##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544894_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	728933
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90527661664378	34.0	33.0	34.0	31.0	34.0
2	33.1868402171393	34.0	33.0	34.0	31.0	34.0
3	33.22366253139863	34.0	33.0	34.0	31.0	34.0
4	36.53321224310053	37.0	37.0	37.0	35.0	37.0
5	36.518544228344716	37.0	37.0	37.0	35.0	37.0
6	36.6521312658365	37.0	37.0	37.0	35.0	37.0
7	36.48413365837464	37.0	37.0	37.0	35.0	37.0
8	36.52289990986826	37.0	37.0	37.0	35.0	37.0
9	38.298047968743354	39.0	39.0	39.0	37.0	39.0
10	38.05769666073562	39.0	39.0	39.0	35.0	39.0
11	37.137354187559076	39.0	35.0	39.0	35.0	39.0
12	36.991575357405964	39.0	35.0	39.0	35.0	39.0
13	36.96246431427854	39.0	35.0	39.0	35.0	39.0
14	38.191763852096145	40.0	35.0	41.0	35.0	41.0
15	38.2925577522214	40.0	35.0	41.0	35.0	41.0
16	38.32699027208262	40.0	35.0	41.0	35.0	41.0
17	38.43313994564658	40.0	36.0	41.0	35.0	41.0
18	38.401466252728305	40.0	36.0	41.0	35.0	41.0
19	38.272691728869454	40.0	35.0	41.0	35.0	41.0
20	38.16833371516998	40.0	35.0	41.0	35.0	41.0
21	38.116686993180444	40.0	35.0	41.0	35.0	41.0
22	38.095099275242035	40.0	35.0	41.0	35.0	41.0
23	38.08975996422168	40.0	35.0	41.0	35.0	41.0
24	38.040680007627586	40.0	35.0	41.0	35.0	41.0
25	38.00827510896063	40.0	35.0	41.0	35.0	41.0
26	37.96867750534	40.0	35.0	41.0	35.0	41.0
27	37.94193156298316	40.0	35.0	41.0	35.0	41.0
28	37.99658267632279	40.0	35.0	41.0	35.0	41.0
29	37.99490899712319	40.0	35.0	41.0	35.0	41.0
30	37.91530908876399	40.0	35.0	41.0	35.0	41.0
31	37.82883612074087	40.0	35.0	41.0	35.0	41.0
32	37.75890513942982	40.0	35.0	41.0	35.0	41.0
33	37.66717105687354	40.0	35.0	41.0	35.0	41.0
34	37.55509216896478	40.0	35.0	41.0	35.0	41.0
35	37.48747004182826	39.0	35.0	41.0	35.0	41.0
36	37.410408089632384	39.0	35.0	41.0	34.0	41.0
37	37.30438874354707	39.0	35.0	41.0	34.0	41.0
38	37.28220426294323	39.0	35.0	41.0	35.0	41.0
39	37.151399374153726	39.0	35.0	41.0	34.0	41.0
40	37.079269288123875	38.0	35.0	41.0	34.0	41.0
41	37.02673908301586	38.0	35.0	41.0	34.0	41.0
42	36.96987514627545	38.0	35.0	41.0	34.0	41.0
43	36.90382929569659	38.0	35.0	41.0	34.0	41.0
44	36.85695804689868	38.0	35.0	41.0	34.0	41.0
45	36.84765129305437	38.0	35.0	41.0	34.0	41.0
46	36.90815342425161	38.0	35.0	41.0	34.0	41.0
47	36.8674843915696	38.0	35.0	41.0	34.0	41.0
48	36.91981156018454	38.0	35.0	41.0	34.0	41.0
49	36.82656567887584	38.0	35.0	41.0	34.0	41.0
50	36.75248342440252	37.0	35.0	41.0	34.0	41.0
51	36.69317207479974	37.0	35.0	41.0	34.0	41.0
52	36.516062518777446	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	4.0
15	9.0
16	5.0
17	34.0
18	63.0
19	131.0
20	211.0
21	364.0
22	584.0
23	919.0
24	1777.0
25	2746.0
26	3841.0
27	4301.0
28	4275.0
29	4609.0
30	5465.0
31	6722.0
32	8885.0
33	13055.0
34	38013.0
35	190667.0
36	23151.0
37	34234.0
38	62344.0
39	320308.0
40	2207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.915186992494508	22.195867109871553	25.247862286382972	21.641083611250966
2	25.523744980677236	25.1693914255494	26.69051888170792	22.616344712065445
3	24.200029357979403	25.177897008367022	27.69184547825383	22.930228155399742
4	25.832278138045613	28.077614815079027	23.71905236832466	22.371054678550703
