FastQCFastQC Report
Thu 26 May 2016
SRR1544892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544892_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1136064
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT50810.44724592980677147TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT39490.3476036561320489TruSeq Adapter, Index 21 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT38600.33976959044560867TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.21160779674384542No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT16890.14867120162244382TruSeq Adapter, Index 15 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC16600.14611852853360374TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC13190.11610261393724297TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC12160.10703622331136274TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT11510.10131471466396258TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT206.3114235E-446.03
ACCGGAC206.3114235E-446.013
CTATCGA206.3114235E-446.020
ATATGCG253.4171302E-546.018
TATGCGT253.4171302E-546.019
TATGCGC206.3114235E-446.01
CCTCGCG206.3114235E-446.018
CGTTAGG253.4171302E-546.01
CTAACGG1500.046.01
CCGTAGT253.4171302E-546.032
GTGTCGT206.3114235E-446.046
GTCGTTA253.4171302E-546.023
TCGTCCG206.3114235E-446.040
GCGATCG253.4171302E-546.08
ATGCGCT405.6115823E-946.02
ATAACCG206.3114235E-446.010
ATTGCGG2250.046.01
ATGCCGG301.8613846E-646.01
ATACCGG405.6115823E-946.01
TACACGG750.046.01
Produced by FastQC (version 0.11.5)