##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544882_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	857191
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91993266378205	34.0	33.0	34.0	31.0	34.0
2	33.254901183050215	34.0	33.0	34.0	31.0	34.0
3	33.329172844791884	34.0	34.0	34.0	31.0	34.0
4	36.59613318385284	37.0	37.0	37.0	35.0	37.0
5	36.59178409479334	37.0	37.0	37.0	35.0	37.0
6	36.66838662561786	37.0	37.0	37.0	35.0	37.0
7	36.494922368526964	37.0	37.0	37.0	35.0	37.0
8	36.53540109497183	37.0	37.0	37.0	35.0	37.0
9	38.32912501414504	39.0	39.0	39.0	37.0	39.0
10	38.15177947505282	39.0	39.0	39.0	35.0	39.0
11	37.56178611301332	39.0	35.0	39.0	35.0	39.0
12	37.49188453915172	39.0	35.0	39.0	35.0	39.0
13	37.479397240521656	39.0	35.0	39.0	35.0	39.0
14	38.81278851504507	41.0	36.0	41.0	35.0	41.0
15	38.87339461100268	41.0	36.0	41.0	35.0	41.0
16	38.87171003895281	41.0	36.0	41.0	35.0	41.0
17	38.923407968585764	41.0	36.0	41.0	35.0	41.0
18	38.90187484469622	41.0	36.0	41.0	35.0	41.0
19	38.837935769274296	41.0	36.0	41.0	35.0	41.0
20	38.76274599243342	41.0	35.0	41.0	35.0	41.0
21	38.712991620303995	41.0	35.0	41.0	35.0	41.0
22	38.67519257668361	41.0	35.0	41.0	35.0	41.0
23	38.65919964162013	41.0	35.0	41.0	35.0	41.0
24	38.59308018866274	41.0	35.0	41.0	35.0	41.0
25	38.54706477319524	41.0	35.0	41.0	35.0	41.0
26	38.485598892195554	40.0	35.0	41.0	35.0	41.0
27	38.4456684682877	40.0	35.0	41.0	35.0	41.0
28	38.457502470277916	40.0	36.0	41.0	35.0	41.0
29	38.47459084381427	40.0	36.0	41.0	35.0	41.0
30	38.405746210587836	40.0	35.0	41.0	35.0	41.0
31	38.334335054847756	40.0	35.0	41.0	35.0	41.0
32	38.25727054997078	40.0	35.0	41.0	35.0	41.0
33	38.17861596773648	40.0	35.0	41.0	35.0	41.0
34	38.06245165896515	40.0	35.0	41.0	35.0	41.0
35	37.99415416167459	40.0	35.0	41.0	35.0	41.0
36	37.915378252921464	40.0	35.0	41.0	35.0	41.0
37	37.85401736602461	40.0	35.0	41.0	35.0	41.0
38	37.79043060414774	40.0	35.0	41.0	35.0	41.0
39	37.659900768906816	40.0	35.0	41.0	35.0	41.0
40	37.57131257794354	40.0	35.0	41.0	35.0	41.0
41	37.523025790051456	40.0	35.0	41.0	35.0	41.0
42	37.45527309549447	39.0	35.0	41.0	35.0	41.0
43	37.364782178067664	39.0	35.0	41.0	34.0	41.0
44	37.300126809544196	39.0	35.0	41.0	34.0	41.0
45	37.26043787207285	39.0	35.0	41.0	34.0	41.0
46	37.27941497285902	39.0	35.0	41.0	34.0	41.0
47	37.21871554881001	39.0	35.0	41.0	34.0	41.0
48	37.18289389412628	39.0	35.0	41.0	34.0	41.0
49	37.1069283275256	39.0	35.0	41.0	34.0	41.0
50	37.02956283955385	38.0	35.0	41.0	34.0	41.0
51	36.95322396058755	38.0	35.0	41.0	34.0	41.0
52	36.75442929288805	37.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	5.0
14	8.0
15	6.0
16	22.0
17	39.0
18	67.0
19	113.0
20	217.0
21	355.0
22	547.0
23	919.0
24	1749.0
25	2644.0
26	3682.0
27	4428.0
28	4611.0
29	4904.0
30	5572.0
31	6681.0
32	8747.0
33	12398.0
34	40509.0
35	158432.0
36	34274.0
37	52287.0
38	90227.0
39	419641.0
40	4100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37634202878939	22.346128225797983	23.800063229781927	20.477466515630706
2	26.79892812687021	24.08739709119671	26.395400791655536	22.718273990277545
3	27.522687475720115	24.502590437837075	25.362142159682033	22.61257992676078
4	26.78201241030295	27.20957172905455	22.12424068848133	23.88417517216116
5	24.47669189247204	35.78525672808044	20.27541119773773	19.462640181709794
6	87.6370610517376	3.2521340051400447	6.736071657308582	2.37473328581378
7	87.4253229443613	2.7709110338302665	5.281903333096125	4.521862688712317
8	77.12376821501859	5.575886821023553	10.562406744821166	6.7379382191366926
9	50.105402413231126	19.37957818035887	15.26975901520198	15.245260391208026
10	39.10458695903247	22.52135171741187	23.08003700458824	15.294024318967416
11	37.90858746767057	17.271413255622143	25.351176108941882	19.46882316776541
12	22.075243440493423	30.344345659252138	28.14705240722313	19.433358493031307
13	16.259853404900422	32.119212637556856	29.34410183961334	22.276832117929377
