FastQCFastQC Report
Thu 26 May 2016
SRR1544883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544883_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences709748
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT35180.4956688852944989No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT25590.36055050524975063Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA25000.35223769563281615No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT23200.3268765815472534No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.2362810462304931No Hit
TGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTTG15250.21486499433601786Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12630.1779504838336987No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC8740.12314229839323251No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT8330.11736560018485434No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC8220.11581575432406996No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC7900.11130711181996991No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7590.10693936439412298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA351.01883415E-746.00000430
CGTTGAT253.4158253E-546.024
ATATGCG206.309814E-446.032
CATTAGC206.309814E-446.032
GCCGATT206.309814E-446.027
GCCGATG206.309814E-446.08
TCAGCGA206.309814E-446.035
TACGATC206.309814E-446.010
TTGACGT206.309814E-446.024
AACGCTT206.309814E-446.031
ACGTAGG551.8189894E-1246.01
TCGGTAC206.309814E-446.035
GCATACG206.309814E-446.020
TAGTAGG1300.044.2307661
CATGCGG1150.044.01
GGACTAA1050.043.8095257
CGTTTTT10900.043.4678881
TAGCGGG5100.042.843142
TGCATGG1400.042.7142871
TACGGGT650.042.4615363
Produced by FastQC (version 0.11.5)