##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544885_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1064178
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8944584458615	34.0	33.0	34.0	31.0	34.0
2	33.27672062380542	34.0	33.0	34.0	31.0	34.0
3	33.41000565694837	34.0	34.0	34.0	31.0	34.0
4	36.628800820915295	37.0	37.0	37.0	35.0	37.0
5	36.63245716412104	37.0	37.0	37.0	35.0	37.0
6	36.440228984248876	37.0	37.0	37.0	35.0	37.0
7	36.232322036351064	37.0	37.0	37.0	35.0	37.0
8	36.36574708366457	37.0	37.0	37.0	35.0	37.0
9	38.24974111473832	39.0	39.0	39.0	37.0	39.0
10	38.132054036072915	39.0	39.0	39.0	35.0	39.0
11	37.743646269703	39.0	37.0	39.0	35.0	39.0
12	37.758525359479336	39.0	37.0	39.0	35.0	39.0
13	37.75782528862653	39.0	37.0	39.0	35.0	39.0
14	39.065021077300976	41.0	38.0	41.0	35.0	41.0
15	39.08563229083856	41.0	38.0	41.0	35.0	41.0
16	39.11267851806747	41.0	38.0	41.0	35.0	41.0
17	39.15875163741404	41.0	38.0	41.0	35.0	41.0
18	39.16417554206157	41.0	38.0	41.0	35.0	41.0
19	39.14003296441009	41.0	38.0	41.0	35.0	41.0
20	39.07994339292863	41.0	38.0	41.0	35.0	41.0
21	39.03259323158344	41.0	38.0	41.0	35.0	41.0
22	38.99190079103308	41.0	37.0	41.0	35.0	41.0
23	38.95076481566054	41.0	37.0	41.0	35.0	41.0
24	38.89262792502758	41.0	37.0	41.0	35.0	41.0
25	38.84182533373176	41.0	37.0	41.0	35.0	41.0
26	38.77587208155027	41.0	36.0	41.0	35.0	41.0
27	38.837219901181946	41.0	37.0	41.0	35.0	41.0
28	38.840736230217125	41.0	37.0	41.0	35.0	41.0
29	38.84627947580198	41.0	36.0	41.0	35.0	41.0
30	38.7899947189286	41.0	36.0	41.0	35.0	41.0
31	38.747026343337296	41.0	36.0	41.0	35.0	41.0
32	38.67744305933782	41.0	36.0	41.0	35.0	41.0
33	38.604500374937274	41.0	36.0	41.0	35.0	41.0
34	38.491884816261944	41.0	36.0	41.0	35.0	41.0
35	38.408618670936626	40.0	36.0	41.0	35.0	41.0
36	38.36056561966137	40.0	35.0	41.0	35.0	41.0
37	38.265251677820814	40.0	35.0	41.0	35.0	41.0
38	38.19587700553855	40.0	35.0	41.0	35.0	41.0
39	38.109599146007525	40.0	35.0	41.0	35.0	41.0
40	38.02233930789774	40.0	35.0	41.0	35.0	41.0
41	37.956750656375156	40.0	35.0	41.0	35.0	41.0
42	37.917533532924004	40.0	35.0	41.0	35.0	41.0
43	37.86562680303483	40.0	35.0	41.0	35.0	41.0
44	37.740486084094954	40.0	35.0	41.0	35.0	41.0
45	37.737511957586044	40.0	35.0	41.0	35.0	41.0
46	37.683255056954756	40.0	35.0	41.0	35.0	41.0
47	37.57859399461368	39.0	35.0	41.0	35.0	41.0
48	37.49087370721816	39.0	35.0	41.0	35.0	41.0
49	37.429356742950894	39.0	35.0	41.0	35.0	41.0
50	37.36210389615271	39.0	35.0	41.0	35.0	41.0
51	37.275735826149386	39.0	35.0	41.0	34.0	41.0
52	36.788096540240446	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	9.0
15	8.0
16	20.0
17	43.0
18	97.0
19	174.0
20	262.0
21	424.0
22	664.0
23	1084.0
24	1646.0
25	2601.0
26	3836.0
27	4596.0
28	4673.0
29	4896.0
30	5471.0
31	6773.0
32	9044.0
33	13386.0
34	45394.0
35	153810.0
36	42739.0
37	70196.0
38	123095.0
39	566553.0
40	2678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.71231128626978	21.475636594629847	22.814040508260835	21.99801161083954
2	27.73163887996181	24.627646878623686	25.429674358988812	22.211039882425684
3	28.39543760536301	24.86210013738303	24.410108083422134	22.332354173831824
4	26.55674144738944	27.11585843721633	21.67804634187138	24.64935377352285
5	25.54751178844141	36.62958640377831	18.90050348719857	18.922398320581706
6	87.66945003561433	3.1849934879315303	6.214279941889421	2.9312765345647063
7	86.42360582534126	3.2021898592152818	5.540144599869571	4.834059715573899
8	75.46340931686241	6.21926031171477	10.780809225524301	7.536521145898524
9	48.99800597268502	21.954315913315252	14.489587268295342	14.558090845704383
10	34.560759572176835	22.751269054613044	26.44087737201859	16.24709400119153
11	34.01724147651991	18.21236672812255	26.553170616193906	21.217221179163637
12	22.50741887165493	26.77155513457335	29.5098188460953	21.211207147676422
