##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544889_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1376363
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88001493791972	34.0	33.0	34.0	31.0	34.0
2	33.26684239550177	34.0	33.0	34.0	31.0	34.0
3	33.40227178440571	34.0	34.0	34.0	31.0	34.0
4	36.615326770626645	37.0	37.0	37.0	35.0	37.0
5	36.627594609852196	37.0	37.0	37.0	35.0	37.0
6	36.41316208006173	37.0	37.0	37.0	35.0	37.0
7	36.19958252292455	37.0	37.0	37.0	35.0	37.0
8	36.33560259902366	37.0	37.0	37.0	35.0	37.0
9	38.22883643341183	39.0	39.0	39.0	37.0	39.0
10	38.14758679214713	39.0	39.0	39.0	35.0	39.0
11	37.77890353053664	39.0	37.0	39.0	35.0	39.0
12	37.770021426033686	39.0	37.0	39.0	35.0	39.0
13	37.77652552415315	39.0	37.0	39.0	35.0	39.0
14	39.086286829855204	41.0	38.0	41.0	35.0	41.0
15	39.11492535036179	41.0	38.0	41.0	35.0	41.0
16	39.133743060515286	41.0	38.0	41.0	35.0	41.0
17	39.190867525500174	41.0	38.0	41.0	35.0	41.0
18	39.19263522777058	41.0	38.0	41.0	35.0	41.0
19	39.170737661503544	41.0	38.0	41.0	35.0	41.0
20	39.110684463328354	41.0	38.0	41.0	35.0	41.0
21	39.05515332801013	41.0	38.0	41.0	35.0	41.0
22	39.02228045944275	41.0	38.0	41.0	35.0	41.0
23	38.9713513077582	41.0	37.0	41.0	35.0	41.0
24	38.921891245260156	41.0	37.0	41.0	35.0	41.0
25	38.857334148040884	41.0	37.0	41.0	35.0	41.0
26	38.76687981295632	41.0	37.0	41.0	35.0	41.0
27	38.83530144300595	41.0	37.0	41.0	35.0	41.0
28	38.83188010721009	41.0	37.0	41.0	35.0	41.0
29	38.83948202618059	41.0	37.0	41.0	35.0	41.0
30	38.78192017658132	41.0	36.0	41.0	35.0	41.0
31	38.704827868810774	41.0	36.0	41.0	35.0	41.0
32	38.6247312663883	41.0	36.0	41.0	35.0	41.0
33	38.55016954102951	41.0	36.0	41.0	35.0	41.0
34	38.36414666770322	40.0	35.0	41.0	35.0	41.0
35	38.33514414438633	40.0	36.0	41.0	35.0	41.0
36	38.222869257601374	40.0	35.0	41.0	35.0	41.0
37	38.148646105714846	40.0	35.0	41.0	35.0	41.0
38	38.0180482910395	40.0	35.0	41.0	35.0	41.0
39	37.97766141635601	40.0	35.0	41.0	35.0	41.0
40	37.919261851706274	40.0	35.0	41.0	35.0	41.0
41	37.881191226442446	40.0	35.0	41.0	35.0	41.0
42	37.846818753482914	40.0	35.0	41.0	35.0	41.0
43	37.80445057008943	40.0	35.0	41.0	35.0	41.0
44	37.659955985448605	40.0	35.0	41.0	35.0	41.0
45	37.639188934895806	40.0	35.0	41.0	35.0	41.0
46	37.53993459574255	39.0	35.0	41.0	35.0	41.0
47	37.45021989111884	39.0	35.0	41.0	35.0	41.0
48	37.347621230736365	39.0	35.0	41.0	34.0	41.0
49	37.28815363388873	39.0	35.0	41.0	34.0	41.0
50	37.22408114719736	39.0	35.0	41.0	34.0	41.0
51	37.13626637740189	39.0	35.0	41.0	34.0	41.0
52	36.65923088603806	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	6.0
13	6.0
14	7.0
15	19.0
16	33.0
17	71.0
18	118.0
19	209.0
20	353.0
21	545.0
22	820.0
23	1277.0
24	2080.0
25	3283.0
26	5001.0
27	5959.0
28	5971.0
29	6474.0
30	7523.0
31	9059.0
32	11884.0
33	17756.0
34	62013.0
35	188293.0
36	59943.0
37	101151.0
38	175945.0
39	707938.0
40	2624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.72921024468109	21.323226503473283	22.275300919888142	21.672262331957484
