##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544890_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2296815
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90160722565814	34.0	33.0	34.0	31.0	34.0
2	33.238937398092574	34.0	33.0	34.0	31.0	34.0
3	33.31173646985064	34.0	34.0	34.0	31.0	34.0
4	36.58223278757758	37.0	37.0	37.0	35.0	37.0
5	36.583824992435176	37.0	37.0	37.0	35.0	37.0
6	36.658320761576356	37.0	37.0	37.0	35.0	37.0
7	36.46834638401439	37.0	37.0	37.0	35.0	37.0
8	36.48665173294323	37.0	37.0	37.0	35.0	37.0
9	38.28968593465299	39.0	39.0	39.0	37.0	39.0
10	38.13050463359043	39.0	39.0	39.0	35.0	39.0
11	37.717397787806156	39.0	37.0	39.0	35.0	39.0
12	37.66505878793024	39.0	37.0	39.0	35.0	39.0
13	37.66814610667381	39.0	37.0	39.0	35.0	39.0
14	39.05468137398963	41.0	38.0	41.0	35.0	41.0
15	39.10319159357632	41.0	38.0	41.0	35.0	41.0
16	39.09961403073386	41.0	38.0	41.0	35.0	41.0
17	39.12939440050679	41.0	38.0	41.0	35.0	41.0
18	39.10677786412924	41.0	38.0	41.0	35.0	41.0
19	39.05216397489567	41.0	37.0	41.0	35.0	41.0
20	38.975147323576344	41.0	37.0	41.0	35.0	41.0
21	38.92163757202909	41.0	37.0	41.0	35.0	41.0
22	38.88074834063693	41.0	37.0	41.0	35.0	41.0
23	38.85218051954554	41.0	37.0	41.0	35.0	41.0
24	38.78372659530698	41.0	36.0	41.0	35.0	41.0
25	38.730829431190585	41.0	36.0	41.0	35.0	41.0
26	38.66691396564373	41.0	36.0	41.0	35.0	41.0
27	38.603891040419015	41.0	36.0	41.0	35.0	41.0
28	38.62549748238321	41.0	36.0	41.0	35.0	41.0
29	38.62426447058209	41.0	36.0	41.0	35.0	41.0
30	38.55858090442635	40.0	36.0	41.0	35.0	41.0
31	38.47457413853532	40.0	35.0	41.0	35.0	41.0
32	38.3873829629291	40.0	35.0	41.0	35.0	41.0
33	38.294536564764684	40.0	35.0	41.0	35.0	41.0
34	38.12450763339668	40.0	35.0	41.0	35.0	41.0
35	38.08789824169556	40.0	35.0	41.0	35.0	41.0
36	37.98426865028311	40.0	35.0	41.0	35.0	41.0
37	37.90548999375222	40.0	35.0	41.0	35.0	41.0
38	37.78686137107255	40.0	35.0	41.0	35.0	41.0
39	37.716903625237556	40.0	35.0	41.0	35.0	41.0
40	37.62545046074673	40.0	35.0	41.0	34.0	41.0
41	37.576068599343	40.0	35.0	41.0	34.0	41.0
42	37.52510193463557	40.0	35.0	41.0	34.0	41.0
43	37.4521909687981	39.0	35.0	41.0	34.0	41.0
44	37.36418518687835	39.0	35.0	41.0	34.0	41.0
45	37.292552077550866	39.0	35.0	41.0	34.0	41.0
46	37.26758489473467	39.0	35.0	41.0	34.0	41.0
47	37.20888273544016	39.0	35.0	41.0	34.0	41.0
48	37.16098466789881	39.0	35.0	41.0	34.0	41.0
49	37.09975683718541	39.0	35.0	41.0	34.0	41.0
50	37.030381637180184	38.0	35.0	41.0	34.0	41.0
51	36.95049623064983	38.0	35.0	41.0	34.0	41.0
52	36.7382344681657	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	12.0
13	18.0
14	23.0
15	45.0
16	71.0
17	123.0
18	208.0
19	420.0
20	627.0
21	1005.0
22	1680.0
23	2558.0
24	4150.0
25	6567.0
26	9310.0
27	11271.0
28	12390.0
29	13716.0
30	15891.0
31	19403.0
32	24493.0
33	34893.0
34	107230.0
35	330664.0
36	102403.0
37	168737.0
38	282151.0
39	1138515.0
40	8234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.5596837359561	21.76435629338889	23.065767160176158	20.610192810478857