13	17.5367278782309	28.694729641093875	29.669566557474408	24.09897592320082
14	15.593256015441026	24.237392616648716	38.1516062162533	22.01774515165696
15	18.291488829876204	20.16692696146697	39.233380130015846	22.308204078640976
16	19.976169400231917	22.440324832875703	28.33266615171522	29.25083961517716
17	19.68608635021585	24.322246842163622	31.584565739942	24.40710106767853
18	23.943456827711156	26.566232340830197	26.479216822749578	23.01109400870907
19	26.294849169969687	23.742550588341423	25.496204582316118	24.466395659372775
20	22.991078560165686	28.766428172730503	26.146941583081023	22.095551684022784
21	21.891920336635412	24.10536583165598	24.589119489408727	29.413594342299877
22	20.690993424032445	28.433589117610026	24.598140536639548	26.277276921717984
23	20.61318689166662	22.736233975895008	33.04935828404646	23.601220848391904
24	21.055312175218805	23.327958292691637	30.100415531988066	25.516314000101488
25	20.04307550052717	27.215935679933246	27.979717678809372	24.761271140730216
26	20.73515896776667	25.974038177823633	26.94004198545732	26.35076086895237
27	22.160766337962258	24.76239877163407	31.727117080037363	21.34971781036631
28	20.769081864124235	24.574554256900633	28.655168590217052	26.001195288758083
29	22.19290381872206	25.4504415614681	26.53738378353997	25.81927083626987
30	24.82845914875143	24.637325710548424	24.314353425836654	26.21986171486349
31	26.780482212562184	24.418377376717054	24.200650643031523	24.60048976768924
32	25.89237890653631	24.145866574952688	25.06263049978481	24.89912401872619
33	20.911069388767668	24.578125088096165	28.112966063947948	26.397839459188216
34	23.618135311949693	23.68344393513115	29.414534034719757	23.283886718199398
35	28.234938140047998	24.34451755251471	25.60784004179752	21.81270426563977
36	25.174641836234162	29.068163408753044	24.13750331241578	21.61969144259701
37	24.691639932417324	29.1197525226043	25.47543737983683	20.713170165141545
38	25.491036274006788	26.962124757324435	23.804852195779276	23.741986772889497
39	29.875923012879426	22.821558047619853	25.7211669476347	21.581351991866022
40	21.79353454027428	21.702478344788183	28.803921900283598	27.70006521465394
41	22.935260830424987	25.002020338702735	24.98050138228755	27.08221744858473
42	23.717836677698656	21.858561255729775	27.875035943235062	26.54856612333651
43	24.309748932979257	25.743249719501815	27.446066353561154	22.500934993957777
44	20.840122611066946	27.020667595082777	27.412331395687563	24.726878398162714
45	22.72148080490294	26.2289767313363	26.5425520918493	24.506990371911467
46	24.815867270325075	22.254171764498047	27.811042889441428	25.11891807573545
47	23.859542294616126	21.1180836288666	29.604727780502888	25.41764629601439
48	22.728152621084067	21.141763877847502	27.803807257808373	28.326276243260057
49	22.205119820180457	23.82618321371049	29.288709219698205	24.679987746410845
50	21.950087297425807	24.027371360806182	28.00828432837364	26.014257013394378
51	21.61565076519154	21.950557143635745	29.773684477596795	26.660107613575924
52	23.327582415723686	20.651150465429655	31.00881619428329	25.012450924563375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	451.5
2	744.0
3	2477.5
4	4211.0
5	2858.0
6	1505.0
7	1492.0
8	1479.0
9	1484.0
10	1489.0
11	1486.5
12	1484.0
13	1481.0
14	1475.0
15	1472.0
16	1419.5
17	1367.0
18	1424.0
19	1481.0
20	1558.5
21	1636.0
22	1877.5
23	2119.0
24	2475.5
25	2832.0
26	3775.5
27	4719.0
28	7008.5
29	9298.0
30	9312.5
31	9327.0
32	10911.0
33	12495.0
34	14489.0
35	16483.0
36	17103.5
37	17724.0
38	21222.0
39	28200.0
40	31680.0
41	37791.0
42	43902.0
43	55029.0
44	66156.0
45	75568.5
46	84981.0
47	96590.5
48	108200.0
49	112451.5
50	116703.0
51	105715.0
52	94727.0
53	83660.0
54	72593.0
55	63827.0
56	55061.0
57	50556.0
58	46051.0
59	46207.0
60	46363.0
61	45887.0
62	45411.0
63	43995.5
64	35623.0
65	28666.0
66	23687.5
67	18709.0
68	15864.0
69	13019.0
70	11515.5
