FastQCFastQC Report
Thu 26 May 2016
SRR1544888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544888_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1129724
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT51980.46011238143121685TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT44040.38982972832302404TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT37020.32769065718706514TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.18066359571010265No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC15580.13790979035587453TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT15500.1372016527930716TruSeq Adapter, Index 15 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14960.13242172424415166No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC13100.11595752590898309TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC12390.10967280503910688TruSeq Adapter, Index 15 (95% over 21bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG12200.10799097832744989No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC11390.10082108550407001TruSeq Adapter, Index 14 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT11360.10055553391801891TruSeq Adapter, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT750.046.00000427
CGATCTC351.01954356E-746.00000438
AGCGTGA206.311409E-446.046
CAAGTAT206.311409E-446.025
CGCATTA206.311409E-446.041
TAGCGGA206.311409E-446.045
ACCGAAT206.311409E-446.024
TCGATGG650.046.01
CCGCTCG253.4171193E-546.015
CGCCTAA206.311409E-446.021
ACGATAA253.4171193E-546.09
TAACCGG301.8613737E-646.01
CGGATTA206.311409E-446.05
GTGTCGA206.311409E-446.017
GGTATCG206.311409E-446.022
TACTCGT253.4171193E-546.014
ACGTAGG850.046.01
CACAACG206.311409E-446.011
GCGTATT253.4171193E-546.021
CCGTTGG253.4171193E-546.01
Produced by FastQC (version 0.11.5)