##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544887_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	611236
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92967691693552	34.0	33.0	34.0	31.0	34.0
2	33.27507542095034	34.0	33.0	34.0	31.0	34.0
3	33.400378904383906	34.0	34.0	34.0	31.0	34.0
4	36.61854341040122	37.0	37.0	37.0	35.0	37.0
5	36.62182201310132	37.0	37.0	37.0	35.0	37.0
6	36.42248493217023	37.0	37.0	37.0	35.0	37.0
7	36.26111845506482	37.0	37.0	37.0	35.0	37.0
8	36.39122859255672	37.0	37.0	37.0	35.0	37.0
9	38.282341027033745	39.0	39.0	39.0	37.0	39.0
10	38.12785896118684	39.0	39.0	39.0	35.0	39.0
11	37.36782519354227	39.0	35.0	39.0	35.0	39.0
12	37.3188621089072	39.0	35.0	39.0	35.0	39.0
13	37.29683624655616	39.0	35.0	39.0	35.0	39.0
14	38.44494270625421	41.0	35.0	41.0	35.0	41.0
15	38.474270167333074	41.0	35.0	41.0	35.0	41.0
16	38.493185938001034	41.0	35.0	41.0	35.0	41.0
17	38.56671563847679	41.0	36.0	41.0	35.0	41.0
18	38.567244075937936	40.0	36.0	41.0	35.0	41.0
19	38.53093731390167	40.0	35.0	41.0	35.0	41.0
20	38.45488485625847	40.0	35.0	41.0	35.0	41.0
21	38.41194563147459	40.0	35.0	41.0	35.0	41.0
22	38.394566092311315	40.0	35.0	41.0	35.0	41.0
23	38.37272510127021	40.0	35.0	41.0	35.0	41.0
24	38.33104234698218	40.0	35.0	41.0	35.0	41.0
25	38.262793749059284	40.0	35.0	41.0	35.0	41.0
26	38.174410538646285	40.0	35.0	41.0	35.0	41.0
27	38.24396796000236	40.0	35.0	41.0	35.0	41.0
28	38.28088496096434	40.0	35.0	41.0	35.0	41.0
29	38.20544765033473	40.0	35.0	41.0	35.0	41.0
30	38.176067836318545	40.0	35.0	41.0	35.0	41.0
31	38.06537573048708	40.0	35.0	41.0	35.0	41.0
32	37.95724073843818	40.0	35.0	41.0	35.0	41.0
33	37.87454763790091	40.0	35.0	41.0	35.0	41.0
34	37.812286907184784	40.0	35.0	41.0	35.0	41.0
35	37.732301435124896	40.0	35.0	41.0	35.0	41.0
36	37.73600376941149	40.0	35.0	41.0	35.0	41.0
37	37.69238068438377	40.0	35.0	41.0	35.0	41.0
38	37.641554162385724	39.0	35.0	41.0	35.0	41.0
39	37.58170330281593	39.0	35.0	41.0	35.0	41.0
40	37.571096924919345	39.0	35.0	41.0	35.0	41.0
41	37.53763194576236	39.0	35.0	41.0	35.0	41.0
42	37.48089281390494	39.0	35.0	41.0	35.0	41.0
43	37.42705927006917	39.0	35.0	41.0	35.0	41.0
44	37.235274100347496	39.0	35.0	41.0	35.0	41.0
45	37.24391233500645	39.0	35.0	41.0	35.0	41.0
46	37.21566465325995	39.0	35.0	41.0	35.0	41.0
47	37.148184989104045	38.0	35.0	41.0	35.0	41.0
48	37.12060807936705	38.0	35.0	41.0	35.0	41.0
49	37.07268714539065	38.0	35.0	41.0	35.0	41.0
50	37.00654084510729	38.0	35.0	41.0	35.0	41.0
51	36.93662676936568	37.0	35.0	41.0	34.0	41.0
52	36.51993665294584	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	4.0
15	11.0
16	14.0
17	22.0
18	45.0
19	78.0
20	155.0
21	231.0
22	364.0
23	552.0
24	1019.0
25	1736.0
26	2545.0
27	2898.0
28	2974.0
29	2948.0
30	3459.0
31	4352.0
32	5806.0
33	8956.0
34	32892.0
35	139960.0
36	21458.0
37	34014.0
38	61813.0
39	281945.0
40	978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.60148289694978	21.063877127656095	23.306382477471878	23.02825749792224
2	27.544679959950003	24.824454057025434	25.699402522102755	21.931463460921805
3	28.081133964622502	23.88913611109293	25.609911719859436	22.41981820442513
4	26.419909822065453	25.830775674207672	23.059832863247586	24.68948164047929
