FastQCFastQC Report
Thu 26 May 2016
SRR1544882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544882_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences857191
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT26050.3038995976392659No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT23730.27683445113166144Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT21880.255252329994132No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.23717001228431003No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT14000.16332415995968227No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10120.1180600356279989No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9550.11141040911535469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT700.046.0000043
GTAATCG351.0191616E-746.00000432
CGACGCC206.310552E-446.035
TATGCAT206.310552E-446.026
TAGTTCG253.4164237E-546.027
CTAACGG501.6370905E-1146.01
ACTACGG453.092282E-1046.01
GCGATCT253.4164237E-546.08
ACGTAGG750.046.01
AATACGG301.8608443E-646.01
GTAACGC301.8608443E-646.033
CGCACTT3650.044.73972334
GGCGATA1000.043.77
GACTAAT2850.043.578958
CCGCACT3750.043.54666533
ATCAACG4350.043.3563214
GCTACGA1650.043.2121210
TACGGGA800.043.1253
CTACGAA1550.043.03225711
AGTACGG750.042.933331