FastQCFastQC Report
Thu 26 May 2016
SRR1544897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544897_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences410885
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT23080.5617143482969688TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT15870.3862394587293282TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT15010.36530902807354854Illumina PCR Primer Index 8 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTTG10140.24678438005768036TruSeq Adapter, Index 14 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.1754748895676406No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6130.1491901626975918No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6060.1474865229930516No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT5660.1377514389671076TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC5230.12728622363921777TruSeq Adapter, Index 14 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4840.11779451671392238No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC4690.1141438602041934No Hit
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG4640.1129269747009504No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC4560.11097995789576158No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT4360.10611241588278958Illumina PCR Primer Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG351.0174335E-746.0000041
GGGTCAA351.0174335E-746.0000046
TACGGGA351.0174335E-746.0000043
AATGCGG351.0174335E-746.0000041
AAGTAGG405.5970304E-946.01
CGGGTAC600.046.05
GCGACGG301.8584633E-646.01
GATCGTT206.3066976E-446.09
GGTAATC206.3066976E-446.07
GATAAGG301.8584633E-646.01
GATCGCC253.4132965E-546.020
CGCGGTA206.3066976E-446.030
TAGACCG206.3066976E-446.045
TAGGTTC253.4132965E-546.028
GATCCCA253.4132965E-546.08
CAACCGG551.8189894E-1246.01
GGATCGT206.3066976E-446.08
CGACCAA206.3066976E-446.028
TACGGGT253.4132965E-546.03
TCGATGG206.3066976E-446.01
Produced by FastQC (version 0.11.5)