##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544895_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	428137
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8348425854341	34.0	33.0	34.0	31.0	34.0
2	33.17346550286474	34.0	33.0	34.0	31.0	34.0
3	33.303321133188675	34.0	34.0	34.0	31.0	34.0
4	36.561264268213215	37.0	37.0	37.0	35.0	37.0
5	36.546061190693635	37.0	37.0	37.0	35.0	37.0
6	36.35606359646562	37.0	37.0	37.0	35.0	37.0
7	36.16156277079533	37.0	37.0	37.0	35.0	37.0
8	36.33199653381978	37.0	37.0	37.0	35.0	37.0
9	38.201036116943875	39.0	39.0	39.0	37.0	39.0
10	38.03591840929422	39.0	39.0	39.0	35.0	39.0
11	37.32906522912059	39.0	35.0	39.0	35.0	39.0
12	37.28820214090349	39.0	35.0	39.0	35.0	39.0
13	37.29807047744063	39.0	35.0	39.0	35.0	39.0
14	38.53002426793293	41.0	35.0	41.0	35.0	41.0
15	38.55302157954113	41.0	35.0	41.0	35.0	41.0
16	38.609265258550415	41.0	35.0	41.0	35.0	41.0
17	38.711578303206686	41.0	36.0	41.0	35.0	41.0
18	38.69898887505635	41.0	36.0	41.0	35.0	41.0
19	38.63272503894781	41.0	35.0	41.0	35.0	41.0
20	38.5524610113118	41.0	35.0	41.0	35.0	41.0
21	38.533822117686626	41.0	35.0	41.0	35.0	41.0
22	38.496847971560506	40.0	35.0	41.0	35.0	41.0
23	38.45465820520067	40.0	35.0	41.0	35.0	41.0
24	38.42188131369164	40.0	35.0	41.0	35.0	41.0
25	38.38438163485053	40.0	35.0	41.0	35.0	41.0
26	38.338592553318215	40.0	35.0	41.0	35.0	41.0
27	38.41241471771886	40.0	35.0	41.0	35.0	41.0
28	38.41096658312643	40.0	36.0	41.0	35.0	41.0
29	38.43448709174867	40.0	36.0	41.0	35.0	41.0
30	38.33451909085176	40.0	35.0	41.0	35.0	41.0
31	38.26905406447002	40.0	35.0	41.0	35.0	41.0
32	38.208328175327054	40.0	35.0	41.0	35.0	41.0
33	38.144449089894124	40.0	35.0	41.0	35.0	41.0
34	38.034388525168346	40.0	35.0	41.0	35.0	41.0
35	37.980854259267474	40.0	35.0	41.0	35.0	41.0
36	37.928462151133864	40.0	35.0	41.0	35.0	41.0
37	37.847331578443345	40.0	35.0	41.0	35.0	41.0
38	37.86466247953342	40.0	35.0	41.0	35.0	41.0
39	37.80291355337194	40.0	35.0	41.0	35.0	41.0
40	37.7483165435363	40.0	35.0	41.0	35.0	41.0
41	37.72711305026195	40.0	35.0	41.0	35.0	41.0
42	37.64901421741172	40.0	35.0	41.0	35.0	41.0
43	37.562301319437466	40.0	35.0	41.0	35.0	41.0
44	37.41865337497109	39.0	35.0	41.0	35.0	41.0
45	37.43777108729215	39.0	35.0	41.0	35.0	41.0
46	37.38830094105391	39.0	35.0	41.0	35.0	41.0
47	37.292464795147346	39.0	35.0	41.0	35.0	41.0
48	37.24802574876733	39.0	35.0	41.0	35.0	41.0
49	37.12954965349876	39.0	35.0	41.0	35.0	41.0
50	36.96496682136792	38.0	35.0	41.0	35.0	41.0
51	36.71122327666144	37.0	35.0	40.0	34.0	41.0
52	36.138429988531705	37.0	35.0	39.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	3.0
16	2.0
17	16.0
18	40.0
19	63.0
20	115.0
21	195.0
22	337.0
23	476.0
24	850.0
25	1327.0
26	1978.0
27	2208.0
28	2142.0
29	2094.0
30	2506.0
31	3146.0
32	4130.0
33	6135.0
34	19154.0
35	97532.0
36	12900.0
37	19071.0
38	39472.0
39	211565.0
40	676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.775982921354615	22.900146448449913	24.782020708324673	22.541849921870803
2	25.798517764173617	24.216313936894032	26.996732354363207	22.988435944569144
3	24.460161116651914	25.574991182728894	27.783863576378586	22.180984124240606
4	26.435696984843638	27.00350588713426	24.254619432564812	22.306177695457297
5	23.10662241291923	35.121000988001505	20.736820223433153	21.035556375646113
6	84.49374849639251	2.5024700037604783	9.22671948465094	3.7770620151960705
7	84.30502385918525	1.8631886522304777	6.593450227380488	7.238337261203774
8	72.73559631613246	3.9826036992831737	14.691091870125684	8.590708114458689
9	49.65910444553963	17.979992385614885	15.931115507419355	16.429787661426133
10	41.7707416084104	17.509115072978975	29.74118097711714	10.978962341493494
11	39.37267743736234	15.71553030922345	25.264576525738256	19.647215727675952
12	18.146294293648996	33.70720119961601	31.57353837673455	16.572966130000445
13	11.997561528202421	37.28572863359159	29.884826585882557	20.83188325232344
14	10.002639342079755	21.665027783162866	49.418994387310605	18.913338487446776
