##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544888_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1129724
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92383803477664	34.0	33.0	34.0	31.0	34.0
2	33.2243432909277	34.0	33.0	34.0	31.0	34.0
3	33.27789619411467	34.0	34.0	34.0	31.0	34.0
4	36.56250995818448	37.0	37.0	37.0	35.0	37.0
5	36.56109014237106	37.0	37.0	37.0	35.0	37.0
6	36.66046043104333	37.0	37.0	37.0	35.0	37.0
7	36.49674699307087	37.0	37.0	37.0	35.0	37.0
8	36.52307997351566	37.0	37.0	37.0	35.0	37.0
9	38.324673991169526	39.0	39.0	39.0	37.0	39.0
10	38.09661651872493	39.0	39.0	39.0	35.0	39.0
11	37.27373411558929	39.0	35.0	39.0	35.0	39.0
12	37.171792402392086	39.0	35.0	39.0	35.0	39.0
13	37.13924462966176	39.0	35.0	39.0	35.0	39.0
14	38.364219048192304	40.0	35.0	41.0	35.0	41.0
15	38.42200749917679	40.0	35.0	41.0	35.0	41.0
16	38.42219604080289	40.0	35.0	41.0	35.0	41.0
17	38.46994575666269	40.0	36.0	41.0	35.0	41.0
18	38.456233557930965	40.0	36.0	41.0	35.0	41.0
19	38.3889277381024	40.0	35.0	41.0	35.0	41.0
20	38.295987338500375	40.0	35.0	41.0	35.0	41.0
21	38.257098193895146	40.0	35.0	41.0	35.0	41.0
22	38.23301355021226	40.0	35.0	41.0	35.0	41.0
23	38.23268072555775	40.0	35.0	41.0	35.0	41.0
24	38.17263862677964	40.0	35.0	41.0	35.0	41.0
25	38.11375521808866	40.0	35.0	41.0	35.0	41.0
26	38.03156523186194	40.0	35.0	41.0	35.0	41.0
27	38.0060501503022	40.0	35.0	41.0	35.0	41.0
28	38.04682913702816	40.0	35.0	41.0	35.0	41.0
29	37.98233285298002	40.0	35.0	41.0	35.0	41.0
30	37.947161430579506	40.0	35.0	41.0	35.0	41.0
31	37.8293406177084	40.0	35.0	41.0	35.0	41.0
32	37.738502501495944	40.0	35.0	41.0	35.0	41.0
33	37.6355162853936	40.0	35.0	41.0	35.0	41.0
34	37.5867194111128	40.0	35.0	41.0	35.0	41.0
35	37.52509551005378	39.0	35.0	41.0	35.0	41.0
36	37.51773176457259	39.0	35.0	41.0	35.0	41.0
37	37.46856754393109	39.0	35.0	41.0	35.0	41.0
38	37.41292740527775	39.0	35.0	41.0	35.0	41.0
39	37.324980260665434	39.0	35.0	41.0	34.0	41.0
40	37.26858329999185	39.0	35.0	41.0	34.0	41.0
41	37.22261189458664	39.0	35.0	41.0	34.0	41.0
42	37.14520714794056	39.0	35.0	41.0	34.0	41.0
43	37.05398575227223	38.0	35.0	41.0	34.0	41.0
44	36.98139899656908	38.0	35.0	41.0	34.0	41.0
45	36.936080848065544	38.0	35.0	41.0	34.0	41.0
46	36.9624563167641	38.0	35.0	41.0	34.0	41.0
47	36.898803601587645	38.0	35.0	41.0	34.0	41.0
48	36.91997071851178	38.0	35.0	41.0	34.0	41.0
49	36.856895135449015	37.0	35.0	41.0	34.0	41.0
50	36.78589637823043	37.0	35.0	41.0	34.0	41.0
51	36.71882689931346	37.0	35.0	41.0	34.0	41.0
52	36.538986513520115	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	2.0
13	8.0
14	17.0
15	27.0
16	33.0
17	49.0
18	92.0
19	207.0
20	328.0
21	553.0
22	835.0
23	1400.0
24	2166.0
25	3752.0
26	5082.0
27	6070.0
28	6428.0
29	7101.0
30	8432.0
31	10376.0
32	13583.0
33	19551.0
34	63159.0
35	265141.0
36	41563.0
37	63037.0
38	109674.0
39	498014.0
40	3040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.92016457116959	21.743806451841337	24.127220453845364	21.208808523143706
2	27.749344087582458	24.468719793507088	25.807276821595366	21.974659297315096
3	27.90106256041299	24.218835751032998	25.438425668570375	22.441676019983642