2	28.605090092149343	24.20996031460958	24.613693310083747	22.57125628315733
3	28.925751529835882	24.2725252142641	24.331650568286953	22.47007268761306
4	27.177678655007043	26.837860254308683	20.868202271406275	25.116258819278
5	26.060697095760872	35.79134584195941	19.070495446955892	19.07746161532383
6	87.60231015558502	3.314807679329854	6.55925705814356	2.523625106941569
7	86.58590265215092	3.4989322170048527	5.583514562557281	4.331650568286953
8	75.25725842090024	6.445403743880113	10.987258442669523	7.310079392550119
9	48.72647557596063	22.035079011587786	14.782818816491533	14.455626595960059
10	35.69717195333538	23.930399270293865	24.313059606455028	16.05936916991573
11	36.21314733663791	17.687885180129875	25.307959065053126	20.79100841817909
12	23.3314829448606	26.25931997135163	28.647670796298353	21.761526287489414
13	17.945110947115896	28.238800251652833	29.858129627331763	23.95795917389951
14	16.089106001136354	24.93034920095872	36.952170723371275	22.028374074533648
15	18.604328167484102	19.232676554271894	39.936694944956386	22.226300333287618
16	19.946360503566897	21.57361389576435	28.84603244057532	29.633993160093436
17	21.178588610750104	23.509120238242957	29.542649277368877	25.76964187363806
18	24.303176355083018	26.940437083526536	26.50152493779429	22.254861623596152
19	27.773677897436233	22.798962911684225	25.444495965064668	23.982863225814878
20	25.01376906716475	27.347914394498467	25.466439395423663	22.17187714291312
21	22.65772384802433	23.938453902469288	24.41541874291138	28.988403506595002
22	22.293828627904293	27.520109368843375	23.862653282915687	26.323408720336637
23	21.828227349612398	22.18406793755701	31.971882802924924	24.015821909905675
24	22.09720852571931	22.05806736720197	29.651190888251776	26.193533218826943
25	20.86001702357395	27.173542492538584	27.352398865385325	24.614041618502146
26	21.433071448941252	26.870340014324185	26.067968033994905	25.628620502739665
27	22.753290970321945	25.87648548098127	30.033981840069835	21.336241708626947
28	21.06726053252003	25.449677052788317	26.583420954669833	26.89964146002181
29	22.861658426995643	25.004930741047932	25.15230874058207	26.98110209137436
30	26.00679636801397	24.934006439351887	23.00050287027906	26.058694322355088
31	27.315782942901368	23.698251709432412	23.095982915472078	25.88998243219415
32	26.805162801531683	25.089613225270647	23.36927440825665	24.735949564941016
33	22.53655605697455	24.942975381125603	24.847277643171086	27.673190918728764
34	23.94245944928085	22.843459312134414	26.972742689332836	26.241338549251896
35	29.586492599534576	23.295433023556534	22.60142849990095	24.516645877007946
36	25.75544830558839	25.756275538082086	26.303598679040324	22.184677477289203
37	26.272903999669104	24.746094047626823	26.577194941690998	22.40380701101308
38	24.768777633374913	23.671475499768157	26.562783680879825	24.9969631859771
39	28.42340371340313	20.982926356715712	28.50834742893964	22.08532250094152
40	19.869471420205805	21.293835158687138	32.07794271632674	26.758750704780315
