024	0.0
31	0.0	0.0	0.0	36.855251742184656	0.0
32	0.0	0.0	0.0	37.36857817208213	0.0
33	0.0	0.0	0.0	37.84948644034592	0.0
34	0.0	0.0	0.0	38.52257143656769	0.0
35	0.0	0.0	0.0	39.01000998199509	0.0
36	0.0	0.0	0.0	39.47132741876802	0.0
37	0.0	0.0	0.0	39.8885654846865	0.0
38	0.0	0.0	0.0	40.295774910675135	0.0
39	0.0	0.0	0.0	40.79557433787654	0.0
40	0.0	0.0	0.0	41.254093084436484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGTC	75	0.0	46.000004	4
GTGCACG	20	6.3070055E-4	46.0	12
TCGTTTA	25	3.4135475E-5	46.0	3
AGCAACG	20	6.3070055E-4	46.0	9
GGGTGCG	30	1.8586543E-6	46.0	6
TCGTTGA	20	6.3070055E-4	46.0	23
ATCTCGG	20	6.3070055E-4	46.0	1
GTACCGG	20	6.3070055E-4	46.0	1
CCGGGCT	20	6.3070055E-4	46.0	4
TTAATCG	20	6.3070055E-4	46.0	19
CTCAACC	20	6.3070055E-4	46.0	35
CACGGGT	30	1.8586543E-6	46.0	3
GCAACGA	20	6.3070055E-4	46.0	10
CTATACT	20	6.3070055E-4	46.0	13
CGTTGAT	20	6.3070055E-4	46.0	24
GCTATCC	25	3.4135475E-5	46.0	8
ATAGCGG	65	0.0	46.0	1
TAAACGG	20	6.3070055E-4	46.0	1
TTCGTGG	20	6.3070055E-4	46.0	1
CGTCTGG	20	6.3070055E-4	46.0	32
>>END_MODULE