FastQCFastQC Report
Thu 26 May 2016
SRR1544890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544890_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2296815
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT67770.29506076893437216Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT54660.23798172686959984Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.21216336535593855No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT47150.20528427409260216Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35610.15504078473886665No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT26210.114114545577245No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG23140.10074821002126859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA253.41823E-546.045
TATACGG1700.046.01
ACGGTAC253.41823E-546.012
AATACGG800.043.1251
CATGCGG4350.042.8275871
CGAATAT4000.042.5514
CGGTAAT601.8189894E-1242.1666685
GCGTAGG4500.041.9111141
CTTATGA15100.041.5827825
CGTATAT508.731149E-1041.39999829
TACGGGT1500.041.3999983
CCGTAGG1000.041.3999981
ATCAACG16200.041.3148114
ATAGCGG4750.041.1578941
CGTAGGG8550.041.1578942
GCTACGA4250.041.1294110
CGCAAGC16000.041.112519
CGTTTTT33300.041.09611
TCACGAC1400.041.0714324
TTAATCG2800.041.0714319
Produced by FastQC (version 0.11.5)