FastQCFastQC Report
Thu 26 May 2016
SRR1544881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544881_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences549943
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT23250.4227710871853992No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT18990.3453085137914293Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT16050.29184842792798527No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.2462073342146368No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12210.2220230096573645No Hit
TGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTTG11400.2072942104909054Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6510.11837590441191179No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT6140.11164793442229466No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC6050.11001140118157701No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC5940.10801119388736652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG351.0182703E-746.0000041
GCCGATG351.0182703E-746.0000048
CTAACGG351.0182703E-746.0000041
CCGTAGG351.0182703E-746.0000041
ACTACGG351.0182703E-746.0000041
TATCCGG351.0182703E-746.0000041
CTCACGT253.4148146E-546.044
CGACGGT405.6024874E-946.027
TTCACGG301.8596202E-646.01
ATAGCGG750.046.01
ATTCCGG301.8596202E-646.01
AACGGAC206.3085684E-446.03
CTACGGA253.4148146E-546.034
GTATGAT206.3085684E-446.033
CTACGAA405.6024874E-946.011
AGACCGG253.4148146E-546.01
ATAACGG253.4148146E-546.01
ACGTAGG206.3085684E-446.01
GCTACGA405.6024874E-946.010
CGTCATT253.4148146E-546.037
Produced by FastQC (version 0.11.5)