##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544884_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1182954
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9295695352482	34.0	33.0	34.0	31.0	34.0
2	33.24717360100224	34.0	33.0	34.0	31.0	34.0
3	33.31295891471689	34.0	34.0	34.0	31.0	34.0
4	36.581713236524834	37.0	37.0	37.0	35.0	37.0
5	36.57690324391312	37.0	37.0	37.0	35.0	37.0
6	36.65931980448944	37.0	37.0	37.0	35.0	37.0
7	36.4877535390218	37.0	37.0	37.0	35.0	37.0
8	36.51074006258908	37.0	37.0	37.0	35.0	37.0
9	38.308422812721375	39.0	39.0	39.0	37.0	39.0
10	38.11100431631323	39.0	39.0	39.0	35.0	39.0
11	37.46528098303062	39.0	35.0	39.0	35.0	39.0
12	37.387922945439975	39.0	35.0	39.0	35.0	39.0
13	37.37846019371844	39.0	35.0	39.0	35.0	39.0
14	38.65663330949471	41.0	35.0	41.0	35.0	41.0
15	38.71258899331673	41.0	35.0	41.0	35.0	41.0
16	38.701103339605766	41.0	35.0	41.0	35.0	41.0
17	38.74763938411807	41.0	36.0	41.0	35.0	41.0
18	38.734206063802986	41.0	36.0	41.0	35.0	41.0
19	38.6603993054675	41.0	35.0	41.0	35.0	41.0
20	38.58457556253244	41.0	35.0	41.0	35.0	41.0
21	38.53161407797767	40.0	35.0	41.0	35.0	41.0
22	38.50023077820439	40.0	35.0	41.0	35.0	41.0
23	38.482510731609175	40.0	35.0	41.0	35.0	41.0
24	38.42258870590065	40.0	35.0	41.0	35.0	41.0
25	38.36831778750484	40.0	35.0	41.0	35.0	41.0
26	38.30694431059872	40.0	35.0	41.0	35.0	41.0
27	38.26527574191389	40.0	35.0	41.0	35.0	41.0
28	38.25066570635883	40.0	35.0	41.0	35.0	41.0
29	38.25796100271017	40.0	35.0	41.0	35.0	41.0
30	38.19307090554663	40.0	35.0	41.0	35.0	41.0
31	38.03609692346448	40.0	35.0	41.0	35.0	41.0
32	38.019169807109996	40.0	35.0	41.0	35.0	41.0
33	37.86018813918377	40.0	35.0	41.0	35.0	41.0
34	37.71934749787397	40.0	35.0	41.0	35.0	41.0
35	37.65199661187164	40.0	35.0	41.0	35.0	41.0
36	37.63853793131432	40.0	35.0	41.0	35.0	41.0
37	37.61332139711265	40.0	35.0	41.0	35.0	41.0
38	37.53788059383543	40.0	35.0	41.0	35.0	41.0
39	37.46761919736524	39.0	35.0	41.0	34.0	41.0
40	37.408941514209346	39.0	35.0	41.0	34.0	41.0
41	37.341506939407616	39.0	35.0	41.0	34.0	41.0
42	37.23440979108233	39.0	35.0	41.0	34.0	41.0
43	37.115166777406394	39.0	35.0	41.0	34.0	41.0
44	37.06697048236872	38.0	35.0	41.0	34.0	41.0
45	37.010911666894906	38.0	35.0	41.0	34.0	41.0
46	37.01652642452707	38.0	35.0	41.0	34.0	41.0
47	36.950474828268895	38.0	35.0	41.0	34.0	41.0
48	36.94140262427787	38.0	35.0	41.0	34.0	41.0
49	36.887833339250726	37.0	35.0	41.0	34.0	41.0
50	36.82324756499408	37.0	35.0	41.0	34.0	41.0
51	36.74167296446016	37.0	35.0	41.0	34.0	41.0
52	36.53163521151287	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	6.0
13	15.0
14	14.0
15	23.0
16	38.0
17	68.0
18	117.0
19	179.0
20	329.0
21	525.0
22	906.0
23	1400.0
24	2324.0
25	3538.0
26	5052.0
27	6102.0
28	6482.0
29	7057.0
30	8330.0
31	10294.0
32	13321.0
33	19231.0
34	63923.0
35	233918.0
36	50005.0
37	78683.0
38	131883.0
39	534904.0
40	4285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.324457248548974	22.085051489745165	23.360587140328366	21.2299041213775
2	29.149654170829976	23.51689076667394	24.937317934594244	22.396137127901845
