FastQCFastQC Report
Thu 26 May 2016
SRR1544895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544895_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences428137
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT29780.6955717445584008No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT23120.5400140609197523Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT19900.4648044901515169No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTTG13250.30948037660842204Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.22703013287802737No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC8470.1978338709338366No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT8300.19386317930942665No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC6710.15672553411641602No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC6700.15649196402086246No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC5930.13850706666324097No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT5470.1277628422677788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG750.046.0000041
AGCGTGG253.4135395E-546.01
CGGGTCC206.3069945E-446.05
CGGGTAT453.092282E-1046.05
AGCCGTG206.3069945E-446.014
CAAACGG206.3069945E-446.01
ACCGGGT206.3069945E-446.03
CCGGGAT206.3069945E-446.04
CATGCGG1600.046.01
CCGGCTT253.4135395E-546.04
ATAATAC206.3069945E-446.010
TATGGGT301.858647E-646.03
TATGGGA1150.046.03
CACGCTT206.3069945E-446.022
ATAGCGG850.046.01
GCAGTGC301.858647E-646.038
CAACCGG301.858647E-646.01
TGAACGA301.858647E-646.027
CGACCCC301.858647E-646.019
TACGGGT206.3069945E-446.03
Produced by FastQC (version 0.11.5)