FastQCFastQC Report
Thu 26 May 2016
SRR1544887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544887_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences611236
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT33910.5547775327369461TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT28500.46626834806850387TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT22040.36058085583964294TruSeq Adapter, Index 15 (95% over 22bp)
TGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTTG16570.27109005359631955TruSeq Adapter, Index 15 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14700.24049630584585985No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.22380880707288184No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT8650.14151653371201958TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC8060.13186396089235583TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC7910.12940991695515316TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC7230.1182849177731678TruSeq Adapter, Index 15 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7200.11779410898572727No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6550.10715991859118244No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC6360.10405146293739242TruSeq Adapter, Index 14 (95% over 22bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG6160.10077940435445555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT405.6043064E-946.00000427
ACGTTGG206.309123E-446.0000041
TACGGGT405.6043064E-946.0000043
AAGCGAA206.309123E-446.00000433
TACGCGG800.046.0000041
ACAGCCG206.309123E-446.0000049
CGTACTA206.309123E-446.00000425
GCGATAA206.309123E-446.0000048
CTCGAGG800.046.0000041
CCCGCAT206.309123E-446.00000423
CGAAGAA206.309123E-446.00000436
GTCGAAT650.046.029
TCGGCGA301.8599621E-646.023
CTAACAC253.4152643E-546.027
CTTGCGT453.092282E-1046.015
TAACACC453.092282E-1046.046
TTGCGTC453.092282E-1046.016
CGGGACG301.8599621E-646.05
CTACCGG453.092282E-1046.01
GCGATAG453.092282E-1046.08
Produced by FastQC (version 0.11.5)