3	29.03113730542354	23.852406771522816	24.763431206961556	22.353024716092087
4	26.99352637549727	26.479896935975532	21.419936869903648	25.10663981862355
5	25.035208469644637	35.52961484554767	20.319555959065188	19.115620725742506
6	87.19738890945887	3.0442434786137076	7.22428767306252	2.534079938864909
7	86.83676626479136	3.0716325402340243	5.676974759796239	4.414626435178375
8	75.41493583013371	5.976986425507669	11.53502164919346	7.073056095165155
9	52.65124425801848	18.069933404003873	14.577151774287081	14.701670563690557
10	41.12061838414681	20.412205377385764	22.81373578347087	15.65344045499656
11	38.12760259485999	16.763289189605004	25.22278972808748	19.886318487447525
12	22.08522055802677	29.5353834553161	28.68750602305753	19.6918899635996
13	16.575116192176534	31.959230874573315	31.24356483853134	20.222088094718814
14	16.138243752504327	22.047856467791647	42.05877827878346	19.755121500920577
15	18.766241121801862	17.632638293627647	42.71459414313659	20.886526441433904
16	19.6257842654913	20.324712541654197	27.53648916187781	32.51301403097669
17	21.214265305328865	22.913486069618937	32.01840477313573	23.853843851916473
18	24.822097900679147	25.979877493123148	26.915332295254085	22.282692310943624
19	27.421691798666725	23.08728826311082	24.79411710007321	24.696902838149242
20	22.89142265886924	28.766629978849558	26.541775927043652	21.80017143523755
21	22.223518412381207	22.781443741683955	26.193157130370242	28.801880715564593
22	21.391617932734494	27.35042951796942	24.030351137914067	27.227601411382015
23	21.72096294530472	21.556290439019605	33.99058627808012	22.732160337595545
24	21.483337475506232	20.893204638557375	32.12584766609691	25.497610219839483
25	19.015532303031225	27.380439137954642	28.851924926920237	24.752103632093895
26	20.471379275948177	26.692584834237003	27.139347768383214	25.696688121431606
27	21.85393514878854	25.77589661136443	32.60608612000129	19.764082119845742
28	20.67113345066672	24.134074528679896	29.762104020950943	25.43268799970244
29	23.57555746039153	25.71689178108363	27.43428738564644	23.273263372878404
30	29.8518792784842	23.27757461405938	23.368956020267905	23.50159008718851
31	29.83742394040681	22.723030650388772	26.045645054668228	21.393900354536186
32	28.126875601249075	25.95688420682461	23.369209622690317	22.547030569236
33	22.731399530328314	23.34900596303829	28.945335152508044	24.97425935412535
34	21.588836083228934	24.255127418310433	30.884971013243117	23.271065485217516
35	27.941576764607923	23.710135812550615	26.447266757625403	21.901020665216063
36	23.31189547522558	28.693846083617792	27.338594738256937	20.65566370289969
37	21.528309638413667	27.273418915697484	28.106249270893034	23.092022174995815
38	21.09202893772708	26.584719270571807	26.31421002000078	26.009041771700335
39	25.27393288327357	23.239280648275418	27.032919285111678	24.453867183339334
40	19.094994395386465	21.95960282479285	29.899049329052524	29.046353450768166
41	22.84704223494743	24.3548777044585	24.473394569864933	28.32468549072914