4	26.324040208050814	26.380071592707598	22.74095265746324	24.55493554177835
5	24.096327952668084	34.72405649521476	21.11852098388633	20.061094568230825
6	86.38278021888533	2.8396316268398296	7.875374870322309	2.9022132839525407
7	86.78544494053415	2.4113854357347457	5.672358912442331	5.130810711288775
8	75.9366889611976	4.42391238922073	12.382227871586334	7.257170777995333
9	54.34061770839603	14.583384968363955	15.858829236167418	15.217168087072594
10	44.82342589871509	17.301039900010977	23.344728446948103	14.530805754325835
11	41.18280217114977	15.251512758868538	25.00655027245593	18.55913479752577
12	22.06893011036324	31.383063473910443	28.23663124798623	18.311375167740085
13	15.109885246307947	33.56704823479009	32.35896555264826	18.964100966253707
14	15.537334782654879	19.97284292446651	45.87846235009613	18.611359942782485
15	18.472210911691704	16.224228218573742	45.407285319246114	19.896275550488436
16	18.090082179364163	20.168642960581522	27.839277558058427	33.901997301995884
17	20.373383233426924	21.34645276191353	34.58216343106812	23.698000573591425
18	24.52501672974992	26.101685013330687	27.80165775003452	21.57164050688487
19	27.594173444133258	22.83531198770673	24.64947190641254	24.92104266174747
20	21.148793864696156	30.524181127425816	27.168759803279386	21.158265204598646
21	20.997340943451675	23.37500132775793	26.653058623168135	28.974599105622257
22	19.669937081977544	28.11846079219349	24.34727420148638	27.86432792434258
23	21.0270827210894	20.632207512631403	35.96674940073859	22.37396036554061
24	20.823404654588202	20.390467052129544	33.50004071790986	25.28608757537239
25	16.770290796690166	27.807234333341597	29.896948281173103	25.525526588795138
26	17.58287865000655	27.899203699310625	27.543984194369596	26.973933456313226
27	18.435033689644552	27.159908083744348	33.25405143203118	21.151006794579917
28	18.24773130428317	23.796520212016386	29.88597214894966	28.06977633475079
29	19.845643714748025	28.565118559931452	28.003653989824063	23.585583735496456
30	27.608159160998614	22.699615127234615	22.091236443591534	27.600989268175237
31	29.26360774844121	23.5123800149417	22.49611409512412	24.727898141492965
32	24.66186431376159	26.05087614319958	24.76932418891694	24.517935354121892
33	18.158417454174646	24.226271195442425	26.80769816344523	30.807613186937694
34	19.511402785105034	22.40281697122483	30.673244084395833	27.412536159274303
35	25.709907906709955	21.5432264871774	28.123063686351713	24.623801919760933
36	22.91214491327085	25.07214151421055	27.910445383120123	24.105268189398473
37	21.778416675223326	26.786542553756494	26.470359131964976	24.964681639055204
38	21.379115606997814	24.768616051354137	28.499084732200075	25.35318360944797
39	24.881918061402608	20.642298472901345	29.446218722449025	25.029564743247022
40	17.558093835308448	19.655951365112188	31.527257985136192	31.258696814443176
41	20.683193417153216	23.64666060028821	24.854566248039344	30.815579734519225
42	20.12048960631092	20.022943657034816	29.65821740531316	30.198349331341106
43	21.58208553593621	23.827501230389014	28.030209148429176	26.560204085245598
