##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544893_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	418598
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92715445367632	34.0	33.0	34.0	31.0	34.0
2	33.254435042690126	34.0	33.0	34.0	31.0	34.0
3	33.367187611980945	34.0	34.0	34.0	31.0	34.0
4	36.610406643127774	37.0	37.0	37.0	35.0	37.0
5	36.60075298974195	37.0	37.0	37.0	35.0	37.0
6	36.41513098485898	37.0	37.0	37.0	35.0	37.0
7	36.251420217010114	37.0	37.0	37.0	35.0	37.0
8	36.39859483322902	37.0	37.0	37.0	35.0	37.0
9	38.267748532004454	39.0	39.0	39.0	37.0	39.0
10	38.10156522486968	39.0	39.0	39.0	35.0	39.0
11	37.27656844992093	39.0	35.0	39.0	35.0	39.0
12	37.21242098624456	39.0	35.0	39.0	35.0	39.0
13	37.1942125858222	39.0	35.0	39.0	35.0	39.0
14	38.38102427627461	40.0	35.0	41.0	35.0	41.0
15	38.444992570437506	40.0	35.0	41.0	35.0	41.0
16	38.49639272046211	40.0	35.0	41.0	35.0	41.0
17	38.621355094864285	41.0	36.0	41.0	35.0	41.0
18	38.60396848527704	40.0	36.0	41.0	35.0	41.0
19	38.50354277851304	40.0	35.0	41.0	35.0	41.0
20	38.41157865063856	40.0	35.0	41.0	35.0	41.0
21	38.36690094075939	40.0	35.0	41.0	35.0	41.0
22	38.3426413886354	40.0	35.0	41.0	35.0	41.0
23	38.322937520007265	40.0	35.0	41.0	35.0	41.0
24	38.29766028504675	40.0	35.0	41.0	35.0	41.0
25	38.26168543566859	40.0	35.0	41.0	35.0	41.0
26	38.220199332056055	40.0	35.0	41.0	35.0	41.0
27	38.2895642119647	40.0	35.0	41.0	35.0	41.0
28	38.326468353886064	40.0	35.0	41.0	35.0	41.0
29	38.31418210311564	40.0	36.0	41.0	35.0	41.0
30	38.2388353503839	40.0	35.0	41.0	35.0	41.0
31	38.14711489304774	40.0	35.0	41.0	35.0	41.0
32	38.09350259676348	40.0	35.0	41.0	35.0	41.0
33	38.00992360211946	40.0	35.0	41.0	35.0	41.0
34	37.88749587910119	40.0	35.0	41.0	35.0	41.0
35	37.84241682951185	40.0	35.0	41.0	35.0	41.0
36	37.80153990224511	40.0	35.0	41.0	35.0	41.0
37	37.7187564202409	40.0	35.0	41.0	35.0	41.0
38	37.72283909622119	40.0	35.0	41.0	35.0	41.0
39	37.64119035446897	40.0	35.0	41.0	35.0	41.0
40	37.612996239829144	40.0	35.0	41.0	35.0	41.0
41	37.55842359495268	39.0	35.0	41.0	35.0	41.0
42	37.50215958986904	39.0	35.0	41.0	35.0	41.0
43	37.45631608368888	39.0	35.0	41.0	35.0	41.0
44	37.33479854179905	39.0	35.0	41.0	35.0	41.0
45	37.37577102613964	39.0	35.0	41.0	35.0	41.0
46	37.34639200378406	39.0	35.0	41.0	35.0	41.0
47	37.291367851733646	39.0	35.0	41.0	35.0	41.0
48	37.280304731508515	39.0	35.0	41.0	35.0	41.0
49	37.20541187487757	39.0	35.0	41.0	35.0	41.0
50	37.13830930869235	39.0	35.0	41.0	35.0	41.0
51	37.09153650996899	38.0	35.0	41.0	34.0	41.0
52	36.66780777739024	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	4.0
16	10.0
17	10.0
18	18.0
19	70.0
20	89.0
21	136.0
22	265.0
23	425.0
24	763.0
25	1356.0
26	1929.0
27	2156.0
28	1826.0
29	1926.0
30	2185.0
31	2744.0
32	3750.0
33	5784.0
34	18950.0
35	105477.0
36	12009.0
37	18735.0
38	35633.0
39	201226.0
40	1119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.540040802870532	21.89690347302185	24.48387235486075	23.079183369246866
2	25.855833042680565	25.301602014343118	26.46453160311325	22.378033339863066
