##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544892_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1136064
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9306896442454	34.0	33.0	34.0	31.0	34.0
2	33.226961685257166	34.0	33.0	34.0	31.0	34.0
3	33.27218801053462	34.0	34.0	34.0	31.0	34.0
4	36.56033462903498	37.0	37.0	37.0	35.0	37.0
5	36.54896555123655	37.0	37.0	37.0	35.0	37.0
6	36.66294064418906	37.0	37.0	37.0	35.0	37.0
7	36.50908927666047	37.0	37.0	37.0	35.0	37.0
8	36.53994845360825	37.0	37.0	37.0	35.0	37.0
9	38.30976247817024	39.0	39.0	39.0	37.0	39.0
10	38.1157637245789	39.0	39.0	39.0	35.0	39.0
11	37.4038170384767	39.0	35.0	39.0	35.0	39.0
12	37.32112275364768	39.0	35.0	39.0	35.0	39.0
13	37.291399956340484	39.0	35.0	39.0	35.0	39.0
14	38.57205051828066	41.0	35.0	41.0	35.0	41.0
15	38.64368204608191	41.0	35.0	41.0	35.0	41.0
16	38.646248802884344	41.0	35.0	41.0	35.0	41.0
17	38.69566591318799	41.0	36.0	41.0	35.0	41.0
18	38.69024192299025	41.0	36.0	41.0	35.0	41.0
19	38.617091114585094	41.0	35.0	41.0	35.0	41.0
20	38.550665279420876	41.0	35.0	41.0	35.0	41.0
21	38.50743620077742	40.0	35.0	41.0	35.0	41.0
22	38.45410557855895	40.0	35.0	41.0	35.0	41.0
23	38.4683037223255	40.0	35.0	41.0	35.0	41.0
24	38.43148801475973	40.0	35.0	41.0	35.0	41.0
25	38.38089931553152	40.0	35.0	41.0	35.0	41.0
26	38.33198745845304	40.0	35.0	41.0	35.0	41.0
27	38.337185229001186	40.0	35.0	41.0	35.0	41.0
28	38.374634703678666	40.0	35.0	41.0	35.0	41.0
29	38.37117891245563	40.0	36.0	41.0	35.0	41.0
30	38.280980648977526	40.0	35.0	41.0	35.0	41.0
31	38.217547602951946	40.0	35.0	41.0	35.0	41.0
32	38.14625848543744	40.0	35.0	41.0	35.0	41.0
33	38.05895266463861	40.0	35.0	41.0	35.0	41.0
34	37.92443119401724	40.0	35.0	41.0	35.0	41.0
35	37.897258429102585	40.0	35.0	41.0	35.0	41.0
36	37.8393505999662	40.0	35.0	41.0	35.0	41.0
37	37.78369528477269	40.0	35.0	41.0	35.0	41.0
38	37.73631151061912	40.0	35.0	41.0	35.0	41.0
39	37.66369500309842	40.0	35.0	41.0	35.0	41.0
40	37.61681296124162	40.0	35.0	41.0	35.0	41.0
41	37.61338621767788	40.0	35.0	41.0	35.0	41.0
42	37.5363183764295	40.0	35.0	41.0	35.0	41.0
43	37.46276442172272	39.0	35.0	41.0	35.0	41.0
44	37.38174521857923	39.0	35.0	41.0	34.0	41.0
45	37.33101392175089	39.0	35.0	41.0	34.0	41.0
46	37.36172786040223	39.0	35.0	41.0	35.0	41.0
47	37.31685450819672	39.0	35.0	41.0	35.0	41.0
48	37.33640798405724	39.0	35.0	41.0	35.0	41.0
49	37.273496035434626	39.0	35.0	41.0	34.0	41.0
50	37.19785593065179	39.0	35.0	41.0	34.0	41.0
51	37.1287286631739	39.0	35.0	41.0	34.0	41.0
52	36.95183017858149	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	10.0
15	19.0
16	17.0
17	40.0
18	103.0
19	143.0
20	244.0
21	455.0
22	749.0
23	1236.0
24	2244.0
25	3551.0
26	5023.0
27	5659.0
28	5876.0
29	6278.0
30	7561.0
31	9283.0
32	12174.0
33	18071.0
34	59898.0
35	230972.0
36	38810.0
37	59508.0
38	102711.0
39	560435.0
40	4985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.872148047997296	22.432450988676695	25.207558729085683	20.487842234240325