44	17.738049293455745	27.732260268879834	27.646575623780677	26.883114813883747
45	22.459556493444417	24.63044070941221	26.934100718405556	25.975902078737818
46	22.84903215298604	20.700011684269786	29.51508510043161	26.935871062312565
47	21.305557817661658	17.975186859799386	33.224663723174864	27.494591599364092
48	21.453115982310724	16.81269053326299	30.70581841228477	31.02837507214151
49	20.948745003204323	22.225782580524093	31.63259344760313	25.192878968668452
50	19.055185160269232	23.387039666325578	29.321320959809654	28.236454213595536
51	18.953567420007012	19.37340447755381	32.26858949619553	29.404438606243648
52	21.441343195329125	17.766640347553917	35.62905630047693	25.16296015664003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	367.0
2	520.0
3	2460.0
4	4400.0
5	2908.5
6	1417.0
7	1523.5
8	1630.0
9	1658.0
10	1686.0
11	1679.5
12	1673.0
13	1613.0
14	1490.0
15	1427.0
16	1337.5
17	1248.0
18	1298.5
19	1349.0
20	1391.0
21	1433.0
22	1551.0
23	1669.0
24	1998.0
25	2327.0
26	2933.5
27	3540.0
28	4083.0
29	4626.0
30	5074.0
31	5522.0
32	6494.5
33	7467.0
34	9645.5
35	11824.0
36	13053.0
37	14282.0
38	15830.5
39	21338.0
40	25297.0
41	39809.5
42	54322.0
43	69222.0
44	84122.0
45	96643.5
46	109165.0
47	118131.5
48	127098.0
49	128918.5
50	130739.0
51	120193.0
52	109647.0
53	99221.5
54	88796.0
55	78460.0
56	68124.0
57	60949.0
58	53774.0
59	51890.5
60	50007.0
61	47663.5
62	45320.0
63	40235.5
64	28589.0
65	22027.0
66	17684.0
67	13341.0
68	11250.5
69	9160.0
70	7386.5
71	5613.0
72	4743.5
73	3874.0
74	3366.0
75	2858.0
76	2613.0
77	2368.0
78	1592.0
79	816.0
80	714.5
81	613.0
82	403.0
83	193.0
84	120.0
85	47.0
86	31.0
87	15.0
88	28.5
89	24.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1129724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.512975715126448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.82152722607397	14.590428681773165
2	13.281752864151935	5.714600536416235
3	5.385218799581218	3.4755624376809937
4	2.68022533363432	2.306384900541672
5	1.4972440235684084	1.610508715932269
6	0.9336239352636815	1.2051017427833022
7	0.6244380867709777	0.9403464977412845
8	0.4630274174101974	0.7968878068946633
9	0.3552002541895741	0.6877272998148345
>10	4.611388749364812	26.2943539188975
>50	2.063521055231219	29.57363929576954
>100	0.2700512053463544	9.319848203659012
>500	0.007421254498067754	1.08874659008636
>1k	0.004947502998711836	1.9348202131278092
>5k	4.1229191655931967E-4	0.4610431588813767
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5198	0.46011238143121685	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	4404	0.38982972832302404	TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3702	0.32769065718706514	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.18066359571010265	No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1558	0.13790979035587453	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1550	0.1372016527930716	TruSeq Adapter, Index 15 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1496	0.13242172424415166	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1310	0.11595752590898309	TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1239	0.10967280503910688	TruSeq Adapter, Index 15 (95% over 21bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG	1220	0.10799097832744989	No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1139	0.10082108550407001	TruSeq Adapter, Index 14 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1136	0.10055553391801891	TruSeq Adapter, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39859293066271057	0.0