71	10012.0
72	8878.0
73	7744.0
74	6963.0
75	6182.0
76	4790.5
77	3399.0
78	2725.0
79	2051.0
80	1555.0
81	1059.0
82	815.5
83	572.0
84	324.5
85	77.0
86	49.0
87	21.0
88	25.0
89	17.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1064178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.618831260202985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8145806731252	18.398056242870293
2	10.402073420139232	5.32977927413579
3	3.5095990110951782	2.6973547456866784
4	1.7353498165677355	1.778305365122921
5	1.13634375552141	1.4555899463144177
6	0.7574557185768915	1.1643078144778312
7	0.6319092943904825	1.1332144309320593
8	0.5429063462463352	1.1126900859662554
9	0.5213724886014588	1.202125842827359
>10	8.414415751871655	51.25399411882907
>50	0.4182766720909184	7.009239431983359
>100	0.10613538506622196	4.588421378762509
>500	0.006264935924047495	1.1190649918203757
>1k	0.0033167307833192623	1.7578563302711192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3211	0.3017352360225451	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2986	0.2805921565753098	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT	2750	0.2584154154662096	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2203	0.20701424009893082	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	2104	0.19771128514214728	Illumina Single End Adapter 1 (95% over 22bp)
TGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTTG	1583	0.14875331006654902	Illumina Single End Adapter 1 (95% over 24bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1364	0.12817404607123997	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1316	0.12366352245582976	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1102	0.10355410467045928	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2627380005976444	0.0
2	0.0	0.0	0.0	0.8939293990291098	0.0
3	0.0	0.0	0.0	1.4124516763173078	0.0
4	0.0	0.0	0.0	1.7757367658418046	0.0
5	0.0	0.0	0.0	3.685285732274112	0.0
6	0.0	0.0	0.0	4.180221729823394	0.0
7	0.0	0.0	0.0	6.34536703446228	0.0
8	0.0	0.0	0.0	8.6310748765714	0.0
9	0.0	0.0	0.0	10.96160604710866	0.0
10	0.0	0.0	0.0	11.94349065663827	0.0
11	0.0	0.0	0.0	12.789213834527683	0.0
12	0.0	0.0	0.0	13.560137495794876	0.0
13	0.0	0.0	0.0	13.866759132400782	0.0
14	0.0	0.0	0.0	14.092943097865207	0.0
15	0.0	0.0	0.0	14.774408040760099	0.0
16	0.0	0.0	0.0	15.506898282054317	0.0
17	0.0	0.0	0.0	16.37855697073234	0.0
18	0.0	0.0	0.0	16.855262935336008	0.0
19	0.0	0.0	0.0	17.448960606214374	0.0
20	0.0	0.0	0.0	18.113886962519427	0.0
21	0.0	0.0	0.0	18.73699700613995	0.0
22	0.0	0.0	0.0	19.392338499762257	0.0
23	0.0	0.0	0.0	19.884831297019858	0.0
24	0.0	0.0	0.0	20.311921501854012	0.0
25	0.0	0.0	0.0	20.72942684400542	0.0
26	0.0	0.0	0.0	21.131709168954817	0.0
27	0.0	0.0	0.0	21.5104052141653	0.0
28	0.0	0.0	0.0	21.896900706460762	0.0
29	0.0	0.0	0.0	22.368720270481067	0.0
30	0.0	0.0	0.0	22.8097179231294	0.0
31	0.0	0.0	0.0	23.237371943415482	0.0
32	0.0	0.0	0.0	23.640687930026743	0.0
33	0.0	0.0	0.0	24.0295326533719	0.0
34	0.0	0.0	0.0	24.510373264623023	0.0
35	0.0	0.0	0.0	24.906923465811172	0.0
36	0.0	0.0	0.0	25.32649613128631	0.0
37	0.0	0.0	0.0	25.71590467008339	0.0
38	0.0	0.0	0.0	26.129369334829324	0.0
39	0.0	0.0	0.0	26.53503455249028	0.0
40	0.0	0.0	0.0	26.944364570588753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	75	0.0	46.0	1
TATCGGT	20	6.3112425E-4	46.0	8
TCGGTAC	20	6.3112425E-4	46.0	35
TACCGGT	45	3.092282E-10	46.0	40
ACGGGTA	90	0.0	43.444447	4
CGAATAT	90	0.0	43.444447	14
TACGGGA	250	0.0	43.24	3
ATAGCGG	145	0.0	42.827587	1
AACGCAA	720	0.0	42.48611	17
CTACCGG	65	0.0	42.46154	1
CGGTCTA	65	0.0	42.46154	30
CGCACTT	675	0.0	41.91111	34
CCGCACT	675	0.0	41.91111	33
CGGGATA	220	0.0	41.81818	5
AGTACGG	165	0.0	41.81818	1
TACGTGG	55	4.7293724E-11	41.81818	1
TCGGCGT	380	0.0	41.76316	4
GGACTAA	320	0.0	41.6875	7
GCGTAGG	195	0.0	41.28205	1
CAACGCA	750	0.0	41.09333	16
>>END_MODULE