##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544896_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	656404
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.830611940207554	34.0	31.0	34.0	31.0	34.0
2	33.12317261930153	34.0	33.0	34.0	31.0	34.0
3	33.17492123753055	34.0	33.0	34.0	31.0	34.0
4	36.491720038269115	37.0	37.0	37.0	35.0	37.0
5	36.47262204374135	37.0	37.0	37.0	35.0	37.0
6	36.61354135562855	37.0	37.0	37.0	35.0	37.0
7	36.42691238932121	37.0	37.0	37.0	35.0	37.0
8	36.47661958184289	37.0	37.0	37.0	35.0	37.0
9	38.24885588753268	39.0	39.0	39.0	37.0	39.0
10	38.00886496730672	39.0	39.0	39.0	35.0	39.0
11	37.17050017976734	39.0	35.0	39.0	35.0	39.0
12	37.061660197073756	39.0	35.0	39.0	35.0	39.0
13	37.06456999043272	39.0	35.0	39.0	35.0	39.0
14	38.31226500752585	40.0	35.0	41.0	35.0	41.0
15	38.374118683006195	40.0	35.0	41.0	35.0	41.0
16	38.39890524737814	40.0	35.0	41.0	35.0	41.0
17	38.4893739221577	40.0	36.0	41.0	35.0	41.0
18	38.461109316823176	40.0	36.0	41.0	35.0	41.0
19	38.361690361423754	40.0	35.0	41.0	35.0	41.0
20	38.272822834717644	40.0	35.0	41.0	35.0	41.0
21	38.2465798502142	40.0	35.0	41.0	35.0	41.0
22	38.21057610861604	40.0	35.0	41.0	35.0	41.0
23	38.195295884851404	40.0	35.0	41.0	35.0	41.0
24	38.13813139468986	40.0	35.0	41.0	35.0	41.0
25	38.114712890232234	40.0	35.0	41.0	35.0	41.0
26	38.07613756162363	40.0	35.0	41.0	35.0	41.0
27	38.053410704383275	40.0	35.0	41.0	35.0	41.0
28	38.078652780909316	40.0	35.0	41.0	35.0	41.0
29	38.09608564237878	40.0	35.0	41.0	35.0	41.0
30	37.994535377602816	40.0	35.0	41.0	35.0	41.0
31	37.92518479473007	40.0	35.0	41.0	35.0	41.0
32	37.85001005478333	40.0	35.0	41.0	35.0	41.0
33	37.72308517315555	40.0	35.0	41.0	35.0	41.0
34	37.6465576078147	40.0	35.0	41.0	35.0	41.0
35	37.53712804918922	40.0	35.0	41.0	35.0	41.0
36	37.46994990889757	40.0	35.0	41.0	34.0	41.0
37	37.36695388815425	39.0	35.0	41.0	34.0	41.0
38	37.37930451368364	39.0	35.0	41.0	35.0	41.0
39	37.248983857502395	39.0	35.0	41.0	34.0	41.0
40	37.15314044399486	39.0	35.0	41.0	34.0	41.0
41	37.12828989463806	39.0	35.0	41.0	34.0	41.0
42	37.0268889281601	39.0	35.0	41.0	34.0	41.0
43	36.91504926843834	38.0	35.0	41.0	34.0	41.0
44	36.84763346963151	38.0	35.0	41.0	34.0	41.0
45	36.825633299004885	38.0	35.0	41.0	34.0	41.0
46	36.89255245245306	38.0	35.0	41.0	34.0	41.0
47	36.8061605352801	38.0	35.0	41.0	34.0	41.0
48	36.832345628606774	38.0	35.0	41.0	34.0	41.0
49	36.708310735461694	38.0	35.0	41.0	34.0	41.0
50	36.54934613439284	37.0	35.0	40.0	34.0	41.0
51	36.342089627729266	37.0	35.0	40.0	33.0	41.0
52	36.03868958751013	36.0	35.0	39.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	8.0
15	11.0
16	11.0
17	22.0
18	56.0
19	133.0
20	194.0
21	355.0
22	571.0
23	944.0
24	1469.0
25	2555.0
26	3599.0
27	4031.0
28	4209.0
29	4470.0
30	5272.0
31	6479.0
32	8478.0
33	12021.0
34	34121.0
35	159865.0
36	22132.0
37	31432.0
38	59907.0
39	292083.0
40	1973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.539423891383965	23.146720617180883	25.64350613341783	20.67034935801732
2	26.096123728679288	23.891383964753416	27.012937154557253	22.999555152010043
3	24.669715601976833	25.605876868513906	27.742365981925765	21.9820415475835
4	26.42869330473306	27.456414037696298	23.93465000213283	22.18024265543781
5	23.082735632323995	35.560417060225106	20.553500588052483	20.80334671939842
6	86.13719599514933	2.6210992011017606	8.308145593262687	2.9335592104862247
7	86.22189992748368	2.037007696479607	5.641647521952944	6.099444854083766
8	75.55834516547736	3.9687448583494307	12.909427730483056	7.563482245690155
9	51.73338370881348	18.44930865747314	14.794547260528576	15.022760373184807
10	44.04467370704627	17.99120663493824	27.354190407127316	10.609929250888172
11	41.452215403928065	15.979031206391186	24.2797118847539	18.289041504926846
12	18.705857977708853	36.219614749453086	29.33620148567041	15.738325787167659
13	12.14023680538205	39.738027190571664	28.515365537077773	19.606370466968514