3	24.5469400236026	24.984830314526107	27.61073870395941	22.857490957911885
4	26.019474531650893	27.74690753419749	23.78773907185414	22.445878862297477
5	22.429156374373505	34.89816004854299	21.25165433184105	21.42102924524245
6	85.13848608927897	2.568574145122528	8.72531641336079	3.567623352237708
7	85.13753051854046	2.0279599998088855	6.290283278945433	6.544226202705221
8	75.08946531039327	3.878661627623639	13.35433996340164	7.677533098581455
9	50.36908919775058	18.03305319184516	15.229169752363845	16.368687858040413
10	44.11105643122996	17.44919947061381	27.69267889478688	10.747065203369342
11	42.08715760705976	15.669687862818266	23.755249666744703	18.487904863377274
12	17.91384574221568	37.1413623572019	28.836019283417503	16.108772617164917
13	11.476165676854643	40.19942761312763	28.58398750113474	19.740419208882987
14	9.80320020640328	21.7686181013765	49.87195352103928	18.55622817118094
15	10.325897400369806	16.933907949870758	51.77067257846431	20.96952207129513
16	10.494794528401952	21.350794795961757	29.94495912546166	38.20945155017463
17	16.574135566820672	21.58395405615889	37.658087234052715	24.183823142967718
18	23.07201658870802	27.713940343718797	27.922493657399222	21.291549410173964
19	24.971930109556183	21.958776678340556	26.935389084515453	26.133904127587805
20	15.262136942842536	34.32338424932752	27.75144649520542	22.663032312624523
21	17.827844375749528	25.30518540461254	24.26050769473337	32.606462524904565
22	13.66298931194129	34.30594508334966	22.24425343647127	29.786812168237788
23	13.945121572487206	22.666376810209318	40.88457183264134	22.503929784662134
24	16.148667695497828	22.81425138199418	36.947142604599165	24.08993831790883
25	12.169910033014968	32.099532248123495	31.116011065509152	24.61454665335238
26	15.641020740662878	29.621498430475064	27.678345333709192	27.05913549515287
27	16.418377536443078	25.89811704785976	38.72641531971008	18.95709009598708
28	13.471158486184837	22.814490274678807	33.275123149178924	30.43922808995743
29	15.897352591268952	28.28226604044931	29.97314846224779	25.847232906033952
30	25.130793744833944	24.512061691646878	21.248309834256254	29.108834729262924
31	26.55435525253346	22.698627322634128	25.211061686869023	25.53595573796339
32	21.914820424368962	24.480288964591328	24.08062150320833	29.52426910783138
33	15.274081577073947	24.417699081218736	27.354406853353336	32.95381248835398
34	15.13361267851256	20.04166288419916	31.376881877123157	33.44784256016513
35	28.14060267846478	21.15012494087406	23.784155681584718	26.92511669907644
36	20.543098629233775	26.11359824939441	28.401234597394158	24.94206852397766
37	19.80898140937128	28.389767748532	28.519247583600492	23.282003258496218
38	21.825474560318014	26.628411984768203	26.693868580356334	24.852244874557453
39	30.42178892397957	20.43034128208926	26.022341243866432	23.12552855006474
40	18.523977658756134	17.99291922082762	31.359920496514555	32.12318262390169
41	22.608803673213917	21.347211405692335	25.506333045069496	30.537651876024253
42	22.025905522721086	19.423408616381348	27.290622506557604	31.260063354339962