15	10.839053854256932	17.932577656217518	49.72100052086132	21.507367968664237
16	12.063895435339623	21.965632496140255	30.40965859059133	35.5608134779288
17	15.450428250770198	22.770047905226598	37.8304140964224	23.9491097475808
18	21.33008826613911	29.36560026346707	28.81437483796075	20.489936632433075
19	23.704094717345146	23.04355848711978	26.654085024186188	26.59826177134889
20	15.38502862401521	35.76145019000928	26.777643604733996	22.075877581241517
21	17.72797025251263	25.3568367134819	24.835274690110875	32.07991834389459
22	14.351948091381963	33.254542354433276	22.058593394170558	30.334916160014203
23	13.876165806739433	23.081630412695002	39.46376977462822	23.57843400593735
24	16.470895998243552	23.288573517355427	35.012624463664665	25.227906020736352
25	12.85780019012606	31.150542933687113	31.274802224521594	24.716854651665237
26	17.736845916143665	29.214947551835042	26.91288069005949	26.135325841961805
27	18.845369589640697	27.746959501281133	36.18094208162341	17.226728827454764
28	15.210084622445619	25.89474864354167	30.780100762139224	28.115065971873488
29	20.442755473131264	29.581886171949634	25.927214886823613	24.048143468095493
30	24.914922092694628	27.65610073411081	20.346758163858762	27.082219009335795
31	30.85764603386299	26.585415416093443	20.118793750598524	22.438144799445038
32	24.338938237059633	28.89472295083116	23.401621443603332	23.364717368505875
33	19.419484884511267	29.652891480997905	22.73898308251798	28.18864055197285
34	15.907758497864002	31.714381144353325	29.592863966440646	22.784996391342023
35	24.590493229970782	24.3566895643217	25.006715140247167	26.046102065460353
36	21.78601709265959	29.27170508505455	26.251176609356353	22.691101212929507
37	16.39335072651978	30.761181584399388	24.809348409504434	28.0361192795764
38	19.049509853154483	30.400315786769188	23.249333741302433	27.300840618773897
39	28.482938872370294	20.713229643782245	24.156286422336777	26.647545061510687
40	18.94533759053761	19.173302003797847	29.096761083484957	32.78459932217958
41	23.190707647318497	24.032961411884514	23.54573419255986	29.23059674823713
42	21.485412379682202	21.855620981134543	28.192844813692812	28.466121825490436
43	24.359258835372792	26.92409205464606	27.17658132793942	21.54006778204173
44	14.901304956123857	31.24350383171742	28.734026725090335	25.12116448706839
45	20.94796758981354	28.72585177174596	25.150594319108137	25.175586319332368
46	23.9757367384739	21.853752420370114	27.32606618909368	26.844444652062304
47	21.201858283680224	18.431016240128745	31.524255086572754	28.842870389618273
48	21.487514510542187	17.419657726381978	27.49120024665002	33.60162751642582
49	20.703186129673444	22.2237274517269	31.229256055888655	25.843830362711
50	19.74414731733067	23.666723502056584	28.19774978569944	28.39137939491331
51	17.978590965041562	19.22165101357743	32.86377958457223	29.93597843680878
52	20.440419772175726	16.6164101677734	37.06126777176465	25.881902288286224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	113.5
2	153.0
3	839.0
4	1525.0
5	1088.0
6	651.0
7	712.5
8	774.0
9	868.5
10	963.0
11	917.0
12	871.0
13	856.0
14	804.5
15	768.0
16	703.5
17	639.0
18	613.0
19	587.0
20	632.0
21	677.0
22	680.5
23	684.0
24	682.0
25	680.0
26	799.5
27	919.0
28	1361.0
29	1803.0
30	1915.5
31	2028.0
32	2279.5
33	2531.0
34	3099.0
35	3667.0
36	4490.5
37	5314.0
38	5993.0
39	7990.0
40	9308.0
41	14978.5
42	20649.0
43	28481.0
44	36313.0
45	45118.5
46	53924.0
47	61014.5
48	68105.0
49	67626.0
50	67147.0
51	57763.5
52	48380.0
53	39801.5
54	31223.0
55	24334.0
56	17445.0
57	14544.0
58	11643.0
59	10598.5
60	9554.0
61	8885.5
62	8217.0
63	6681.5
64	4176.5
65	3207.0
66	2519.5
67	1832.0
68	1298.5
69	765.0
70	826.0
71	887.0
72	788.0
73	689.0
74	523.5
75	358.0
76	377.5
77	397.0
78	244.0
79	91.0
80	57.5
81	24.0
82	15.5
83	7.0
84	5.0
85	3.0
86	2.0
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	428137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.809665112465535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01310751375284	21.168769535804817
