FastQCFastQC Report
Thu 26 May 2016
SRR1544891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544891_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences676136
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT39870.589674266715572TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT29110.4305346853295786TruSeq Adapter, Index 21 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT26250.388235502916573TruSeq Adapter, Index 15 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTTG17300.2558656838269224TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.22599003750724705No Hit
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC11740.17363370682821208TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT10360.1532236118177408TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC8170.12083367843155814TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC8140.12038998071393921TruSeq Adapter, Index 21 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA8050.11905888756108239No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC7250.1072269484245773TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT6990.10338156820521316TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG351.018725E-746.00000419
CGACGGT351.018725E-746.00000427
CGTTGAT351.018725E-746.00000424
ATGACGG351.018725E-746.0000041
TTCGGGT351.018725E-746.0000043
CGGTCTA351.018725E-746.00000430
ATAACGG351.018725E-746.0000041
TAATCGT351.018725E-746.00000420
CGGCGTT206.3096016E-446.039
CCTTACG206.3096016E-446.021
CTCACGT301.8602586E-646.044
AGTCACG206.3096016E-446.026
ATTACGG453.092282E-1046.01
TCGATCA501.6370905E-1146.016
TTATCGG206.3096016E-446.01
CCTCGAT501.6370905E-1146.014
CGTTACA206.3096016E-446.025
AGTGCGG1050.046.01
CTAACGG1000.046.01
GTCGTTA253.4156517E-546.023
Produced by FastQC (version 0.11.5)