43	23.421277693634465	25.411492649272095	26.716085600026755	24.451144057066685
44	13.946316035910348	30.82050081462405	27.986039111510326	27.247144037955266
45	21.499624938485134	28.11528005389419	26.406002895379338	23.97909211224134
46	23.540007357894687	19.93965570786291	29.277492964610435	27.242843969631963
47	22.38806683261745	17.523017310163926	32.296140927572516	27.792774929646104
48	20.923654675846517	15.966870362495762	29.374483394569488	33.73499156708824
49	20.206498836592626	22.34267722253809	31.83245022670916	25.618373714160125
50	19.54619945628025	24.57178486280393	27.947577389285183	27.93443829163063
51	19.077730901724326	18.635540542477507	33.173593758211936	29.113134797586227
52	22.38973908140985	16.762621894992332	35.48798608689005	25.35965293670777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	129.5
2	159.0
3	799.5
4	1440.0
5	1011.0
6	582.0
7	661.5
8	741.0
9	798.5
10	856.0
11	846.0
12	836.0
13	824.0
14	746.0
15	680.0
16	610.0
17	540.0
18	528.0
19	516.0
20	524.5
21	533.0
22	575.5
23	618.0
24	732.5
25	847.0
26	1036.0
27	1225.0
28	1805.5
29	2386.0
30	2274.5
31	2163.0
32	2527.0
33	2891.0
34	4145.0
35	5399.0
36	5567.5
37	5736.0
38	6761.5
39	8999.5
40	10212.0
41	14895.5
42	19579.0
43	27588.0
44	35597.0
45	39236.5
46	42876.0
47	43977.5
48	45079.0
49	50423.0
50	55767.0
51	56804.5
52	57842.0
53	49535.0
54	41228.0
55	32798.0
56	24368.0
57	19503.0
58	14638.0
59	12687.0
60	10736.0
61	9104.5
62	7473.0
63	6488.0
64	4740.0
65	3977.0
66	3040.5
67	2104.0
68	1724.5
69	1345.0
70	1295.5
71	1246.0
72	1039.5
73	833.0
74	753.5
75	674.0
76	513.0
77	352.0
78	285.5
79	219.0
80	136.5
81	54.0
82	35.5
83	17.0
84	23.5
85	30.0
86	16.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	418598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.317970882027733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3021026913236	19.90812896958225
2	11.954093528590114	6.77031344927302
3	4.6971382830548185	3.990402753852122
4	2.2786243700966797	2.5810407425390625
5	1.2839613671541148	1.817959030435937
6	0.804645017588315	1.3671548507060758
7	0.5998463279261593	1.1890501593532967
8	0.4602420955309136	1.042649780794227
9	0.4010185351174132	1.022042808054749
>10	6.6870209619456515	44.58867745012307
>50	0.42301498540782967	8.12314932586526
>100	0.09898550658543213	4.644948361053373
>500	0.005922209795709616	1.1042174023156075
>1k	0.003384119883262637	1.8502649160519495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2784	0.6650772340049402	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT	1920	0.4586739544861657	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	1810	0.43239575917706247	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTTG	1209	0.2888212557155075	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.2116589185805952	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	752	0.17964729884041492	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	691	0.16507484507809403	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	604	0.14429118151543963	