2	29.06319045193746	24.20487909076312	24.1574352114958	22.574495245803615
3	29.20435960571448	23.86107444039109	24.176543542655534	22.758022411238894
4	27.063354652805984	26.50013114272906	21.24490414229386	25.1916100621711
5	25.920778166806286	35.352592303047956	19.156501591513287	19.570127938632467
6	86.40467667323229	3.3040702198475254	7.1574867967244105	3.1337663101957838
7	85.36069336359667	3.4437862685933873	6.168285546763463	5.027234821046482
8	73.36894409396358	6.5259673501830555	11.912046458674057	8.193042097179305
9	48.303172927490785	21.274765450684157	15.309260711018824	15.112800910806234
10	34.96548512274742	23.354376716026223	25.361841316571283	16.31829684465508
11	35.253926471432315	17.577775630411455	25.603783304259125	21.564514593897105
12	23.04399348137083	25.325223069786094	29.35853404952037	22.272249399322707
13	17.89731342676314	27.371194953656847	30.235628246327455	24.49586337325255
14	16.095535843378528	24.576365391978715	36.64156912093684	22.68652964370591
15	18.545398270659703	19.023687791665424	39.65705268159635	22.77386125607852
16	19.751620757024128	22.066199105904474	28.994095307705887	29.188084829365508
17	20.85612589120748	23.730367642838406	29.654822165373524	25.758684300580587
18	23.8065103464711	27.209682329443613	26.578308193405377	22.405499130679914
19	27.181564747090704	22.83169483631862	25.515797794622497	24.470942621968188
20	24.50356482991769	27.715072259280436	25.416042134233486	22.36532077656839
21	22.25597462297374	23.82198591505293	24.47261369275402	29.44942576921931
22	21.96513565098742	27.630283580712355	23.91745491560003	26.487125852700196
23	21.57119887704043	22.185353718459446	31.91621687011348	24.32723053438664
24	22.196397316696252	22.219574341943225	28.969828453685547	26.61419988767498
25	20.991773245866096	26.84386313784953	27.40875771871229	24.75560589757208
26	21.330564683880635	26.09471483903592	26.073354195077897	26.501366282005545
27	23.541318678284725	25.3160685080898	29.585509055387277	21.557103758238195
28	21.719996832231033	25.046081593300606	26.075025265863726	27.158896308604636
29	23.278597288651323	24.330572675958305	24.810751233504533	27.580078801885836
30	26.155454629338337	24.990064394349456	22.34584916915087	26.508631807161336
31	27.36734422532428	23.034112367158954	22.596655097528775	27.001888309987994
32	26.681478650617606	24.85398110818149	23.241179834099	25.223360407101907
33	22.546595629205378	24.59874320945855	24.651781543095826	28.202879618240246
34	24.305579269422385	22.554006464864283	26.924510467078814	26.215903798634514
35	29.995066708419216	22.673960285186393	22.588735675108964	24.742237331285423
36	26.51248253549391	25.006920412710894	26.091590663218934	22.38900638857627
37	27.015329531526206	24.17218422756206	26.55404133938503	22.258444901526705
38	25.06635240848526	23.057434702909045	26.407059765483382	25.469153123122318
39	28.90581917706303	20.34717585404432	28.61163806350505	22.135366905387606
