FastQCFastQC Report
Thu 26 May 2016
SRR1544880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544880_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3320582
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT160180.4823853167908517TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT134960.4064347755905441TruSeq Adapter, Index 20 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC123500.37192275330047564No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT113020.3403620208746539TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC97920.2948880648030978No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG88230.2657064333902912No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78010.2349286962345757No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC50220.15123854794129463TruSeq Adapter, Index 20 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC42100.12678500335182205No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC38610.11627479761078029TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT38240.11516053511101366TruSeq Adapter, Index 22 (95% over 23bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC37650.11338373815192639No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT36190.10898691855825275No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA35630.10730046720725463No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT35290.1062765503155772No Hit
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC33800.10178938511381438TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT206.313186E-446.018
CTAACGG2600.043.346151
AATACGG1400.041.071431
CGTTTTT52450.040.95711
CGACGGT3550.040.81690227
TAGTAGG5300.040.7924541
CGGTCTA3400.040.58823430
TCTAACG402.9128023E-740.2546
ATCAACG35800.040.08938614
TCAACGC36000.039.80277615
CACTTAC31900.039.7993736
CCGCACT32150.039.7045133
CACGACG3650.039.6986325
CGCACTT32150.039.5614334
CATACGA6400.039.5312517
CCCGCAC32500.039.4184632
TACGGGA6950.039.3812943
AACGCAA36050.039.36477317
CTTAATC40200.039.36318210
CGCAAGC36150.039.2558819
Produced by FastQC (version 0.11.5)