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	588	0.14046889856138825	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	545	0.13019651312237518	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	543	0.12971872775311874	No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	449	0.10726281539806688	Illumina Single End Adapter 2 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	444	0.10606835197492581	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	443	0.1058294592902976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.467990769186666	0.0
2	0.0	0.0	0.0	1.8731575401698048	0.0
3	0.0	0.0	0.0	2.9555802942202303	0.0
4	0.0	0.0	0.0	3.621135313594427	0.0
5	0.0	0.0	0.0	7.235342739334636	0.0
6	0.0	0.0	0.0	7.94007615898786	0.0
7	0.0	0.0	0.0	11.742531020215099	0.0
8	0.0	0.0	0.0	15.37369982656391	0.0
9	0.0	0.0	0.0	19.274817366542603	0.0
10	0.0	0.0	0.0	20.691689879072523	0.0
11	0.0	0.0	0.0	22.101395611063598	0.0
12	0.0	0.0	0.0	23.389743859263543	0.0
13	0.0	0.0	0.0	23.80661159393977	0.0
14	0.0	0.0	0.0	24.161367230612665	0.0
15	0.0	0.0	0.0	25.292285199642617	0.0
16	0.0	0.0	0.0	26.532138232863034	0.0
17	0.0	0.0	0.0	28.460479983181955	0.0
18	0.0	0.0	0.0	29.088528851069523	0.0
19	0.0	0.0	0.0	30.085905809392305	0.0
20	0.0	0.0	0.0	31.116966636247664	0.0
21	0.0	0.0	0.0	31.910568134582583	0.0
22	0.0	0.0	0.0	32.937806678483895	0.0
23	0.0	0.0	0.0	33.61912861504355	0.0
24	0.0	0.0	0.0	34.15854829693405	0.0
25	0.0	0.0	0.0	34.72997959856473	0.0
26	2.3889268462821132E-4	0.0	0.0	35.24718226078481	0.0
27	2.3889268462821132E-4	0.0	0.0	35.823869201477315	0.0
28	2.3889268462821132E-4	0.0	0.0	36.26367063387785	0.0
29	2.3889268462821132E-4	0.0	0.0	36.95478717050727	0.0
30	2.3889268462821132E-4	0.0	0.0	37.6105475898117	0.0
31	2.3889268462821132E-4	0.0	0.0	38.069699329667124	0.0
32	2.3889268462821132E-4	0.0	0.0	38.574479572286535	0.0
33	2.3889268462821132E-4	0.0	0.0	39.06755407335917	0.0
34	2.3889268462821132E-4	0.0	0.0	39.83965523007755	0.0
35	2.3889268462821132E-4	0.0	0.0	40.34204654585067	0.0
36	2.3889268462821132E-4	0.0	0.0	40.822220841953374	0.0
37	2.3889268462821132E-4	0.0	0.0	41.25485549381507	0.0
38	2.3889268462821132E-4	0.0	0.0	41.63660600385095	0.0
39	2.3889268462821132E-4	0.0	0.0	42.09504106565249	0.0
40	2.3889268462821132E-4	0.0	0.0	42.552281664030886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	6.3068315E-4	46.000004	2
GCGAGAC	70	0.0	46.000004	20
TCACGAC	20	6.3068315E-4	46.000004	24
AACCGAG	20	6.3068315E-4	46.000004	41
TGATAAC	20	6.3068315E-4	46.000004	37
TCGTTCA	20	6.3068315E-4	46.000004	15
CTCACGG	40	5.5970304E-9	46.000004	1
CTCACGA	20	6.3068315E-4	46.000004	23
GCGACTA	20	6.3068315E-4	46.000004	8
GGGCTTA	20	6.3068315E-4	46.000004	6
ACCGAGT	20	6.3068315E-4	46.000004	42
CACGACG	20	6.3068315E-4	46.000004	25
CGCGAAT	20	6.3068315E-4	46.000004	3
CGTTCAT	20	6.3068315E-4	46.000004	16
ATTCCGG	20	6.3068315E-4	46.000004	1
ATATCGG	20	6.3068315E-4	46.000004	1
TACGCGG	40	5.5970304E-9	46.000004	1
TAACCGA	20	6.3068315E-4	46.000004	40
TCGTGCG	20	6.3068315E-4	46.000004	35
GCTTAGG	70	0.0	46.000004	1
>>END_MODULE