5	24.025253748143108	33.95955081179773	21.51296716816418	20.502228271894978
6	85.31401946220444	2.8241137629328117	8.427350483283053	3.4345162915796847
7	85.67819958248532	2.358336223651748	6.372497693198699	5.590966500664228
8	74.5176985648751	4.572701869654274	13.190486162464252	7.719113403006367
9	53.92794272588657	14.388877618464882	16.18016608969367	15.503013565954884
10	43.88779456707393	17.0390814677146	24.438841953026326	14.634282012185146
11	40.37409445778717	15.284767258472995	25.166547781871486	19.174590501868348
12	21.82692118919697	30.52012643234365	28.773992369559387	18.878960008900002
13	15.300800345529385	32.560254958804784	32.6682328920417	19.470711803624134
14	15.619008042719997	19.73247649025908	45.21772277810862	19.4307926889123
15	18.569750472812466	16.174603590102677	44.921437873423685	20.334208063661173
16	18.016936175225283	20.527259520054447	28.13152366679973	33.32428063792054
17	20.293634537232755	21.456687760537665	34.51040187423516	23.739275827994426
18	24.226485350993723	26.243545864445156	27.781249795496336	21.74871898906478
19	27.20536748489945	22.943511180624178	24.797459573716207	25.053661760760164
20	21.067312789168177	30.888233022924044	26.8321892035155	21.21226498439228
21	20.82665288039317	23.486836508320845	26.681020096983815	29.005490514302167
22	19.89264375789384	28.247027334777403	24.321375049898894	27.538953857429867
23	21.200649176422854	20.869844053687935	35.352956959341405	22.576549810547807
24	20.94133853372511	21.06976683310538	32.61064466098201	25.3782499721875
25	17.001452794010824	27.665746127518666	29.796510676727156	25.53629040174335
26	17.92221007924926	26.996937353166373	27.241850938099194	27.83900162948517
27	18.87307030345071	26.681183699912964	33.06071631906497	21.385029677571346
28	18.830042733085094	23.461478054303083	29.693277228435498	28.015201984176326
29	20.39981283824906	27.79041155952856	27.87172221531454	23.93805338690784
30	27.30222041895438	22.363211590940324	21.96467485553861	28.36989313456668
31	29.236825056115805	23.449698643404513	22.017027792865605	25.296448507614077
32	24.115562565032164	25.716090020875733	25.288595567015033	24.87975184707707
33	17.99321375049899	23.907132433299086	26.99955500003272	31.100098816169204
34	20.108272418509383	22.015391763574137	30.775019795954428	27.101316021962056
35	25.22119116020653	21.27607012675955	28.401632102821168	25.10110661021275
36	23.168465208201088	24.67246693584802	27.773396854897292	24.385671001053602
37	22.066272274538804	26.299661669142527	26.736481489964596	24.897584566354077
38	21.548305400859896	24.322356667473773	28.37071114921241	25.758626782453913
39	25.059715069138598	20.405048131981754	29.497444522246724	25.03779227663292
40	17.63099686536788	19.312671374068284	31.4528267314098	31.603505029154043
41	20.964242943805665	23.683160023297056	24.686863993612942	30.665733039284337
42	20.035469115039035	20.15555366503282	29.70701987448383	30.101957345444312
43	22.166233664247525	23.67268943583166	27.74542075401318	26.415656145907633
44	18.000248676452305	26.885523758417374	28.052176246163512	27.062051318966812
45	22.32869137289034	24.093639772526487	27.263282921817435	26.314385932765738
