FastQCFastQC Report
Thu 26 May 2016
SRR1544893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544893_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences418598
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT27840.6650772340049402Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT19200.4586739544861657Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT18100.43239575917706247Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTTG12090.2888212557155075Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.2116589185805952No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT7520.17964729884041492Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC6910.16507484507809403No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC6040.14429118151543963No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC5880.14046889856138825No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5450.13019651312237518No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC5430.12971872775311874No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT4490.10726281539806688Illumina Single End Adapter 2 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT4440.10606835197492581No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA4430.1058294592902976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA206.3068315E-446.0000042
GCGAGAC700.046.00000420
TCACGAC206.3068315E-446.00000424
AACCGAG206.3068315E-446.00000441
TGATAAC206.3068315E-446.00000437
TCGTTCA206.3068315E-446.00000415
CTCACGG405.5970304E-946.0000041
CTCACGA206.3068315E-446.00000423
GCGACTA206.3068315E-446.0000048
GGGCTTA206.3068315E-446.0000046
ACCGAGT206.3068315E-446.00000442
CACGACG206.3068315E-446.00000425
CGCGAAT206.3068315E-446.0000043
CGTTCAT206.3068315E-446.00000416
ATTCCGG206.3068315E-446.0000041
ATATCGG206.3068315E-446.0000041
TACGCGG405.5970304E-946.0000041
TAACCGA206.3068315E-446.00000440
TCGTGCG206.3068315E-446.00000435
GCTTAGG700.046.0000041
Produced by FastQC (version 0.11.5)