40	20.034031719829727	20.674487762312705	32.27048387670985	27.020996641147722
41	22.843537642322556	24.372930687616567	25.049133113866034	27.73439855619484
42	23.846543390079507	21.816918937809284	27.31851989627736	27.018017775833847
43	24.82550024957079	25.21791126323506	26.37153134747156	23.585057139722586
44	22.281912547779907	24.665077454130923	27.174953119198932	25.87805687889023
45	23.243068870639505	24.609060255179774	25.70433817241527	26.44353270176545
46	24.608769634173544	21.564805214903334	28.235429170938193	25.590995979984932
47	24.420810498393227	19.976779381602093	29.8740957145753	25.72831440542938
48	22.41647007366516	20.61556435329924	27.287932035371483	29.68003353766412
49	22.222989138766444	22.585902120298208	29.601347900226905	25.589760840708447
50	21.86995727144656	22.737243009293334	28.113077727314668	27.279721991945436
51	22.57115310423195	20.948107439679795	29.339425718360634	27.141313737727618
52	23.621966007514004	19.016785542767426	31.739591953576202	25.62165649614237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	586.0
2	1028.0
3	3441.0
4	5854.0
5	3978.5
6	2103.0
7	2063.0
8	2023.0
9	2004.5
10	1986.0
11	1969.0
12	1952.0
13	1829.5
14	1753.5
15	1800.0
16	1701.0
17	1602.0
18	1750.5
19	1899.0
20	1942.5
21	1986.0
22	2405.5
23	2825.0
24	2962.5
25	3100.0
26	4146.0
27	5192.0
28	7365.5
29	9539.0
30	10649.5
31	11760.0
32	13309.5
33	14859.0
34	16622.5
35	18386.0
36	20400.0
37	22414.0
38	25906.5
39	32001.5
40	34604.0
41	44025.0
42	53446.0
43	61970.5
44	70495.0
45	78033.5
46	85572.0
47	89616.5
48	93661.0
49	108663.5
50	123666.0
51	131881.5
52	140097.0
53	129475.5
54	118854.0
55	107178.0
56	95502.0
57	85595.5
58	75689.0
59	72671.0
60	69653.0
61	70682.0
62	71711.0
63	68593.0
64	53933.5
65	42392.0
66	34712.0
67	27032.0
68	23602.5
69	20173.0
70	17626.5
71	15080.0
72	13328.5
73	11577.0
74	10247.0
75	8917.0
76	7217.0
77	5517.0
78	4368.5
79	3220.0
80	2277.5
81	1335.0
82	1020.5
83	706.0
84	476.5
85	247.0
86	179.0
87	111.0
88	79.0
89	33.5
90	20.0
91	12.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1376363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.409649012850636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1994516114323	17.37953623742505
2	11.155807279762534	5.446186803080151
3	3.896446269768709	2.853326575274559
4	1.9203481621153848	1.8750009847883737
5	1.1098093988365234	1.3545028948381153
6	0.7288504913316459	1.0674590805749529
7	0.5759499479184931	0.9841115254362015
8	0.4655332928510299	0.9090803425832199
9	0.4085906327791214	0.8976198542469206
>10	7.843354448966011	48.819032785128805
>50	0.5518479790520814	8.784047927712194
>100	0.13146185369935232	5.6365720129295465
>500	0.0065730926835646086	1.0972379044824057
>1k	0.005676761863078525	2.5282782608635133
>5k	2.987769401620276E-4	0.36800681063597335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	5046	0.36661839936121504	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4699	0.34140702707062015	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT	3916	0.2845179651007765	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	3584	0.26039642158355025	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3012	0.21883761769242563	No Hit
TGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTTG	2370	0.17219294619224726	Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2182	0.15853375889936014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2917108350050096	0.0
2	0.0	0.0	0.0	1.077186759597577	0.0
3	0.0	0.0	0.0	1.6776097584721472	0.0
4	0.0	0.0	0.0	2.0793206443358327	0.0
5	0.0	0.0	0.0	4.26210236689013	0.0
6	0.0	0.0	0.0	4.743588718964401	0.0
7	0.0	0.0	0.0	6.794501159941091	0.0
8	0.0	0.0	0.0	8.986510099443242	0.0
9	0.0	0.0	0.0	11.07956258632352	0.0
10	0.0	0.0	0.0	12.111993710961425	0.0
11	0.0	0.0	0.0	13.024688981031893	0.0
12	7.265525155791023E-5	0.0	0.0	13.828691994771727	0.0
13	7.265525155791023E-5	0.0	0.0	14.178018444262161	0.0
14	7.265525155791023E-5	0.0	0.0	14.42243071050297	0.0
15	1.4531050311582047E-4	0.0	0.0	15.19461072406044	0.0
16	1.4531050311582047E-4	0.0	0.0	15.985608447771408	0.0
17	1.4531050311582047E-4	0.0	0.0	16.923151813874682	0.0
18	1.4531050311582047E-4	0.0	0.0	17.43922206569052	0.0
19	2.1796575467373068E-4	0.0	0.0	18.04523951893505	0.0
20	2.9062100623164093E-4	0.0	0.0	18.734737856219617	0.0
21	2.9062100623164093E-4	0.0	0.0	19.400114649986957	0.0
22	2.9062100623164093E-4	0.0	0.0	20.089685642523083	0.0
23	2.9062100623164093E-4	0.0	0.0	20.604884031320225	0.0
24	2.9062100623164093E-4	0.0	0.0	21.06951436503306	0.0
25	2.9062100623164093E-4	0.0	0.0	21.500142041016797	0.0
26	2.9062100623164093E-4	0.0	0.0	21.90781065750823	0.0
27	2.9062100623164093E-4	0.0	0.0	22.307341885825178	0.0
28	2.9062100623164093E-4	0.0	0.0	22.737896906557353	0.0
29	2.9062100623164093E-4	0.0	0.0	23.220473087404994	0.0
30	2.9062100623164093E-4	0.0	0.0	23.70624609932118	0.0
31	2.9062100623164093E-4	0.0	0.0	24.18192003127082	0.0
32	2.9062100623164093E-4	0.0	0.0	24.607970426406407	0.0
33	2.9062100623164093E-4	0.0	0.0	25.00343296063611	0.0
34	2.9062100623164093E-4	0.0	0.0	25.495962910947185	0.0
35	3.6327625778955115E-4	0.0	0.0	25.927535105201173	0.0
36	3.6327625778955115E-4	0.0	0.0	26.405461349949103	0.0
37	3.6327625778955115E-4	0.0	0.0	26.819305662822963	0.0
38	3.6327625778955115E-4	0.0	0.0	27.223196206233386	0.0
39	3.6327625778955115E-4	0.0	0.0	27.657311334291897	0.0
40	3.6327625778955115E-4	0.0	0.0	28.132331368977514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	6.3118915E-4	46.0	41
ATAAGCG	30	1.861672E-6	46.0	32
GCGATAA	20	6.3118915E-4	46.0	8
CCCGCAC	795	0.0	42.5283	32
TCACGAC	65	0.0	42.46154	24
ATCAACG	880	0.0	42.340908	14
CTTATGA	870	0.0	42.29885	25
CATGCGG	355	0.0	42.112675	1
CGTAGGG	460	0.0	42.0	2
CTAACGG	110	0.0	41.81818	1
CAACGCA	895	0.0	41.631287	16
AACGCAA	895	0.0	41.631287	17
TATACGG	105	0.0	41.61905	1
GCTTATG	890	0.0	41.60674	24
TACGGGA	260	0.0	41.576923	3
AATGCGG	150	0.0	41.4	1
TAATCAA	920	0.0	41.25	12
ACGCAAG	905	0.0	41.17127	18
CGCAAGC	910	0.0	40.945053	19
CGCACTT	815	0.0	40.92025	34
>>END_MODULE