2	26.879207509436085	23.381605261675396	27.4069066531463	22.33228057574221
3	26.89100261957073	24.851328798377555	26.126521041068106	22.131147540983605
4	26.52702664638612	27.135355050419697	23.200277449157795	23.137340854036392
5	23.998735986704975	34.59585023378964	21.295631232043267	20.109782547462114
6	86.52575911216269	2.617810264210467	7.963547828291364	2.892882795335474
7	87.21489282293955	2.4005689820291813	5.503915272379021	4.880622922652245
8	76.49745084783956	4.507668582051715	11.973533181229227	7.0213473888795
9	50.77997366345558	16.046631175708413	16.87598585995155	16.297409300884457
10	42.22737451411188	19.93267984902259	24.37309869866486	13.466846938200664
11	39.28555081403864	16.891830037744352	25.51255915159709	18.31005999661991
12	22.114335107881246	31.2262337333108	28.56986930313785	18.0895618556701
13	15.22114951270351	33.75804532139035	30.89350599966199	20.127299166244157
14	14.40667075094361	22.427169596079093	43.926662582389724	19.23949707058757
15	17.466269505943327	18.205488423187425	44.32197481832009	20.00626725254915
16	17.198943017294802	21.446679060334628	28.492848994422847	32.86152892794772
17	20.30167314517492	22.159138921750888	34.08778026590051	23.45140766717368
18	24.21298447974762	26.92929271590333	27.952562531688358	20.905160272660694
19	26.067897583234746	23.224923947946593	25.8716938482339	24.835484620584754
20	20.564246380485606	30.87563728804011	27.473364176666102	21.08675215480818
21	20.643731339079487	24.22574784519182	26.364975916849755	28.765544898878936
22	18.183482620697426	29.175821080502505	24.827562531688354	27.813133767111715
23	19.402604078643456	21.916546955101122	36.79766280772914	21.88318615852628
24	20.178528674440877	21.7794948171934	33.320922060728975	24.721054447636753
25	16.00464410455749	28.48712748577545	30.780748267703228	24.727480141963834
26	16.83135809250183	28.62682031998197	29.211030364486508	25.330791223029685
27	19.397058616415976	28.149822545208718	34.11973269111599	18.333386147259308
28	17.52630133513605	24.97693791899048	31.16584981127824	26.330910934595238
29	19.767020167877867	28.282825615458286	30.264756210917692	21.685398005746155
30	26.51646386119092	27.142660976846372	23.918106726381613	22.422768435581094
31	28.06479212438736	23.76661878204045	27.842357472818435	20.32623162075376
32	24.307961523294463	30.43983437552814	24.26588642893358	20.986317672243818
33	20.822066362458454	26.192010309278352	28.832002422398737	24.15392090586446
34	19.435172666328658	27.58805841924399	30.252520984733255	22.7242479296941
35	27.398368401780182	27.723966255422226	25.602694918596136	19.274970424201456
36	23.88263337276773	28.9553229395527	25.06742577882936	22.094617908850207
37	23.279058222071995	30.224529603965973	25.742739845642497	20.75367232831953
38	21.206903836403583	28.93014830150414	24.27961804968734	25.583329812404937
39	25.576023885978255	24.004017379302574	25.578312489437216	24.841646245281957
40	17.694073573319812	21.699921835389553	29.516646949467635	31.089357641822996
