FastQCFastQC Report
Thu 26 May 2016
SRR1544896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544896_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences656404
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT33040.5033485475408437No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT28870.4398205982900774Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT26350.4014296073759453No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.22486151821134542No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC10610.16163825936465956No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT10370.1579819745156946No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC8860.13497784900762336No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC8700.13254032577498004No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT8680.13223563537089963No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC8550.13025514774437694No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT7630.1162393891566779No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG7020.10694633183222528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1500.046.0000041
CTTCGAT206.3094666E-446.013
CGGGTCA253.415544E-546.05
AAGTAGG1200.046.01
TCGTTGA206.3094666E-446.023
CAAACGT206.3094666E-446.044
CAAACGG405.6043064E-946.01
ACGTTTG206.3094666E-446.032
TTTACGG301.860175E-646.01
CAACGCA253.415544E-546.016
CAACGAG206.3094666E-446.021
CATGCGG1100.046.01
CCGATGT253.415544E-546.011
GGGTACG253.415544E-546.06
GCTATCG206.3094666E-446.011
TGAACGA253.415544E-546.027
TGTATCG206.3094666E-446.012
TTCAACG206.3094666E-446.019
TACGGGT253.415544E-546.03
TAACGCA253.415544E-546.024
Produced by FastQC (version 0.11.5)