5	22.34526355645855	35.18498956694236	21.106603761936967	21.363143114662115
6	86.47310520994384	2.68268825804292	7.964243627329261	2.8799629046839694
7	86.64842996544265	2.1328434849293423	5.454685135670905	5.764041413957113
8	77.20435211466624	3.8028186403963056	11.93415581404601	7.058673430891453
9	51.48593903692109	18.549853004322756	14.4540033171773	15.510204641578856
10	45.737399733583196	17.886280357728353	26.019675333672645	10.35664457501581
11	43.841340699350965	15.7050099254664	23.179908167142933	17.2737412080397
12	18.354910533615573	39.07986056331652	27.28810466805591	15.277124235011996
13	11.19046606478236	42.01305195402046	27.765789174039313	19.03069280715786
14	9.584419967267225	21.94179711989991	50.76941227794598	17.70437063488688
15	10.322759430564949	16.943669719987984	52.24238716041118	20.491183689035893
16	10.364738597374519	20.715621325965486	29.287053817017476	39.632586259642515
17	17.033115526392688	21.391129225868497	37.51373583031637	24.06201941742245
18	23.679954124727512	27.132672001404796	27.591836286736914	21.595537587130778
19	25.744067013017656	21.919435668298732	26.902335331230713	25.434161987452892
20	15.463012375623	33.60665520699433	28.316868628529647	22.61346378885302
21	18.309364509495385	25.196828789477223	23.958992115873475	32.53481458515392
22	13.83721137607983	33.73492488335691	22.236748782124007	30.191114958439254
23	14.13998268702336	22.394376437889356	41.663911498038914	21.80172937704837
24	15.732035728935307	22.66504603303733	37.838868592861076	23.764049645166292
25	11.993694893769387	32.616029182380274	30.94413341143836	24.446142512411978
26	15.23198977135073	31.335527407868764	27.55507021907363	25.87741260170688
27	15.80899753475285	26.59572278933729	39.3719313023282	18.22334837358166
28	12.86880961624731	23.139026494890476	33.810789194617335	30.181374694244877
29	15.474261694833407	29.459360462484206	29.872429976417585	25.1939478662648
30	25.354868005701487	25.201218767705676	21.669069722457344	27.7748435041355
31	26.430275484852515	23.203229926481583	25.561196982438716	24.805297606227185
32	22.329898632658967	25.655307140711148	23.82166810941472	28.19312611721516
33	15.245982826953918	25.324961279020158	27.184254245589102	32.24480164843683
34	14.836205796691877	20.852396585145687	31.193813423181556	33.11758419498088
35	28.69687611893	21.30017436444776	23.949526225318376	26.05342329130386
36	20.208441653759674	26.747588598677797	28.852720346040034	24.191249401522498
37	19.489719905670345	28.776581661140327	28.23688871268004	23.496809720509294
38	21.175059984936887	27.406222519765187	27.100981846068155	24.317735649229768
39	29.911116659555816	20.835385419510434	25.951630671131642	23.301867249802108
40	17.775159033820668	18.54326803698008	31.74900848226106	31.932564446938194
41	22.14620548116219	21.269032956389683	25.995942013875073	30.58881954857305
42	21.86236595132886	19.87370581384023	27.097277801937903	31.16665043289301
43	22.352671644719063	26.096637139490188	26.81782825033302	24.73286296545773
44	13.719367898009832	32.57638219150457	26.83333036095224	26.870919549533355
45	21.51349986898659	29.029005409276298	26.05877357726979	23.398721144467324
