FastQCFastQC Report
Thu 26 May 2016
SRR1544898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544898_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences647891
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT29800.45995391200062974TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT18660.2880114093265688Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT18540.28615924592253944TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1879945855089822No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8050.12424929502030434No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT7560.11668629445385104TruSeq Adapter, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC7060.10896894693706194No Hit
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG6530.1007885585692655No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC206.309406E-446.031
CCTTCGG206.309406E-446.023
GGTACGT301.8601368E-646.07
CAAACGA301.8601368E-646.028
TCACCGA206.309406E-446.040
GGTCGTC253.4154928E-546.07
CTCCGCG206.309406E-446.019
ACTTGCA206.309406E-446.09
CTATAGG800.046.01
CGTTGAT206.309406E-446.024
GGGTACG600.046.06
ATAGCGG900.046.01
TAAACGG253.4154928E-546.01
CCGGAAC206.309406E-446.013
CCTACGG253.4154928E-546.01
CACGACG206.309406E-446.025
CCGCTTG206.309406E-446.032
TACGGGT850.046.03
CCGCTCG206.309406E-446.015
TTATCGG206.309406E-446.01
Produced by FastQC (version 0.11.5)