FastQCFastQC Report
Thu 26 May 2016
SRR1544884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544884_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1182954
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT44780.3785438825178325No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT32920.27828639152494516Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT31230.26400012172916276No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.23060913611180145No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21860.18479163179633357No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG13110.11082425859331808No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12850.10862637093242847No Hit
GGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGG12850.10862637093242847No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT12070.10203270794975967No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC11910.10068016169690454No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT206.311529E-446.00000435
TACGAAC206.311529E-446.00000439
GCGATAT206.311529E-446.0000048
CTATTAG206.311529E-446.00000417
TAGATCG206.311529E-446.00000413
TTACGAA206.311529E-446.00000438
TAGTCCA206.311529E-446.00000446
CTAACGG750.046.01
GCTACGA253.4172175E-546.010
CGATAGA253.4172175E-546.09
ATACCGG301.8614501E-646.01
TACACGG1100.046.01
ATAACGG700.045.9999961
ATAGCGG1850.044.7567561
CATGCGG1200.044.0833321
ATTCGAA800.043.12500441
GTCGAAT750.042.9333329
TACGGGT700.042.7142833
ACGGGAT2050.042.6341444
TAGTAGG3650.042.2191771