##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544886_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1669307
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.915724309548814	34.0	33.0	34.0	31.0	34.0
2	33.256856288268125	34.0	33.0	34.0	31.0	34.0
3	33.3266007990142	34.0	34.0	34.0	31.0	34.0
4	36.599933984581625	37.0	37.0	37.0	35.0	37.0
5	36.59862326102988	37.0	37.0	37.0	35.0	37.0
6	36.671513388489956	37.0	37.0	37.0	35.0	37.0
7	36.49133023464228	37.0	37.0	37.0	35.0	37.0
8	36.51480105217315	37.0	37.0	37.0	35.0	37.0
9	38.31894133314004	39.0	39.0	39.0	37.0	39.0
10	38.13292282366275	39.0	39.0	39.0	35.0	39.0
11	37.702507088270764	39.0	37.0	39.0	35.0	39.0
12	37.671215061100206	39.0	37.0	39.0	35.0	39.0
13	37.66555402930677	39.0	37.0	39.0	35.0	39.0
14	39.05099181876072	41.0	38.0	41.0	35.0	41.0
15	39.091285785059306	41.0	38.0	41.0	35.0	41.0
16	39.09628965792392	41.0	38.0	41.0	35.0	41.0
17	39.115227456663156	41.0	38.0	41.0	35.0	41.0
18	39.095834978227494	41.0	38.0	41.0	35.0	41.0
19	39.03665533062522	41.0	37.0	41.0	35.0	41.0
20	38.961918328983224	41.0	37.0	41.0	35.0	41.0
21	38.915590721179505	41.0	37.0	41.0	35.0	41.0
22	38.87337499932607	41.0	37.0	41.0	35.0	41.0
23	38.854841560000644	41.0	36.0	41.0	35.0	41.0
24	38.77856020492336	41.0	36.0	41.0	35.0	41.0
25	38.73639899670941	41.0	36.0	41.0	35.0	41.0
26	38.68887328693883	41.0	36.0	41.0	35.0	41.0
27	38.643272927029	41.0	36.0	41.0	35.0	41.0
28	38.661063543134965	41.0	36.0	41.0	35.0	41.0
29	38.667215796734816	41.0	36.0	41.0	35.0	41.0
30	38.603313231179165	41.0	36.0	41.0	35.0	41.0
31	38.550563197782076	41.0	35.0	41.0	35.0	41.0
32	38.47377624367477	40.0	36.0	41.0	35.0	41.0
33	38.386992925806936	40.0	35.0	41.0	35.0	41.0
34	38.27333737892431	40.0	35.0	41.0	35.0	41.0
35	38.18545540155286	40.0	35.0	41.0	35.0	41.0
36	38.118409615487145	40.0	35.0	41.0	35.0	41.0
37	38.02356307138231	40.0	35.0	41.0	35.0	41.0
38	37.95094611117069	40.0	35.0	41.0	35.0	41.0
39	37.828047806664685	40.0	35.0	41.0	35.0	41.0
40	37.71649912209078	40.0	35.0	41.0	35.0	41.0
41	37.63724946939059	40.0	35.0	41.0	35.0	41.0
42	37.59297660646005	40.0	35.0	41.0	35.0	41.0
43	37.52663230909593	39.0	35.0	41.0	34.0	41.0
44	37.46168020621731	39.0	35.0	41.0	34.0	41.0
45	37.421233481917945	39.0	35.0	41.0	34.0	41.0
46	37.439126535742076	39.0	35.0	41.0	34.0	41.0
47	37.36793471782003	39.0	35.0	41.0	34.0	41.0
48	37.3305311725165	39.0	35.0	41.0	34.0	41.0
49	37.26144501880122	39.0	35.0	41.0	34.0	41.0
50	37.19082888887424	39.0	35.0	41.0	34.0	41.0
51	37.10974014965492	39.0	35.0	41.0	34.0	41.0
52	36.903813378845236	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	8.0
14	16.0
15	35.0
16	25.0
17	62.0
18	177.0
19	255.0
20	411.0
21	739.0
22	1136.0
23	1959.0
24	3127.0
25	4821.0
26	6646.0
27	7883.0
28	8432.0
29	9348.0
30	10827.0
31	13201.0
32	17118.0
33	23892.0
34	74420.0
35	251260.0
36	69060.0
37	110960.0
38	188185.0
39	857942.0
40	7356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.36354127790754	21.990802171200386	23.55564315012158	20.0900134007705
2	28.111126353630578	24.259408245457546	25.429294911001993	22.200170489909883
3	28.35254389995369	25.004268238256955	24.409290801512245	22.23389706027711