14	10.04122461167208	22.017080944052747	50.3266281131742	17.615066331100966
15	10.957581001943925	18.051535334946163	50.18007202881153	20.81081163429839
16	12.30111333873651	21.235245367182408	29.332088165215325	37.13155312886576
17	16.321503220577572	22.654340924186933	37.3347816283874	23.689374226848102
18	22.26845052741909	28.39470813706193	28.244648113052328	21.09219322246665
19	25.061090426018122	22.823901134057685	26.439357468875873	25.67565097104832
20	16.1662634596986	34.66965466389602	27.354190407127316	21.809891469278067
21	18.64720507492337	25.143509180321878	24.70582141486036	31.503464329894392
22	14.534189310241864	32.62914302776949	22.234325202162083	30.60234245982657
23	14.481173179931872	22.68739983302966	40.16794535072912	22.663481636309346
24	15.851061236677413	23.129200918946257	36.13003577065344	24.88970207372289
25	12.94995155422575	31.708063936234392	30.655510935338604	24.686473574201255
26	17.122381947702937	31.00742835205148	26.97454616364312	24.895643536602456
27	17.877252423812166	28.41527473933736	36.62348188006167	17.08399095678881
28	14.466852730940092	25.81489448571307	31.9062650440887	27.811987739258136
29	19.53019786594841	30.162064825930372	26.757758941139908	23.54997836698131
30	25.3538979043394	28.362410954229407	21.07756808307079	25.2061230583604
31	30.2690111577626	26.737039993662435	21.16501422904187	21.82893461953309
32	24.725016910317425	29.863772920335645	23.069938635352617	22.34127153399431
33	19.032638436085094	30.063649825412398	23.18160766844809	27.722104070054414
34	15.57805863462136	31.452123996806847	29.49098421094326	23.478833157628536
35	25.95840366603494	24.13787850165447	25.026051029548874	24.877666802761713
36	21.579088488187153	29.258962468236028	27.011566047738896	22.150382995837926
37	16.666108067592518	31.046581068975815	24.69150096586858	27.595809897563083
38	18.58809513653177	30.707460649234314	24.294946404957923	26.409497809275994
39	28.034259389034798	21.52409187025064	24.09446011907301	26.34718862164155
40	18.187579600368068	20.000182814242446	29.524043119785986	32.288194465603496
41	22.94592964089189	24.24817642793158	23.7935783450436	29.01231558613293
42	21.587010438693245	22.782920274708868	27.85571081224368	27.77435847435421
43	23.2475731409315	27.910402739776114	27.08057842426311	21.76144569502928
44	14.712128506224825	33.13934710940213	27.36272173844157	24.78580264593147
45	21.31888288310248	29.4794059755882	24.810025533055864	24.391685608253454
46	23.485079310912184	23.778953205647742	26.623238127738404	26.11272935570167
47	21.38515304598997	19.809141930884028	30.801000603286997	28.004704419839
48	21.89383367560222	18.378315793322404	27.703060919799395	32.024789611275985
49	20.989664901493594	23.768898422313086	30.44359875930067	24.797837916892647
50	19.225964497474116	25.38741384878825	27.064277487644805	28.322344166092833
51	18.236634755425012	20.708283313325328	32.19983424842018	28.85524768282948
52	20.706759861304928	17.618722615949935	36.153192241363556	25.52132528138159
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	197.5
2	246.0
3	1304.0
4	2362.0
5	1637.5
6	913.0
7	1056.5
8	1200.0
9	1304.0
10	1408.0
11	1356.5
12	1305.0
13	1276.5
14	1213.5
15	1179.0
16	1120.5
17	1062.0
18	1025.5
19	989.0
20	1037.5
21	1086.0
22	1060.0
23	1034.0
24	1035.0
25	1036.0
26	1329.5
27	1623.0
28	2491.5
29	3360.0
30	3598.5
31	3837.0
32	4116.5
33	4396.0
34	5460.0
35	6524.0
36	7852.0
37	9180.0
38	10216.0
39	13697.0
40	16142.0
41	25672.0
42	35202.0
43	48050.5
44	60899.0
45	73559.5
46	86220.0
47	93194.0
48	100168.0
49	98256.5
50	96345.0
51	82314.0
52	68283.0
53	56949.0
54	45615.0
55	35842.5
56	26070.0
57	22239.5
58	18409.0
59	16555.0
60	14701.0
61	13701.0
62	12701.0
63	10170.0
64	6303.0
65	4967.0
66	3881.0
67	2795.0
68	1999.5
69	1204.0
70	1291.0
71	1378.0
72	1187.0
73	996.0
74	749.0
75	502.0
76	549.5
77	597.0
78	371.5
79	146.0
80	81.0
81	16.0