41	20.822858571348092	25.188457692524363	25.476733705143374	28.511950030984167
42	19.964896343867952	22.88885133231931	29.64137583798096	27.504876485831787
43	22.139509745929807	25.538349952115375	28.49769027097065	23.82445003098417
44	18.214730860233228	30.626091487803503	26.869877049180328	24.289300602782944
45	23.243496845248153	27.25559475522506	26.83475578840629	22.6661526111205
46	23.342258886823277	24.179183567122976	27.30532786885246	25.173229677201288
47	20.786240916004733	22.038018984845927	31.747507182693933	25.42823291645541
48	21.271512872514226	19.736564137231703	29.273262773928227	29.718660216325844
49	21.726328798377555	24.779237789420314	30.226466114585094	23.267967297617034
50	20.050895019998872	25.561412033124892	28.08750211255704	26.300190834319192
51	19.423201509774096	21.656438369669313	31.23802884344544	27.68233127711115
52	21.43514801982987	20.193668666553997	34.2504471579066	24.120736155709537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	239.0
1	380.5
2	522.0
3	2653.5
4	4785.0
5	3191.0
6	1597.0
7	1708.0
8	1819.0
9	1830.5
10	1842.0
11	1792.5
12	1743.0
13	1652.0
14	1498.5
15	1436.0
16	1360.0
17	1284.0
18	1306.0
19	1328.0
20	1356.5
21	1385.0
22	1661.5
23	1938.0
24	2271.5
25	2605.0
26	3353.0
27	4101.0
28	5607.5
29	7114.0
30	6977.5
31	6841.0
32	8601.5
33	10362.0
34	12205.0
35	14048.0
36	16643.0
37	19238.0
38	21084.5
39	28829.5
40	34728.0
41	49605.0
42	64482.0
43	90634.5
44	116787.0
45	137463.5
46	158140.0
47	154959.5
48	151779.0
49	134105.0
50	116431.0
51	100210.5
52	83990.0
53	72585.5
54	61181.0
55	54323.5
56	47466.0
57	44413.0
58	41360.0
59	39001.5
60	36643.0
61	37282.0
62	37921.0
63	33932.5
64	23928.0
65	17912.0
66	14340.0
67	10768.0
68	8629.0
69	6490.0
70	5412.5
71	4335.0
72	3621.5
73	2908.0
74	2361.5
75	1815.0
76	1589.0
77	1363.0
78	872.5
79	382.0
80	308.0
81	234.0
82	166.0
83	98.0
84	91.0
85	84.0
86	86.5
87	89.0
88	50.5
89	7.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1136064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.695321327375325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.41501911669751	15.974205402612975
2	13.250045749869182	6.27928183291148
3	5.6054574465891776	3.9846934615157807
4	2.8237529676046575	2.67638935666088
5	1.658133672568037	1.9645005087620289
6	1.0142473140665107	1.4419749607339998
7	0.7053042294508218	1.1698687245275856
8	0.5304356061685954	1.005507370531678
9	0.40719655974249785	0.8683787993850244
>10	4.870844084653662	29.803091819304466
>50	1.4756668009078675	22.681042930917382
>100	0.23235326083852234	8.84835958299153
>500	0.007819580893603843	1.2408085039198988
>1k	0.003351248954401648	1.6135892711147772
>5k	3.723609949335164E-4	0.44830747411057426