4	26.681011940883252	27.33481618420099	21.129127236631728	24.855044638284028
5	25.419111044283643	37.22604649713923	18.748738248866147	18.606104209710974
6	88.71435871292698	3.2648278597046563	5.677325980182196	2.3434874471861677
7	87.55501534469094	3.3922460038806523	4.93923526349557	4.113503387932837
8	77.14255077106847	6.29632536136253	9.889972305873036	6.671151561695961
9	49.40145820990387	22.805571413766312	13.952376644919118	13.8405937314107
10	35.30297303012567	23.367900571913975	25.35585126043322	15.973275137527128
11	34.97714920023699	18.547277403137947	26.1547456519382	20.320827744686866
12	22.883627756907508	27.913798959688062	28.702209958982976	20.500363324421453
13	17.645226432285973	29.767801848311905	29.13035169684186	23.45662002256026
14	15.66578226773146	24.914889831528892	38.38652806224379	21.032799838495855
15	18.519361627310015	20.67450744530515	39.145286037858824	21.66084488952601
16	20.241932730168866	22.202626598941958	27.936203466468424	29.619237204420756
17	20.214975435914425	24.151878593931496	31.210915667399707	24.42223030275438
18	24.43259388476775	26.38340341231421	26.260717770907327	22.92328493201071
19	27.011508368442712	23.763993082159242	25.30684888998848	23.917649659409562
20	23.55366628187625	28.4446180361072	26.229986455457265	21.771729226559287
21	22.25450441410717	24.304396974313295	24.614825193927782	28.826273417651755
22	21.050172317015384	28.20535707332444	24.645616414476187	26.09885419518399
23	20.979604111167088	22.72164437098748	33.138841447379065	23.159910070466367
24	21.03477670674118	23.38245751081137	30.431071097167866	25.15169468527958
25	20.201317073492174	27.740613320377854	27.614213562873697	24.443856043256275
26	20.79377849610647	27.111909313265926	26.704494739433787	25.38981745119382
27	21.444827104900416	25.519092653418458	31.879696185303242	21.156384056377885
28	20.304593463035857	24.85803989320119	28.92128290362408	25.916083740138873
29	21.900944523685578	26.127488832192043	26.90769283301394	25.063873811108444
30	24.93447879868712	24.880144874489833	24.697673944936433	25.487702381886617
31	26.8000433712912	25.00995922259956	24.62662649830139	23.56337090780785
32	26.250414093992298	24.888771208651256	24.787411782254555	24.073402915101898
33	21.190230436941796	25.15205411586964	27.6239181888053	26.033797258383267
34	23.176923118395838	24.332612275632943	28.96836831092184	23.522096295049383
35	28.18199408497059	24.778785448093128	25.228612831552255	21.810607635384024
36	24.494775376847997	29.329536148833018	24.768601581374785	21.407086892944196
37	24.225801485287008	29.01383628056433	25.743197626320384	21.017164607828278
38	24.94628010306073	27.2430415735392	24.467997797888586	23.342680525511486
39	28.965492866201366	23.446016820153513	26.05967626086753	21.52881405277759
40	21.376774913182537	22.374254705695236	28.943088359421004	27.305882021701223
41	22.711101073679078	25.275338808259956	25.01511106105707	26.998449057003892
42	23.625672210084783	22.549776643840826	27.692629336604952	26.13192180946944
43	23.571278380789153	26.489076005791624	27.56005935397144	22.379586259447784
