FastQCFastQC Report
Thu 26 May 2016
SRR1544889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544889_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1376363
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT50460.36661839936121504Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA46990.34140702707062015No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT39160.2845179651007765Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT35840.26039642158355025Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.21883761769242563No Hit
TGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTTG23700.17219294619224726Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21820.15853375889936014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA206.3118915E-446.041
ATAAGCG301.861672E-646.032
GCGATAA206.3118915E-446.08
CCCGCAC7950.042.528332
TCACGAC650.042.4615424
ATCAACG8800.042.34090814
CTTATGA8700.042.2988525
CATGCGG3550.042.1126751
CGTAGGG4600.042.02
CTAACGG1100.041.818181
CAACGCA8950.041.63128716
AACGCAA8950.041.63128717
TATACGG1050.041.619051
GCTTATG8900.041.6067424
TACGGGA2600.041.5769233
AATGCGG1500.041.41
TAATCAA9200.041.2512
ACGCAAG9050.041.1712718
CGCAAGC9100.040.94505319
CGCACTT8150.040.9202534
Produced by FastQC (version 0.11.5)