82	14.0
83	12.0
84	7.0
85	2.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	656404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.657849031307727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3048329823952	17.78212941802293
2	12.885269038734206	6.612165754472518
3	5.138346192887731	3.955167326631247
4	2.5116524019526234	2.5777439259368737
5	1.4475391676446743	1.8570370715165954
6	0.911009863237437	1.4024732122187076
7	0.6283224840561217	1.128498240722176
8	0.45066230241751	0.9250420255624017
9	0.3636504316601355	0.839743908813508
>10	5.126956103173836	35.157951200171176
>50	1.0141799750578697	16.63358654623528
>100	0.2092563606215533	8.323580873426735
>500	0.004755826377762575	0.9135042303638028
>1k	0.003566869783321931	1.89137626590602
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	3304	0.5033485475408437	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	2887	0.4398205982900774	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	2635	0.4014296073759453	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1476	0.22486151821134542	No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1061	0.16163825936465956	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1037	0.1579819745156946	No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	886	0.13497784900762336	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	870	0.13254032577498004	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	868	0.13223563537089963	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	855	0.13025514774437694	No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	763	0.1162393891566779	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	702	0.10694633183222528	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4477425487961682	0.0
2	0.0	0.0	0.0	1.6502032284995216	0.0
3	0.0	0.0	0.0	2.6706113917648278	0.0
4	0.0	0.0	0.0	3.269328035782841	0.0
5	0.0	0.0	0.0	6.672262813754943	0.0
6	0.0	0.0	0.0	7.389046989354117	0.0
7	0.0	0.0	0.0	11.217786607028598	0.0
8	0.0	0.0	0.0	15.028549490862336	0.0
9	0.0	0.0	0.0	19.048482337097276	0.0
10	0.0	0.0	0.0	20.43055801000603	0.0
11	0.0	0.0	0.0	21.854528613475846	0.0
12	0.0	0.0	0.0	23.006867721707973	0.0
13	0.0	0.0	0.0	23.438766369491958	0.0
14	0.0	0.0	0.0	23.789007988982394	0.0
15	0.0	0.0	0.0	24.949878428528773	0.0
16	0.0	0.0	0.0	26.137104588028105	0.0
17	0.0	0.0	0.0	28.174264629709754	0.0
18	0.0	0.0	0.0	28.875204904296744	0.0
19	0.0	0.0	0.0	29.890585675894723	0.0
20	0.0	0.0	0.0	30.89636869976417	0.0
21	0.0	0.0	0.0	31.694048177646692	0.0
22	0.0	0.0	0.0	32.67027623232034	0.0
23	0.0	0.0	0.0	33.31820037659734	0.0
24	0.0	0.0	0.0	33.865729032729845	0.0
25	0.0	0.0	0.0	34.45271509619076	0.0
26	0.0	0.0	0.0	34.9639855942377	0.0
27	0.0	0.0	0.0	35.45225196677656	0.0
28	0.0	0.0	0.0	35.92848306835425	0.0
29	0.0	0.0	0.0	36.67604097476554	0.0
30	0.0	0.0	0.0	37.34742628015673	0.0
31	0.0	0.0	0.0	37.84041535395884	0.0
32	0.0	0.0	0.0	38.312533135081445	0.0
33	0.0	0.0	0.0	38.809483184136596	0.0
34	0.0	0.0	0.0	39.54393940317244	0.0
35	0.0	0.0	0.0	40.05262003278469	0.0
36	0.0	0.0	0.0	40.53981998890927	0.0
37	0.0	0.0	0.0	41.00508223594006	0.0
38	0.0	0.0	0.0	41.457852176403556	0.0
39	0.0	0.0	0.0	41.94627089414446	0.0
40	0.0	0.0	0.0	42.45464683335263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	150	0.0	46.000004	1
CTTCGAT	20	6.3094666E-4	46.0	13
CGGGTCA	25	3.415544E-5	46.0	5
AAGTAGG	120	0.0	46.0	1
TCGTTGA	20	6.3094666E-4	46.0	23
CAAACGT	20	6.3094666E-4	46.0	44
CAAACGG	40	5.6043064E-9	46.0	1
ACGTTTG	20	6.3094666E-4	46.0	32
TTTACGG	30	1.860175E-6	46.0	1
CAACGCA	25	3.415544E-5	46.0	16
CAACGAG	20	6.3094666E-4	46.0	21
CATGCGG	110	0.0	46.0	1
CCGATGT	25	3.415544E-5	46.0	11
GGGTACG	25	3.415544E-5	46.0	6
GCTATCG	20	6.3094666E-4	46.0	11
TGAACGA	25	3.415544E-5	46.0	27
TGTATCG	20	6.3094666E-4	46.0	12
TTCAACG	20	6.3094666E-4	46.0	19
TACGGGT	25	3.415544E-5	46.0	3
TAACGCA	25	3.415544E-5	46.0	24
>>END_MODULE