46	22.85598361353062	19.994568382752327	29.978764339796736	27.170683663920318
47	21.430347688945023	17.357125561976062	33.41442585188045	27.798100897198463
48	21.23287895346478	16.592281868214567	30.511291874169714	31.663547304150935
49	20.732581196133737	21.562538855695674	31.909442506658642	25.795437441511954
50	19.28731292005052	23.00747992592059	29.45425334895196	28.25095380507693
51	19.333776151928227	18.925914049565144	32.088096905286996	29.65221289321964
52	21.609002087573376	17.698564875105525	35.296186742927446	25.396246294393656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	228.5
2	348.0
3	1571.0
4	2794.0
5	1852.5
6	911.0
7	948.5
8	986.0
9	1013.0
10	1040.0
11	1012.5
12	985.0
13	964.0
14	883.0
15	823.0
16	780.0
17	737.0
18	741.5
19	746.0
20	742.5
21	739.0
22	835.5
23	932.0
24	1082.5
25	1233.0
26	1535.0
27	1837.0
28	2034.5
29	2232.0
30	2463.0
31	2694.0
32	3076.5
33	3459.0
34	4604.5
35	5750.0
36	6430.5
37	7111.0
38	8051.0
39	10740.0
40	12489.0
41	19687.5
42	26886.0
43	34668.5
44	42451.0
45	49595.5
46	56740.0
47	63151.5
48	69563.0
49	71143.5
50	72724.0
51	66621.5
52	60519.0
53	55188.0
54	49857.0
55	43773.0
56	37689.0
57	33526.5
58	29364.0
59	28370.0
60	27376.0
61	26172.5
62	24969.0
63	22805.0
64	16655.0
65	12669.0
66	10133.0
67	7597.0
68	6332.5
69	5068.0
70	4102.0
71	3136.0
72	2639.0
73	2142.0
74	1910.5
75	1679.0
76	1510.5
77	1342.0
78	893.0
79	444.0
80	394.0
81	344.0
82	225.5
83	107.0
84	62.5
85	18.0
86	10.0
87	2.0
88	11.0
89	10.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	611236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.69492234866471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0757037302011	18.97360391954417
2	11.678012993030764	6.234873000713077
3	4.070697394042113	3.2600085251659796
4	1.8569241487927404	1.9828178383753008
5	1.0373167850467802	1.3845545513895159
6	0.7327190889677596	1.1735927510027218
7	0.5543974326796951	1.0359717490978473
8	0.43811317986667314	0.9356317853173932
9	0.37813200840399996	0.9084784141700469
>10	7.692103724779201	49.688864822101145
>50	0.376879927355864	6.701926391587884
>100	0.09914651683708188	4.446306002582356
>500	0.006158168747644837	1.1461398842756754
>1k	0.0036949012485869024	2.127230364676884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3391	0.5547775327369461	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	2850	0.46626834806850387	TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	2204	0.36058085583964294	TruSeq Adapter, Index 15 (95% over 22bp)
TGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTTG	1657	0.27109005359631955	TruSeq Adapter, Index 15 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1470	0.24049630584585985	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1368	0.22380880707288184	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	865	0.14151653371201958	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	806	0.13186396089235583	TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	791	0.12940991695515316	TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	723	0.1182849177731678	TruSeq Adapter, Index 15 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	720	0.11779410898572727	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	655	0.10715991859118244	No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	636	0.10405146293739242	TruSeq Adapter, Index 14 (95% over 22bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG	616	0.10077940435445555	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4767389355339018	0.0