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	5081	0.44724592980677147	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT	3949	0.3476036561320489	TruSeq Adapter, Index 21 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	3860	0.33976959044560867	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2404	0.21160779674384542	No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	1689	0.14867120162244382	TruSeq Adapter, Index 15 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1660	0.14611852853360374	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1319	0.11610261393724297	TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1216	0.10703622331136274	TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	1151	0.10131471466396258	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35350121119936906	0.0
2	0.0	0.0	0.0	1.407667173680356	0.0
3	0.0	0.0	0.0	2.2439756915103373	0.0
4	0.0	0.0	0.0	2.73699369049631	0.0
5	0.0	0.0	0.0	5.674240183651626	0.0
6	0.0	0.0	0.0	6.205636302180159	0.0
7	0.0	0.0	0.0	8.723980339135823	0.0
8	0.0	0.0	0.0	11.428493465156892	0.0
9	0.0	0.0	0.0	13.907931243310236	0.0
10	0.0	0.0	0.0	15.100909807898146	0.0
11	0.0	0.0	0.0	16.259383274181737	0.0
12	0.0	0.0	0.0	17.221300912624642	0.0
13	0.0	0.0	0.0	17.60666652582953	0.0
14	0.0	0.0	0.0	17.884907892513098	0.0
15	0.0	0.0	0.0	18.889692834206524	0.0
16	0.0	0.0	0.0	19.97475494338347	0.0
17	0.0	0.0	0.0	21.410061404991268	0.0
18	0.0	0.0	0.0	22.091537096501604	0.0
19	0.0	0.0	0.0	23.017453242070868	0.0
20	0.0	0.0	0.0	23.988877387189454	0.0
21	0.0	0.0	0.0	24.835660667004674	0.0
22	0.0	0.0	0.0	25.802507605205342	0.0
23	0.0	0.0	0.0	26.471043884851557	0.0
24	8.802320996000226E-5	0.0	0.0	27.090463213340094	0.0
25	8.802320996000226E-5	0.0	0.0	27.68285941637091	0.0
26	8.802320996000226E-5	0.0	0.0	28.243831333446003	0.0
27	8.802320996000226E-5	0.0	0.0	28.823552194242577	0.0
28	1.7604641992000452E-4	0.0	0.0	29.34614599177511	0.0
29	1.7604641992000452E-4	0.0	0.0	30.07128119542561	0.0
30	1.7604641992000452E-4	0.0	0.0	30.76138316151203	0.0
31	1.7604641992000452E-4	0.0	0.0	31.34893808799504	0.0
32	1.7604641992000452E-4	0.0	0.0	31.97531125007042	0.0
33	1.7604641992000452E-4	0.0	0.0	32.533290378006875	0.0
34	1.7604641992000452E-4	0.0	0.0	33.25974592980677	0.0
35	1.7604641992000452E-4	0.0	0.0	33.805049715508986	0.0
36	1.7604641992000452E-4	0.0	0.0	34.39718184890992	0.0
37	1.7604641992000452E-4	0.0	0.0	35.00533420652358	0.0
38	1.7604641992000452E-4	0.0	0.0	35.55847205791223	0.0
39	1.7604641992000452E-4	0.0	0.0	36.196376260492364	0.0
40	1.7604641992000452E-4	0.0	0.0	36.82759069911554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	20	6.3114235E-4	46.0	3
ACCGGAC	20	6.3114235E-4	46.0	13
CTATCGA	20	6.3114235E-4	46.0	20
ATATGCG	25	3.4171302E-5	46.0	18
TATGCGT	25	3.4171302E-5	46.0	19
TATGCGC	20	6.3114235E-4	46.0	1
CCTCGCG	20	6.3114235E-4	46.0	18
CGTTAGG	25	3.4171302E-5	46.0	1
CTAACGG	150	0.0	46.0	1
CCGTAGT	25	3.4171302E-5	46.0	32
GTGTCGT	20	6.3114235E-4	46.0	46
GTCGTTA	25	3.4171302E-5	46.0	23
TCGTCCG	20	6.3114235E-4	46.0	40
GCGATCG	25	3.4171302E-5	46.0	8
ATGCGCT	40	5.6115823E-9	46.0	2
ATAACCG	20	6.3114235E-4	46.0	10
ATTGCGG	225	0.0	46.0	1
ATGCCGG	30	1.8613846E-6	46.0	1
ATACCGG	40	5.6115823E-9	46.0	1
TACACGG	75	0.0	46.0	1
>>END_MODULE