44	20.662346710341478	27.97352434273624	26.790578365752975	24.57355058116931
45	22.734823492622986	26.979818571419155	26.246699977895016	24.038657958062835
46	24.44954702759888	23.341063087856217	27.583661962718658	24.625727921826243
47	23.805087979622684	21.909870383338713	29.519615025876007	24.765426611162596
48	22.789516847410333	22.024289121174238	27.992334543616003	27.193859487799426
49	22.16770192660787	24.955325772910555	29.08152904169215	23.795443258789426
50	21.73668474402851	25.03877357490264	27.63859493789938	25.58594674316947
51	21.501617138129774	22.90477425662266	29.66170991914609	25.93189868610148
52	23.53665323394678	21.15919959600002	30.423043813989874	24.881103356063324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	731.5
2	1180.0
3	3762.5
4	6345.0
5	4243.5
6	2142.0
7	2202.5
8	2263.0
9	2309.0
10	2355.0
11	2313.5
12	2272.0
13	2314.5
14	2298.5
15	2240.0
16	2081.0
17	1922.0
18	2195.0
19	2468.0
20	2585.5
21	2703.0
22	3216.5
23	3730.0
24	4437.5
25	5145.0
26	6914.0
27	8683.0
28	13162.0
29	17641.0
30	17491.5
31	17342.0
32	19767.5
33	22193.0
34	25567.5
35	28942.0
36	30222.5
37	31503.0
38	36665.0
39	48532.5
40	55238.0
41	65110.0
42	74982.0
43	92682.0
44	110382.0
45	123138.5
46	135895.0
47	149210.0
48	162525.0
49	166177.5
50	169830.0
51	153727.5
52	137625.0
53	122906.0
54	108187.0
55	96261.5
56	84336.0
57	78457.5
58	72579.0
59	72759.0
60	72939.0
61	72696.0
62	72453.0
63	67901.0
64	54385.5
65	45422.0
66	37346.0
67	29270.0
68	24911.5
69	20553.0
70	17990.5
71	15428.0
72	13610.5
73	11793.0
74	10691.5
75	9590.0
76	7415.0
77	5240.0
78	4276.5
79	3313.0
80	2453.5
81	1594.0
82	1291.5
83	989.0
84	564.5
85	140.0
86	99.5
87	59.0
88	53.0
89	28.0
90	9.0
91	5.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1669307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.336875435769954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35270393839754	15.224437559389603
2	11.202775398603977	4.7806444642984225
3	3.9968400085336286	2.5584023219635124
4	1.9350604108817466	1.6515257179069458
5	1.1566025716522217	1.2339142500017322
6	0.7603235217300729	0.9733756964424295
7	0.5469183948874401	0.8168670765671185
8	0.4393151060685265	0.749888935618897
9	0.35997664898800896	0.6912699227318728
>10	6.080651794543399	34.30086062465422
>50	1.942729959159711	26.757962462196698
>100	0.21288482668563136	7.142327046589029
>500	0.008436650979667294	1.205058799320518
>1k	0.0047807688884781326	1.913465122318976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3492	0.20918860341447082	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT	3369	0.20182027631825664	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3330	0.19948397748287164	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	3048	0.1825907397500879	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	2537	0.1519792344967103	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1995	0.11951067119469337	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1721	0.10309667424865529	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1689	0.10117971110167273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20763107085754748	0.0