2	1.6360292914684345E-4	0.0	0.0	1.655334437107762	0.0
3	1.6360292914684345E-4	0.0	0.0	2.540753489650479	0.0
4	1.6360292914684345E-4	0.0	0.0	3.095694625316572	0.0
5	1.6360292914684345E-4	0.0	0.0	5.915881917949859	0.0
6	1.6360292914684345E-4	0.0	0.0	6.482275258656231	0.0
7	1.6360292914684345E-4	0.0	0.0	9.129697858110452	0.0
8	1.6360292914684345E-4	0.0	0.0	11.797734426637174	0.0
9	1.6360292914684345E-4	0.0	0.0	14.415708498844964	0.0
10	1.6360292914684345E-4	0.0	0.0	15.608701058183746	0.0
11	1.6360292914684345E-4	0.0	0.0	16.763083326243873	0.0
12	1.6360292914684345E-4	0.0	0.0	17.781020751395534	0.0
13	1.6360292914684345E-4	0.0	0.0	18.16303359095341	0.0
14	1.6360292914684345E-4	0.0	0.0	18.443285408581954	0.0
15	1.6360292914684345E-4	0.0	0.0	19.40183497045331	0.0
16	1.6360292914684345E-4	0.0	0.0	20.315884535596727	0.0
17	1.6360292914684345E-4	0.0	0.0	21.58757010385514	0.0
18	1.6360292914684345E-4	0.0	0.0	22.21416932248755	0.0
19	1.6360292914684345E-4	0.0	0.0	23.01795051338599	0.0
20	1.6360292914684345E-4	0.0	0.0	23.852161849105748	0.0
21	1.6360292914684345E-4	0.0	0.0	24.53504047536467	0.0
22	1.6360292914684345E-4	0.0	0.0	25.28139703813257	0.0
23	1.6360292914684345E-4	0.0	0.0	25.84435471732686	0.0
24	1.6360292914684345E-4	0.0	0.0	26.32632894659346	0.0
25	3.272058582936869E-4	0.0	0.0	26.82335464534157	0.0
26	3.272058582936869E-4	0.0	0.0	27.245613805469574	0.0
27	3.272058582936869E-4	0.0	0.0	27.68685090537861	0.0
28	3.272058582936869E-4	0.0	0.0	28.091768155017046	0.0
29	3.272058582936869E-4	0.0	0.0	28.66274237773953	0.0
30	3.272058582936869E-4	0.0	0.0	29.163367340928872	0.0
31	3.272058582936869E-4	0.0	0.0	29.610494146287195	0.0
32	3.272058582936869E-4	0.0	0.0	30.09443161070356	0.0
33	3.272058582936869E-4	0.0	0.0	30.536650328187477	0.0
34	3.272058582936869E-4	0.0	0.0	31.09420911071992	0.0
35	3.272058582936869E-4	0.0	0.0	31.534300990124926	0.0
36	3.272058582936869E-4	0.0	0.0	31.97570169296311	0.0
37	3.272058582936869E-4	0.0	0.0	32.48058033231027	0.0
38	3.272058582936869E-4	0.0	0.0	32.95764647370247	0.0
39	3.272058582936869E-4	0.0	0.0	33.54334496004817	0.0
40	3.272058582936869E-4	0.0	0.0	34.07603609735029	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	40	5.6043064E-9	46.000004	27
ACGTTGG	20	6.309123E-4	46.000004	1
TACGGGT	40	5.6043064E-9	46.000004	3
AAGCGAA	20	6.309123E-4	46.000004	33
TACGCGG	80	0.0	46.000004	1
ACAGCCG	20	6.309123E-4	46.000004	9
CGTACTA	20	6.309123E-4	46.000004	25
GCGATAA	20	6.309123E-4	46.000004	8
CTCGAGG	80	0.0	46.000004	1
CCCGCAT	20	6.309123E-4	46.000004	23
CGAAGAA	20	6.309123E-4	46.000004	36
GTCGAAT	65	0.0	46.0	29
TCGGCGA	30	1.8599621E-6	46.0	23
CTAACAC	25	3.4152643E-5	46.0	27
CTTGCGT	45	3.092282E-10	46.0	15
TAACACC	45	3.092282E-10	46.0	46
TTGCGTC	45	3.092282E-10	46.0	16
CGGGACG	30	1.8599621E-6	46.0	5
CTACCGG	45	3.092282E-10	46.0	1
GCGATAG	45	3.092282E-10	46.0	8
>>END_MODULE