42	23.001993315040146	21.621973466423885	27.901930252571105	27.474102965964864
43	23.39456986493135	25.304280639822004	28.265765194589136	23.03538430065751
44	20.311694283970468	27.856281816537244	27.107647465581923	24.724376433910365
45	23.09946117938652	25.372330623168782	27.608005044997526	23.92020315244718
46	24.26755393700854	21.944133077025818	28.481496321919536	25.306816664046107
47	22.364267756818947	20.533089198734693	31.70351509864289	25.399127945803475
48	22.3336663978481	20.28354441508292	29.348393935858873	28.034395251210103
49	21.149512153473424	23.60632788764398	30.737205335118695	24.5069546237639
50	21.00521237512194	24.897586888416626	28.062798722520064	26.03440201394137
51	21.34884365748795	21.190680280044702	30.226957261229092	27.233518801238255
52	23.14392613744913	19.630180040813084	32.6432811419548	24.582612679782983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	319.0
1	534.5
2	750.0
3	2991.5
4	5233.0
5	3481.5
6	1730.0
7	1746.0
8	1762.0
9	1849.5
10	1937.0
11	1881.5
12	1826.0
13	1775.5
14	1598.0
15	1471.0
16	1443.0
17	1415.0
18	1312.5
19	1210.0
20	1539.0
21	1868.0
22	2248.0
23	2628.0
24	2768.0
25	2908.0
26	3390.5
27	3873.0
28	5449.5
29	7026.0
30	8048.0
31	9070.0
32	10370.5
33	11671.0
34	13223.5
35	14776.0
36	15844.0
37	16912.0
38	19727.5
39	27287.5
40	32032.0
41	45008.0
42	57984.0
43	79723.5
44	101463.0
45	114624.5
46	127786.0
47	130238.0
48	132690.0
49	120838.0
50	108986.0
51	97371.0
52	85756.0
53	76072.5
54	66389.0
55	62169.0
56	57949.0
57	54062.5
58	50176.0
59	50566.0
60	50956.0
61	51000.5
62	51045.0
63	47673.0
64	37566.5
65	30832.0
66	25519.5
67	20207.0
68	17736.0
69	15265.0
70	13499.5
71	11734.0
72	10192.5
73	8651.0
74	7493.0
75	6335.0
76	5632.5
77	4930.0
78	3914.0
79	2898.0
80	2011.5
81	1125.0
82	752.5
83	380.0
84	333.5
85	287.0
86	194.5
87	102.0
88	56.0
89	19.0
90	28.0
91	15.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1182954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.567512655986526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4762262578384	15.679056153639515
2	12.620793776467504	5.696398465580528
3	4.643262301689653	3.1436064227533906
4	2.2590474546741612	2.039243280953331
5	1.2639663199196822	1.4262287960764068
6	0.759558889245159	1.0284812907604317
7	0.5691398146650557	0.899084897933563
8	0.44554669883515574	0.8043904611836317
9	0.33578433102612326	0.6820035426102605
>10	5.882744130540361	35.94740800965223
>50	1.52931559063317	21.63835362589534
>100	0.20185763241692078	7.518482872261901
>500	0.007504001205089858	1.2559593716271318
>1k	0.0052528008435629	2.2413028090723506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4478	0.3785438825178325	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	3292	0.27828639152494516	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	3123	0.26400012172916276	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2728	0.23060913611180145	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2186	0.18479163179633357	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1311	0.11082425859331808	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1285	0.10862637093242847	No Hit
GGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGG	1285	0.10862637093242847	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1207	0.10203270794975967	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1191	0.10068016169690454	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2854717934932381	0.0
2	0.0	0.0	0.0	1.1232051288553908	0.0
3	0.0	0.0	0.0	1.770483044987379	0.0
4	0.0	0.0	0.0	2.186221949458728	0.0
5	0.0	0.0	0.0	4.51995597461947	0.0
6	0.0	0.0	0.0	4.961393257895066	0.0
7	0.0	0.0	0.0	6.938055072302051	0.0
8	8.453414080344628E-5	0.0	0.0	9.044223190419915	0.0
9	8.453414080344628E-5	0.0	0.0	10.878529511713896	0.0
10	8.453414080344628E-5	0.0	0.0	11.805277297342078	0.0
11	1.6906828160689256E-4	0.0	0.0	12.74749483073729	0.0
12	1.6906828160689256E-4	0.0	0.0	13.53095724770363	0.0
13	1.6906828160689256E-4	0.0	0.0	13.868924742635809	0.0
14	1.6906828160689256E-4	0.0	0.0	14.103084312661355	0.0
15	1.6906828160689256E-4	0.0	0.0	14.893985734018399	0.0
16	1.6906828160689256E-4	0.0	0.0	15.693002432892571	0.0
17	1.6906828160689256E-4	0.0	0.0	16.834128799598293	0.0
18	1.6906828160689256E-4	0.0	0.0	17.37244220823464	0.0
19	1.6906828160689256E-4	0.0	0.0	18.06731284563897	0.0
20	1.6906828160689256E-4	0.0	0.0	18.798279561166368	0.0
21	1.6906828160689256E-4	0.0	0.0	19.43220108305141	0.0
22	1.6906828160689256E-4	0.0	0.0	20.12462023037244	0.0
23	1.6906828160689256E-4	0.0	0.0	20.654142088365226	0.0
24	2.536024224103389E-4	0.0	0.0	21.12212309185311	0.0
25	2.536024224103389E-4	0.0	0.0	21.56812521873209	0.0
26	3.3813656321378513E-4	0.0	0.0	21.989950581341287	0.0
27	3.3813656321378513E-4	0.0	0.0	22.437051652050712	0.0
28	3.3813656321378513E-4	0.0	0.0	22.846365961821	0.0
29	3.3813656321378513E-4	0.0	0.0	23.395161603916975	0.0
30	3.3813656321378513E-4	0.0	0.0	23.916906320955846	0.0
31	3.3813656321378513E-4	0.0	0.0	24.396975706578615	0.0
32	3.3813656321378513E-4	0.0	0.0	24.8602227981815	0.0
33	3.3813656321378513E-4	0.0	0.0	25.28872635791417	0.0
34	3.3813656321378513E-4	0.0	0.0	25.852315474650748	0.0
35	3.3813656321378513E-4	0.0	0.0	26.2902868581534	0.0
36	4.2267070401723144E-4	0.0	0.0	26.74524960395755	0.0
37	4.2267070401723144E-4	0.0	0.0	27.205791603054724	0.0
38	4.2267070401723144E-4	0.0	0.0	27.647567022893536	0.0
39	4.2267070401723144E-4	0.0	0.0	28.1961090625671	0.0
40	4.2267070401723144E-4	0.0	0.0	28.67592484576746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	6.311529E-4	46.000004	35
TACGAAC	20	6.311529E-4	46.000004	39
GCGATAT	20	6.311529E-4	46.000004	8
CTATTAG	20	6.311529E-4	46.000004	17
TAGATCG	20	6.311529E-4	46.000004	13
TTACGAA	20	6.311529E-4	46.000004	38
TAGTCCA	20	6.311529E-4	46.000004	46
CTAACGG	75	0.0	46.0	1
GCTACGA	25	3.4172175E-5	46.0	10
CGATAGA	25	3.4172175E-5	46.0	9
ATACCGG	30	1.8614501E-6	46.0	1
TACACGG	110	0.0	46.0	1
ATAACGG	70	0.0	45.999996	1
ATAGCGG	185	0.0	44.756756	1
CATGCGG	120	0.0	44.083332	1
ATTCGAA	80	0.0	43.125004	41
GTCGAAT	75	0.0	42.93333	29
TACGGGT	70	0.0	42.714283	3
ACGGGAT	205	0.0	42.634144	4
TAGTAGG	365	0.0	42.219177	1
>>END_MODULE