14	14.886297219639497	24.10291288639288	40.872337670367514	20.13845222360011
15	17.865563217532614	18.183578688996967	42.588174630858234	21.362683462612182
16	17.633992890732635	22.09554230037413	28.05360765570334	32.2168571531899
17	20.538713075615586	22.945761213078534	32.269237544491254	24.24628816681463
18	23.911706959125794	27.62604833695174	25.96107518627704	22.501169517645426
19	26.947436452319263	23.050871976024013	25.98184068661477	24.019850885041958
20	22.596014190536298	28.85401269962004	26.474846329464498	22.07512678037917
21	21.935017983156612	23.986602752478735	25.263214382792164	28.815164881572485
22	20.233063576262467	28.380139315508444	23.46781522437823	27.918981883850858
23	20.38647162651031	21.719780072352606	35.2194551739344	22.674293127202688
24	20.225247348607255	22.276948778043636	31.74123386736445	25.756570005984663
25	18.368251649865666	28.866611991959783	28.398688273675294	24.366448084499254
26	19.42239244229116	28.207599006522464	27.42060987574531	24.949398675441063
27	20.028208415627322	27.317249014513685	33.035461174930674	19.619081394928315
28	18.903254933847883	25.24594868588214	29.756262023283025	26.094534356986948
29	20.634374369306258	29.08733292813387	27.152058292725894	23.12623440983398
30	25.50563410021804	25.205117645892223	25.246298666224913	24.04294958766483
31	26.227176906897064	26.851775158628588	25.263214382792164	21.65783355168218
32	23.247210948318404	29.937901821181047	24.56407031805047	22.250816912450084
33	21.28370456526025	26.69440066449601	27.713660082758686	24.308234687485054
34	19.94654633564748	27.618932069982073	29.68801585644273	22.74650573792772
35	26.98336776751039	25.61494462727677	24.549604463882613	22.85208314133023
36	24.75387632394647	30.13400747324692	25.144454386478625	19.967661816327983
37	22.230051412112353	28.97615583924703	25.481602116681113	23.312190631959506
38	22.416474274694906	27.613099064269225	24.323750482681223	25.64667617835465
39	26.82156018903605	23.40656866439335	25.75470344415655	24.017167702414046
40	19.171806516867303	22.969793196615456	30.082793683088134	27.77560660342911
41	21.789192840335467	25.950925756336684	25.09289061597707	27.166990787350777
42	22.812185382254363	22.7056746979378	28.267095664793494	26.21504425501434
43	23.464665401293296	27.001333425105955	27.029681832870388	22.504319340730362
44	19.222320346340545	29.953417616377216	26.2159775359284	24.608284501353843
45	22.96827661513012	27.287734005606683	26.012172316321568	23.731817062941634
46	23.27602599653986	24.815472864274124	27.299516677146634	24.60898446203938
47	22.88521461377919	21.419030297798276	30.74378989046782	24.951965197954713
48	22.62051281453025	21.019002766011308	28.150902190993605	28.209582228464836
49	21.06846665445624	25.030710775078134	29.816808622582364	24.08401394788326
50	20.57021130646495	25.40810624469926	27.519887632977948	26.501794815857842
51	20.488549226485112	22.825134654936882	29.91585305958649	26.770463058991524
52	23.042589107911773	20.461834060320278	31.26374401971089	25.231832812057053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	351.0
2	581.0
3	2226.5
4	3872.0
5	2640.0
6	1408.0
7	1386.0
8	1364.0
9	1444.0
10	1524.0
11	1439.0
12	1354.0
13	1341.5
14	1217.5
15	1106.0
16	1054.5
17	1003.0
18	1146.0
19	1289.0
20	1328.5
21	1368.0
22	1365.5
23	1363.0
24	1752.0
25	2141.0
26	2508.0
27	2875.0
28	5139.5
29	7404.0
30	7924.5
31	8445.0
32	9161.5
33	9878.0
34	11241.5
35	12605.0
36	13766.5
37	14928.0
38	17241.5
39	23145.5
40	26736.0
41	35837.0
42	44938.0
43	60533.5
44	76129.0
45	88568.5
46	101008.0
47	100552.0
48	100096.0
49	88425.0
50	76754.0
51	67540.0
52	58326.0
53	51910.0
54	45494.0
55	42336.5
56	39179.0
57	36309.0
58	33439.0
59	33892.5
60	34346.0
61	35093.0
62	35840.0
63	32527.0
64	24640.5
65	20067.0
66	16005.5
67	11944.0
68	9947.0
69	7950.0
70	7101.5
71	6253.0
72	5768.0
73	5283.0
74	4465.5