41	22.626201936159422	24.620093477271787	25.022041392101674	27.73166319446712
42	23.880460550806227	22.429930142392834	27.126999780130312	26.562609526670627
43	24.182356872451635	25.662972420504044	26.535354392931083	23.619316314113238
44	21.972427034828666	25.530266913094874	26.73680727442132	25.760498777655144
45	23.163162901670358	25.294200882526457	25.56640391150354	25.97623230429965
46	24.224110344106947	22.513132315837368	28.184899523905933	25.077857816149756
47	24.53471437621228	20.57984643952604	29.65811351806741	25.22732566619427
48	22.352170287985754	21.419356804966878	27.52524691801473	28.70322598903264
49	22.156072648428367	23.66368209890653	29.28803582352083	24.89220942914427
50	21.593032090089974	23.656411160672498	27.68076662682889	27.069790122408637
51	22.38290850590927	21.72760975524803	29.283072428558675	26.606409310284025
52	23.742225647255	19.3401297013473	31.359469526278783	25.558175125118915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	895.5
2	1490.0
3	5423.5
4	9357.0
5	6353.0
6	3349.0
7	3365.0
8	3381.0
9	3378.0
10	3375.0
11	3251.0
12	3127.0
13	2978.5
14	2953.5
15	3077.0
16	2883.5
17	2690.0
18	2952.5
19	3215.0
20	3346.0
21	3477.0
22	4350.5
23	5224.0
24	5599.0
25	5974.0
26	7936.0
27	9898.0
28	14336.5
29	18775.0
30	20713.0
31	22651.0
32	25504.0
33	28357.0
34	31088.5
35	33820.0
36	37985.5
37	42151.0
38	47757.0
39	58505.5
40	63648.0
41	80719.5
42	97791.0
43	111876.0
44	125961.0
45	138441.0
46	150921.0
47	154632.0
48	158343.0
49	175955.5
50	193568.0
51	205258.0
52	216948.0
53	201401.0
54	185854.0
55	168788.5
56	151723.0
57	137744.5
58	123766.0
59	120075.0
60	116384.0
61	118308.0
62	120232.0
63	112605.5
64	87855.5
65	70732.0
66	57699.5
67	44667.0
68	39363.0
69	34059.0
70	29598.0
71	25137.0
72	22164.0
73	19191.0
74	16887.5
75	14584.0
76	11842.5
77	9101.0
78	7198.5
79	5296.0
80	3721.0
81	2146.0
82	1642.5
83	1139.0
84	788.0
85	437.0
86	299.0
87	161.0
88	124.0
89	76.0
90	65.0
91	36.5
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2296815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.94861459671676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83558464843739	13.93123163288067
2	12.076045669728762	4.818007618355388
3	4.610982999871575	2.7594816832935276
4	2.2847434410914005	1.8230986543483516
5	1.3152171963009824	1.3118380479991334
6	0.8656414433418974	1.0361008519303883
7	0.6179012139391574	0.8628391223000977
8	0.4355534945912699	0.6950951039883522
9	0.34087702529368874	0.612002196221316
>10	4.915380228476343	25.710057775597363
>50	2.3737824903221587	32.1220341446382
>100	0.30963731422522395	9.726433776272037
>500	0.012288926179866825	1.666693520938517
>1k	0.005925017979578647	2.3891331284306596
>5k	4.388902207095294E-4	0.5359527428059323
