FastQCFastQC Report
Thu 26 May 2016
SRR1544886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544886_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1669307
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT34920.20918860341447082Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT33690.20182027631825664Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.19948397748287164No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT30480.1825907397500879No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT25370.1519792344967103Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19950.11951067119469337No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA17210.10309667424865529No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16890.10117971110167273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT2600.046.014
ACGCGCC206.31228E-446.031
ATTACGG1550.044.516131
TACGGGA3500.044.0285723
ACGGGTA1000.043.74
TACGCGG1700.043.2941171
TATACGG1550.043.0322571
CATGCGG3600.042.8055571
AACACGT2450.042.244940
TCTACGG1100.041.8181841
CTAACGG1600.041.68751
CTTAATC11100.041.64864710
AGTACGG2050.041.5121961
AACGGGA4350.041.241383
CGGTCTA950.041.15789430
CACGAGG1850.041.0270271
CGTTTTT24000.041.016671
AACGCAA10100.040.99009717
CAACGCA10250.040.83902416
GCTACGA2950.040.5423710
Produced by FastQC (version 0.11.5)