46	23.062887809990766	21.183291194115235	29.017070155967694	26.736750839926305
47	22.59082796361257	18.483180209978144	31.92021763317068	27.00577419323861
48	21.285358187926736	16.43827347643748	29.60930565634976	32.667062679286026
49	20.535769405418606	23.539886381875974	31.19271592862444	24.73162828408098
50	19.22481215694721	25.732543320167974	27.26505728235654	27.777587240528277
51	19.15608156030801	19.763956358128937	32.85569455628981	28.224267525273245
52	22.41852131814584	17.363598574903317	35.009801998263214	25.20807810868763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	239.0
1	231.5
2	224.0
3	1212.0
4	2200.0
5	1585.0
6	970.0
7	1147.5
8	1325.0
9	1442.0
10	1559.0
11	1493.5
12	1428.0
13	1422.0
14	1284.5
15	1153.0
16	1011.5
17	870.0
18	866.0
19	862.0
20	892.5
21	923.0
22	1014.0
23	1105.0
24	1357.0
25	1609.0
26	1963.0
27	2317.0
28	3611.0
29	4905.0
30	4576.0
31	4247.0
32	5028.0
33	5809.0
34	8315.0
35	10821.0
36	10843.0
37	10865.0
38	12706.0
39	16931.5
40	19316.0
41	28271.0
42	37226.0
43	52216.0
44	67206.0
45	73831.5
46	80457.0
47	80167.0
48	79877.0
49	86140.0
50	92403.0
51	92868.5
52	93334.0
53	80356.0
54	67378.0
55	53195.0
56	39012.0
57	31731.0
58	24450.0
59	21326.5
60	18203.0
61	15637.5
62	13072.0
63	11117.0
64	7940.5
65	6719.0
66	5203.0
67	3687.0
68	3038.0
69	2389.0
70	2245.0
71	2101.0
72	1747.0
73	1393.0
74	1237.0
75	1081.0
76	820.5
77	560.0
78	460.0
79	360.0
80	219.5
81	79.0
82	51.5
83	24.0
84	35.0
85	46.0
86	24.5
87	3.0
88	1.5
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	728933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.182224444161857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54526875162277	15.658495803413516
2	12.953883193643362	6.0059965523699335
3	5.660349806273849	3.9365849892452545
4	2.889011946371942	2.67894893450637
5	1.6801665198665647	1.9474998683556521
6	1.0790279561943816	1.5008560957214045
7	0.7819082173485605	1.2688460252516178
8	0.5203328346569679	0.9649978046947832
9	0.41586117110517934	0.8676528305555039
>10	4.5269720090260375	27.406733330936756
>50	1.6643848313185903	24.868833933885305
>100	0.27275276893713957	9.8786127001649
>500	0.004150585614260819	0.6821984336471315
>1k	0.005929408020372599	2.333742697251864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3725	0.5110209031557085	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT	2790	0.3827512267931346	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2611	0.3581947860777328	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.18163534920219007	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1158	0.15886233714209672	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	1136	0.15584422711003618	Illumina Single End Adapter 2 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1111	0.15241455661905826	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1064	0.1459667760960198	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1036	0.14212554514612455	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1023	0.14034211649081602	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	927	0.12717218180546086	No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	791	0.10851477433454103	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	762	0.10453635656500666	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	760	0.10426198292572841	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3718681963911634E-4	0.0	0.0	0.3920799305285945	0.0
