FastQCFastQC Report
Thu 26 May 2016
SRR1544894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544894_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences728933
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT37250.5110209031557085Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT27900.3827512267931346Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT26110.3581947860777328Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.18163534920219007No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11580.15886233714209672No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT11360.15584422711003618Illumina Single End Adapter 2 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11110.15241455661905826No Hit
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC10640.1459667760960198No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC10360.14212554514612455No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA10230.14034211649081602No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC9270.12717218180546086No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT7910.10851477433454103Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG7620.10453635656500666No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC7600.10426198292572841No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC206.3099264E-446.00000412
CGGGTTC551.8189894E-1246.0000045
GACAGTA351.0188887E-746.0000048
ACTGCGA351.0188887E-746.00000425
GATGTCG206.3099264E-446.00000427
AGTCAAC206.3099264E-446.00000410
CGTGCTC206.3099264E-446.00000414
ACGTTCT206.3099264E-446.00000413
TCCGATC206.3099264E-446.00000414
TTTCGCA206.3099264E-446.00000413
CATTGCG206.3099264E-446.00000429
CGCATGG206.3099264E-446.0000042
ATTACGG405.6061253E-946.0000041
CCGGATA206.3099264E-446.00000419
CTCCCGC206.3099264E-446.00000427
ACCGAGT206.3099264E-446.00000442
ACCGAGG800.046.0000041
TCGGGCA405.6061253E-946.0000044
GAATCAC206.3099264E-446.0000048
AGGGTGC351.0188887E-746.0000045
Produced by FastQC (version 0.11.5)