2	0.0	0.0	0.0	0.6865723321114691	0.0
3	0.0	0.0	0.0	1.114174924085264	0.0
4	0.0	0.0	0.0	1.4102259200973817	0.0
5	0.0	0.0	0.0	3.023470218479884	0.0
6	0.0	0.0	0.0	3.4390917907850382	0.0
7	0.0	0.0	0.0	5.221747707282123	0.0
8	0.0	0.0	0.0	7.07059875744845	5.99050983432047E-5
9	0.0	0.0	0.0	8.951798560720107	5.99050983432047E-5
10	0.0	0.0	0.0	9.741767092571948	5.99050983432047E-5
11	5.99050983432047E-5	0.0	0.0	10.472189956670642	5.99050983432047E-5
12	5.99050983432047E-5	0.0	0.0	11.120482930940804	5.99050983432047E-5
13	5.99050983432047E-5	0.0	0.0	11.366573074934688	5.99050983432047E-5
14	5.99050983432047E-5	0.0	0.0	11.551500113520161	5.99050983432047E-5
15	5.99050983432047E-5	0.0	0.0	12.15001195106712	5.99050983432047E-5
16	5.99050983432047E-5	0.0	0.0	12.793692232764855	5.99050983432047E-5
17	5.99050983432047E-5	0.0	0.0	13.60486716943019	5.99050983432047E-5
18	5.99050983432047E-5	0.0	0.0	14.023963237439249	5.99050983432047E-5
19	5.99050983432047E-5	0.0	0.0	14.575209952393418	5.99050983432047E-5
20	5.99050983432047E-5	0.0	0.0	15.157068172600965	5.99050983432047E-5
21	5.99050983432047E-5	0.0	0.0	15.7072965008833	5.99050983432047E-5
22	5.99050983432047E-5	0.0	0.0	16.299997543890967	5.99050983432047E-5
23	1.198101966864094E-4	0.0	0.0	16.739461345336718	5.99050983432047E-5
24	1.797152950296141E-4	0.0	0.0	17.119379479029323	5.99050983432047E-5
25	1.797152950296141E-4	0.0	0.0	17.485100104414588	5.99050983432047E-5
26	1.797152950296141E-4	0.0	0.0	17.83045299636316	5.99050983432047E-5
27	1.797152950296141E-4	0.0	0.0	18.188865199750555	5.99050983432047E-5
28	2.396203933728188E-4	0.0	0.0	18.547756643924693	5.99050983432047E-5
29	2.396203933728188E-4	0.0	0.0	19.01381830903483	5.99050983432047E-5
30	2.9952549171602345E-4	0.0	0.0	19.440881755123534	5.99050983432047E-5
31	3.594305900592282E-4	0.0	0.0	19.848595854447385	5.99050983432047E-5
32	3.594305900592282E-4	0.0	0.0	20.22647721479632	5.99050983432047E-5
33	3.594305900592282E-4	0.0	0.0	20.589202585264424	5.99050983432047E-5
34	4.193356884024329E-4	0.0	0.0	21.063411343749234	5.99050983432047E-5
35	4.193356884024329E-4	0.0	0.0	21.440873368409765	5.99050983432047E-5
36	4.193356884024329E-4	0.0	0.0	21.83720549904841	5.99050983432047E-5
37	4.193356884024329E-4	0.0	0.0	22.19897238794302	5.99050983432047E-5
38	4.193356884024329E-4	0.0	0.0	22.58613903853515	5.99050983432047E-5
39	4.193356884024329E-4	0.0	0.0	22.981332972305275	5.99050983432047E-5
40	4.193356884024329E-4	0.0	0.0	23.38329618218818	5.99050983432047E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	260	0.0	46.0	14
ACGCGCC	20	6.31228E-4	46.0	31
ATTACGG	155	0.0	44.51613	1
TACGGGA	350	0.0	44.028572	3
ACGGGTA	100	0.0	43.7	4
TACGCGG	170	0.0	43.294117	1
TATACGG	155	0.0	43.032257	1
CATGCGG	360	0.0	42.805557	1
AACACGT	245	0.0	42.2449	40
TCTACGG	110	0.0	41.818184	1
CTAACGG	160	0.0	41.6875	1
CTTAATC	1110	0.0	41.648647	10
AGTACGG	205	0.0	41.512196	1
AACGGGA	435	0.0	41.24138	3
CGGTCTA	95	0.0	41.157894	30
CACGAGG	185	0.0	41.027027	1
CGTTTTT	2400	0.0	41.01667	1
AACGCAA	1010	0.0	40.990097	17
CAACGCA	1025	0.0	40.839024	16
GCTACGA	295	0.0	40.54237	10
>>END_MODULE