2	1.3718681963911634E-4	0.0	0.0	1.5526804246755188	0.0
3	1.3718681963911634E-4	0.0	0.0	2.520944997688402	0.0
4	1.3718681963911634E-4	0.0	0.0	3.1048121020724815	0.0
5	1.3718681963911634E-4	0.0	0.0	6.32019678077409	0.0
6	1.3718681963911634E-4	0.0	0.0	6.940829952821453	0.0
7	1.3718681963911634E-4	0.0	0.0	10.253068526188278	0.0
8	1.3718681963911634E-4	0.0	0.0	13.585747935681331	0.0
9	1.3718681963911634E-4	0.0	0.0	17.0682353522203	0.0
10	1.3718681963911634E-4	0.0	0.0	18.340094357094546	0.0
11	1.3718681963911634E-4	0.0	0.0	19.585887866237364	0.0
12	1.3718681963911634E-4	0.0	0.0	20.720697238292132	0.0
13	1.3718681963911634E-4	0.0	0.0	21.08588855217146	0.0
14	1.3718681963911634E-4	0.0	0.0	21.39936043504684	0.0
15	1.3718681963911634E-4	0.0	0.0	22.509476179566573	0.0
16	1.3718681963911634E-4	0.0	0.0	23.69449592760926	0.0
17	1.3718681963911634E-4	0.0	0.0	25.72060806685937	0.0
18	1.3718681963911634E-4	0.0	0.0	26.35345086585461	0.0
19	1.3718681963911634E-4	0.0	0.0	27.355326209679077	0.0
20	1.3718681963911634E-4	0.0	0.0	28.365021202222977	0.0
21	5.487472785564654E-4	0.0	0.0	29.128877413973576	0.0
22	5.487472785564654E-4	0.0	0.0	30.159424803102617	0.0
23	5.487472785564654E-4	0.0	0.0	30.816275295534705	0.0
24	5.487472785564654E-4	0.0	0.0	31.348697342554118	0.0
25	5.487472785564654E-4	0.0	0.0	31.911026116254856	0.0
26	5.487472785564654E-4	0.0	0.0	32.39954838098975	0.0
27	5.487472785564654E-4	0.0	0.0	32.954606253249615	0.0
28	5.487472785564654E-4	0.0	0.0	33.39881717524107	0.0
29	5.487472785564654E-4	0.0	0.0	34.12727918752478	0.0
30	5.487472785564654E-4	0.0	0.0	34.786461855890735	0.0
31	5.487472785564654E-4	0.0	0.0	35.28033440659155	0.0
32	5.487472785564654E-4	0.0	0.0	35.781340671913604	0.0
33	5.487472785564654E-4	0.0	0.0	36.253126144652526	0.0
34	6.859340981955818E-4	0.0	0.0	37.06033339140909	0.0
35	8.231209178346981E-4	0.0	0.0	37.573412096859386	0.0
36	8.231209178346981E-4	0.0	0.0	38.03998447045202	0.0
37	8.231209178346981E-4	0.0	0.0	38.48584163427915	0.0
38	8.231209178346981E-4	0.0	0.0	38.89657897227866	0.0
39	8.231209178346981E-4	0.0	0.0	39.377281588294125	0.0
40	8.231209178346981E-4	0.0	0.0	39.8527711051633	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	6.3099264E-4	46.000004	12
CGGGTTC	55	1.8189894E-12	46.000004	5
GACAGTA	35	1.0188887E-7	46.000004	8
ACTGCGA	35	1.0188887E-7	46.000004	25
GATGTCG	20	6.3099264E-4	46.000004	27
AGTCAAC	20	6.3099264E-4	46.000004	10
CGTGCTC	20	6.3099264E-4	46.000004	14
ACGTTCT	20	6.3099264E-4	46.000004	13
TCCGATC	20	6.3099264E-4	46.000004	14
TTTCGCA	20	6.3099264E-4	46.000004	13
CATTGCG	20	6.3099264E-4	46.000004	29
CGCATGG	20	6.3099264E-4	46.000004	2
ATTACGG	40	5.6061253E-9	46.000004	1
CCGGATA	20	6.3099264E-4	46.000004	19
CTCCCGC	20	6.3099264E-4	46.000004	27
ACCGAGT	20	6.3099264E-4	46.000004	42
ACCGAGG	80	0.0	46.000004	1
TCGGGCA	40	5.6061253E-9	46.000004	4
GAATCAC	20	6.3099264E-4	46.000004	8
AGGGTGC	35	1.0188887E-7	46.000004	5
>>END_MODULE