2	0.0	0.0	0.0	1.4530982788716535	0.0
3	0.0	0.0	0.0	2.290913532862894	0.0
4	0.0	0.0	0.0	2.798382613806558	0.0
5	0.0	0.0	0.0	5.492934557467134	0.0
6	0.0	0.0	0.0	6.036076068136996	0.0
7	0.0	0.0	0.0	8.52880880639873	0.0
8	0.0	0.0	0.0	11.159185783430289	0.0
9	8.851719535036877E-5	0.0	0.0	13.624301156742709	0.0
10	8.851719535036877E-5	0.0	0.0	14.730057961059515	0.0
11	8.851719535036877E-5	0.0	0.0	15.843161692590403	0.0
12	8.851719535036877E-5	0.0	0.0	16.772592243769274	0.0
13	8.851719535036877E-5	0.0	0.0	17.142151534357065	0.0
14	8.851719535036877E-5	0.0	0.0	17.40699498284537	0.0
15	8.851719535036877E-5	0.0	0.0	18.327839366075253	0.0
16	8.851719535036877E-5	0.0	0.0	19.247090439788835	0.0
17	8.851719535036877E-5	0.0	0.0	20.60051835669597	0.0
18	8.851719535036877E-5	0.0	0.0	21.232973717474355	0.0
19	8.851719535036877E-5	0.0	0.0	22.065832008525977	0.0
20	8.851719535036877E-5	0.0	0.0	22.922235873540792	0.0
21	8.851719535036877E-5	0.0	0.0	23.622760957543612	0.0
22	1.7703439070073754E-4	0.0	0.0	24.386752870612646	0.0
23	2.655515860511063E-4	0.0	0.0	24.95662657427832	0.0
24	2.655515860511063E-4	0.0	0.0	25.439753426500634	0.0
25	3.5406878140147507E-4	0.0	0.0	25.940141131816265	0.0
26	3.5406878140147507E-4	0.0	0.0	26.376973490870338	0.0
27	4.425859767518438E-4	0.0	0.0	26.827968601180466	0.0
28	5.311031721022126E-4	0.0	0.0	27.245681246038856	0.0
29	5.311031721022126E-4	0.0	0.0	27.838834972081678	0.0
30	5.311031721022126E-4	0.0	0.0	28.34382557155553	0.0
31	5.311031721022126E-4	0.0	0.0	28.802964263837893	0.0
32	5.311031721022126E-4	0.0	0.0	29.30406010671633	0.0
33	5.311031721022126E-4	0.0	0.0	29.757268146910217	0.0
34	5.311031721022126E-4	0.0	0.0	30.37697703155815	0.0
35	6.196203674525814E-4	0.0	0.0	30.825139591617067	0.0
36	6.196203674525814E-4	0.0	0.0	31.287199351345993	0.0
37	6.196203674525814E-4	0.0	0.0	31.806352702075905	0.0
38	6.196203674525814E-4	0.0	0.0	32.292489138940134	0.0
39	6.196203674525814E-4	0.0	0.0	32.917243503723036	0.0
40	6.196203674525814E-4	0.0	0.0	33.474901834430355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	75	0.0	46.000004	27
CGATCTC	35	1.01954356E-7	46.000004	38
AGCGTGA	20	6.311409E-4	46.0	46
CAAGTAT	20	6.311409E-4	46.0	25
CGCATTA	20	6.311409E-4	46.0	41
TAGCGGA	20	6.311409E-4	46.0	45
ACCGAAT	20	6.311409E-4	46.0	24
TCGATGG	65	0.0	46.0	1
CCGCTCG	25	3.4171193E-5	46.0	15
CGCCTAA	20	6.311409E-4	46.0	21
ACGATAA	25	3.4171193E-5	46.0	9
TAACCGG	30	1.8613737E-6	46.0	1
CGGATTA	20	6.311409E-4	46.0	5
GTGTCGA	20	6.311409E-4	46.0	17
GGTATCG	20	6.311409E-4	46.0	22
TACTCGT	25	3.4171193E-5	46.0	14
ACGTAGG	85	0.0	46.0	1
CACAACG	20	6.311409E-4	46.0	11
GCGTATT	25	3.4171193E-5	46.0	21
CCGTTGG	25	3.4171193E-5	46.0	1
>>END_MODULE