2	12.054131616761907	7.1865925343451025
3	4.430973983134297	3.9625755167784265
4	2.052026563767654	2.4468089867118863
5	1.2588544144517624	1.8763014260077961
6	0.8255082519825689	1.4764874723506314
7	0.5721283277267546	1.1938467695622637
8	0.4966272151785763	1.1843432776167773
9	0.4054611296374602	1.0877994441553196
>10	6.367859964142279	41.83079150481632
>50	0.413313597954984	8.330787771068254
>100	0.10136398124751507	5.037683534621968
>500	0.005500371075446555	1.2016234961924348
>1k	0.00314306918596946	2.015588729968012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	2978	0.6955717445584008	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	2312	0.5400140609197523	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1990	0.4648044901515169	No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTTG	1325	0.30948037660842204	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	972	0.22703013287802737	No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	847	0.1978338709338366	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	830	0.19386317930942665	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	671	0.15672553411641602	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	670	0.15649196402086246	No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	593	0.13850706666324097	No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	547	0.1277628422677788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5465540235952511	0.0
2	0.0	0.0	0.0	2.05144614924662	0.0
3	0.0	0.0	0.0	3.2664777863160626	0.0
4	0.0	0.0	0.0	3.9968514751119386	0.0
5	0.0	0.0	0.0	7.958667435890848	0.0
6	0.0	0.0	0.0	8.791811966730275	0.0
7	0.0	0.0	0.0	13.308824044639916	0.0
8	0.0	0.0	0.0	17.72540098146154	0.0
9	0.0	0.0	0.0	22.53694494986418	0.0
10	0.0	0.0	0.0	24.166096366350022	0.0
11	0.0	0.0	0.0	25.7212060625454	0.0
12	0.0	0.0	0.0	27.076613327042512	0.0
13	0.0	0.0	0.0	27.565475537036043	0.0
14	0.0	0.0	0.0	27.966515391101446	0.0
15	0.0	0.0	0.0	29.223589645370524	0.0
16	0.0	0.0	0.0	30.539757133814643	0.0
17	0.0	0.0	0.0	32.577889787614716	0.0
18	0.0	0.0	0.0	33.348671102941346	0.0
19	0.0	0.0	0.0	34.347883971719334	0.0
20	0.0	0.0	0.0	35.40992719620122	0.0
21	0.0	0.0	0.0	36.248210269142824	0.0
22	2.3357009555352608E-4	0.0	0.0	37.29834141875148	0.0
23	2.3357009555352608E-4	0.0	0.0	37.991811032449895	0.0
24	2.3357009555352608E-4	0.0	0.0	38.580874813435884	0.0
25	2.3357009555352608E-4	0.0	0.0	39.1809163889129	0.0
26	2.3357009555352608E-4	0.0	0.0	39.71439048715715	0.0
27	2.3357009555352608E-4	0.0	0.0	40.19904843543072	0.0
28	2.3357009555352608E-4	0.0	0.0	40.695852028673066	0.0
29	2.3357009555352608E-4	0.0	0.0	41.39119020313591	0.0
30	2.3357009555352608E-4	0.0	0.0	42.075083442916636	0.0
31	2.3357009555352608E-4	0.0	0.0	42.56861705482124	0.0
32	2.3357009555352608E-4	0.0	0.0	43.04766932080152	0.0
33	2.3357009555352608E-4	0.0	0.0	43.526721586781804	0.0
34	2.3357009555352608E-4	0.0	0.0	44.24354821003557	0.0
35	2.3357009555352608E-4	0.0	0.0	44.75016174729117	0.0
36	2.3357009555352608E-4	0.0	0.0	45.23762253671138	0.0
37	2.3357009555352608E-4	0.0	0.0	45.67416504530092	0.0
38	2.3357009555352608E-4	0.0	0.0	46.13383099335026	0.0
39	2.3357009555352608E-4	0.0	0.0	46.605876156463935	0.0
40	2.3357009555352608E-4	0.0	0.0	47.10338045999295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	75	0.0	46.000004	1
AGCGTGG	25	3.4135395E-5	46.0	1
CGGGTCC	20	6.3069945E-4	46.0	5
CGGGTAT	45	3.092282E-10	46.0	5
AGCCGTG	20	6.3069945E-4	46.0	14
CAAACGG	20	6.3069945E-4	46.0	1
ACCGGGT	20	6.3069945E-4	46.0	3
CCGGGAT	20	6.3069945E-4	46.0	4
CATGCGG	160	0.0	46.0	1
CCGGCTT	25	3.4135395E-5	46.0	4
ATAATAC	20	6.3069945E-4	46.0	10
TATGGGT	30	1.858647E-6	46.0	3
TATGGGA	115	0.0	46.0	3
CACGCTT	20	6.3069945E-4	46.0	22
ATAGCGG	85	0.0	46.0	1
GCAGTGC	30	1.858647E-6	46.0	38
CAACCGG	30	1.858647E-6	46.0	1
TGAACGA	30	1.858647E-6	46.0	27
CGACCCC	30	1.858647E-6	46.0	19
TACGGGT	20	6.3069945E-4	46.0	3
>>END_MODULE