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	6777	0.29506076893437216	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT	5466	0.23798172686959984	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4873	0.21216336535593855	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	4715	0.20528427409260216	Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3561	0.15504078473886665	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2621	0.114114545577245	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	2314	0.10074821002126859	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24486081813293625	0.0
2	0.0	0.0	0.0	0.8874463115226956	0.0
3	0.0	0.0	0.0	1.4052067754695088	0.0
4	0.0	0.0	0.0	1.739974704101114	0.0
5	0.0	0.0	0.0	3.6019444317457	0.0
6	0.0	0.0	0.0	4.021220690390824	0.0
7	0.0	0.0	0.0	5.766986021947784	0.0
8	0.0	0.0	0.0	7.661522586712469	0.0
9	0.0	0.0	0.0	9.413035007172976	0.0
10	0.0	0.0	0.0	10.287245598796595	0.0
11	4.3538552299597484E-5	0.0	0.0	11.101460065351366	0.0
12	4.3538552299597484E-5	0.0	0.0	11.808700308906028	0.0
13	8.707710459919497E-5	0.0	0.0	12.112207556986522	0.0
14	1.3061565689879246E-4	0.0	0.0	12.319581681589506	0.0
15	1.3061565689879246E-4	0.0	0.0	13.012410664333	0.0
16	1.3061565689879246E-4	0.0	0.0	13.727792617167687	0.0
17	1.7415420919838993E-4	0.0	0.0	14.640012364948854	0.0
18	1.7415420919838993E-4	0.0	0.0	15.11741259091394	0.0
19	1.7415420919838993E-4	0.0	0.0	15.688943166950756	0.0
20	1.7415420919838993E-4	0.0	0.0	16.338364213051552	0.0
21	2.1769276149798743E-4	0.0	0.0	16.956872887019635	0.0
22	2.1769276149798743E-4	0.0	0.0	17.619137806048812	0.0
23	2.1769276149798743E-4	0.0	0.0	18.110992831377363	0.0
24	3.047698660971824E-4	0.0	0.0	18.551080518021696	0.0
25	3.047698660971824E-4	0.0	0.0	18.960038139771815	0.0
26	3.047698660971824E-4	0.0	0.0	19.34992587561471	0.0
27	3.047698660971824E-4	0.0	0.0	19.731933133491378	0.0
28	3.4830841839677987E-4	0.0	0.0	20.143938453902468	0.0
29	4.3538552299597486E-4	0.0	0.0	20.632441010703953	0.0
30	4.7892407529557233E-4	0.0	0.0	21.111844010074822	0.0
31	5.224626275951699E-4	0.0	0.0	21.579709293086296	0.0
32	5.224626275951699E-4	0.0	0.0	21.995067082024455	0.0
33	5.660011798947673E-4	0.0	0.0	22.381297579474186	0.0
34	5.660011798947673E-4	0.0	0.0	22.872325372309046	0.0
35	5.660011798947673E-4	0.0	0.0	23.30453258098715	0.0
36	5.660011798947673E-4	0.0	0.0	23.783064809312027	0.0
37	5.660011798947673E-4	0.0	0.0	24.192327200928244	0.0
38	5.660011798947673E-4	0.0	0.0	24.60228620938125	0.0
39	5.660011798947673E-4	0.0	0.0	25.03401449398406	0.0
40	5.660011798947673E-4	0.0	0.0	25.504143781715115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	25	3.41823E-5	46.0	45
TATACGG	170	0.0	46.0	1
ACGGTAC	25	3.41823E-5	46.0	12
AATACGG	80	0.0	43.125	1
CATGCGG	435	0.0	42.827587	1
CGAATAT	400	0.0	42.55	14
CGGTAAT	60	1.8189894E-12	42.166668	5
GCGTAGG	450	0.0	41.911114	1
CTTATGA	1510	0.0	41.58278	25
CGTATAT	50	8.731149E-10	41.399998	29
TACGGGT	150	0.0	41.399998	3
CCGTAGG	100	0.0	41.399998	1
ATCAACG	1620	0.0	41.31481	14
ATAGCGG	475	0.0	41.157894	1
CGTAGGG	855	0.0	41.157894	2
GCTACGA	425	0.0	41.12941	10
CGCAAGC	1600	0.0	41.1125	19
CGTTTTT	3330	0.0	41.0961	1
TCACGAC	140	0.0	41.07143	24
TTAATCG	280	0.0	41.07143	19
>>END_MODULE