75	3648.0
76	3149.0
77	2650.0
78	1982.0
79	1314.0
80	962.0
81	610.0
82	476.5
83	343.0
84	209.5
85	76.0
86	46.5
87	17.0
88	17.0
89	11.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	857191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.173236639162294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.07197034742751	17.891115279673183
2	11.23180815535464	5.654819291608306
3	4.066307018189347	3.070863264491005
4	1.9616555319446192	1.9752487564065473
5	1.1419955262293462	1.4373861811318
6	0.768391001533993	1.1605733107810874
7	0.5556501803684073	0.9791259435304989
8	0.46651807987500604	0.9395016016912915
9	0.39460548108494997	0.8940147439015734
>10	7.637384881037688	49.48430303018468
>50	0.5848808602779579	9.422706648866008
>100	0.11001330465759364	4.711937583645291
>500	0.006034485065606401	1.0216365016816482
>1k	0.002785146953356801	1.3567678624071389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2605	0.3038995976392659	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	2373	0.27683445113166144	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2188	0.255252329994132	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.23717001228431003	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1400	0.16332415995968227	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1012	0.1180600356279989	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	955	0.11141040911535469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2869838810720131	0.0
2	0.0	0.0	0.0	1.0268423256893737	0.0
3	0.0	0.0	0.0	1.6316083579972258	0.0
4	0.0	0.0	0.0	2.029419347613309	0.0
5	0.0	0.0	0.0	4.2442116167808575	0.0
6	0.0	0.0	0.0	4.676204019874217	0.0
7	0.0	0.0	0.0	6.734321755594728	0.0
8	0.0	0.0	0.0	8.919365695626762	0.0
9	0.0	0.0	0.0	10.99276590631493	0.0
10	0.0	0.0	0.0	11.91344752803051	0.0
11	0.0	0.0	0.0	12.812080388151532	0.0
12	0.0	0.0	0.0	13.55088889174058	0.0
13	0.0	0.0	0.0	13.858288292807554	0.0
14	0.0	0.0	0.0	14.086242156065568	0.0
15	0.0	0.0	0.0	14.853399067419046	0.0
16	0.0	0.0	0.0	15.695101791782696	0.0
17	0.0	0.0	0.0	16.78587386008486	0.0
18	0.0	0.0	0.0	17.320877144067076	0.0
19	0.0	0.0	0.0	17.973823803563032	0.0
20	0.0	0.0	0.0	18.725114939377573	0.0
21	0.0	0.0	0.0	19.415392835435743	0.0
22	0.0	0.0	0.0	20.13716896234328	0.0
23	0.0	0.0	0.0	20.673455507582325	0.0
24	1.166601142569159E-4	0.0	0.0	21.164244608261168	0.0
25	1.166601142569159E-4	0.0	0.0	21.605336500266567	0.0
26	1.166601142569159E-4	0.0	0.0	22.025662891934235	0.0
27	1.166601142569159E-4	0.0	0.0	22.444589362230822	0.0
28	1.166601142569159E-4	0.0	0.0	22.87168204052539	0.0
29	1.166601142569159E-4	0.0	0.0	23.41333495102025	0.0
30	1.166601142569159E-4	0.0	0.0	23.921973049180405	0.0
31	1.166601142569159E-4	0.0	0.0	24.389896767464894	0.0
32	1.166601142569159E-4	0.0	0.0	24.84440457260984	0.0
33	1.166601142569159E-4	0.0	0.0	25.266364205877103	0.0
34	1.166601142569159E-4	0.0	0.0	25.79833432688864	0.0
35	1.166601142569159E-4	0.0	0.0	26.23184331146734	0.0
36	1.166601142569159E-4	0.0	0.0	26.70501673489339	0.0
37	1.166601142569159E-4	0.0	0.0	27.16640748677949	0.0
38	1.166601142569159E-4	0.0	0.0	27.603999575357186	0.0
39	2.333202285138318E-4	0.0	0.0	28.089188990551698	0.0
40	2.333202285138318E-4	0.0	0.0	28.597243788140567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	70	0.0	46.000004	3
GTAATCG	35	1.0191616E-7	46.000004	32
CGACGCC	20	6.310552E-4	46.0	35
TATGCAT	20	6.310552E-4	46.0	26
TAGTTCG	25	3.4164237E-5	46.0	27
CTAACGG	50	1.6370905E-11	46.0	1
ACTACGG	45	3.092282E-10	46.0	1
GCGATCT	25	3.4164237E-5	46.0	8
ACGTAGG	75	0.0	46.0	1
AATACGG	30	1.8608443E-6	46.0	1
GTAACGC	30	1.8608443E-6	46.0	33
CGCACTT	365	0.0	44.739723	34
GGCGATA	100	0.0	43.7	7
GACTAAT	285	0.0	43.57895	8
CCGCACT	375	0.0	43.546665	33
ATCAACG	435	0.0	43.35632	14
GCTACGA	165	0.0	43.21212	10
TACGGGA	80	0.0	43.125	3
CTACGAA	155	0.0	43.032257	11
AGTACGG	75	0.0	42.93333	1
>>END_MODULE