##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544880_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3320582
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86683930708532	34.0	33.0	34.0	31.0	34.0
2	33.19799842316799	34.0	33.0	34.0	31.0	34.0
3	33.26347941415089	34.0	34.0	34.0	31.0	34.0
4	36.55387429071169	37.0	37.0	37.0	35.0	37.0
5	36.52426652918073	37.0	37.0	37.0	35.0	37.0
6	36.62260772358581	37.0	37.0	37.0	35.0	37.0
7	36.607267039332264	37.0	37.0	37.0	35.0	37.0
8	36.49011980429937	37.0	37.0	37.0	35.0	37.0
9	38.302296705818435	39.0	39.0	39.0	37.0	39.0
10	38.12388972776459	39.0	39.0	39.0	35.0	39.0
11	37.63148508303665	39.0	37.0	39.0	35.0	39.0
12	37.55698067386982	39.0	37.0	39.0	35.0	39.0
13	37.48084100919658	39.0	37.0	39.0	35.0	39.0
14	38.870816019601385	41.0	38.0	41.0	35.0	41.0
15	38.94203034287363	41.0	38.0	41.0	35.0	41.0
16	38.927329305525355	41.0	37.0	41.0	35.0	41.0
17	38.95809258738378	41.0	37.0	41.0	35.0	41.0
18	38.943479787579406	41.0	37.0	41.0	35.0	41.0
19	38.887229407374974	41.0	37.0	41.0	35.0	41.0
20	38.823947729644985	41.0	36.0	41.0	35.0	41.0
21	38.77430492606417	41.0	36.0	41.0	35.0	41.0
22	38.72937575400939	41.0	36.0	41.0	35.0	41.0
23	38.69476706191866	41.0	35.0	41.0	35.0	41.0
24	38.63975532000113	41.0	35.0	41.0	35.0	41.0
25	38.57997634149676	40.0	35.0	41.0	35.0	41.0
26	38.50086882359779	40.0	35.0	41.0	35.0	41.0
27	38.452788095580836	40.0	36.0	41.0	35.0	41.0
28	38.481302675253914	40.0	36.0	41.0	35.0	41.0
29	38.44424892985627	40.0	36.0	41.0	35.0	41.0
30	38.3990327599198	40.0	35.0	41.0	35.0	41.0
31	38.34914361398092	40.0	35.0	41.0	35.0	41.0
32	38.26999905438264	40.0	35.0	41.0	35.0	41.0
33	38.1633722642597	40.0	35.0	41.0	35.0	41.0
34	38.065195197709315	40.0	35.0	41.0	35.0	41.0
35	38.01621221821958	40.0	35.0	41.0	35.0	41.0
36	37.953921029506276	40.0	35.0	41.0	35.0	41.0
37	37.877925917805975	40.0	35.0	41.0	35.0	41.0
38	37.80723830942889	40.0	35.0	41.0	35.0	41.0
39	37.710170686945844	40.0	35.0	41.0	34.0	41.0
40	37.60030109179656	40.0	35.0	41.0	34.0	41.0
41	37.55099527733391	40.0	35.0	41.0	34.0	41.0
42	37.49006740384667	40.0	35.0	41.0	34.0	41.0
43	37.503618642755995	39.0	35.0	41.0	34.0	41.0
44	37.455534903218776	39.0	35.0	41.0	34.0	41.0
45	37.427591608940844	39.0	35.0	41.0	34.0	41.0
46	37.39147324173895	39.0	35.0	41.0	34.0	41.0
47	37.28348705136629	39.0	35.0	41.0	34.0	41.0
48	37.23633567850455	39.0	35.0	41.0	34.0	41.0
49	37.18918400449078	39.0	35.0	41.0	34.0	41.0
50	37.10764679203826	39.0	35.0	41.0	34.0	41.0
51	37.02497513990017	38.0	35.0	41.0	34.0	41.0
52	36.756180994777424	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	9.0
12	4.0
13	7.0
14	22.0
15	37.0
16	93.0
17	156.0
18	267.0
19	492.0
20	940.0
21	1533.0
22	2459.0
23	3957.0
24	6425.0
25	10430.0
26	14318.0
27	17063.0
28	18392.0
29	20664.0
30	24364.0
31	29838.0
32	38818.0
33	54939.0
34	180536.0
35	490111.0
36	147939.0
37	229777.0
38	375825.0
39	1635442.0
40	15721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.02983392670321	20.78349518247102	22.95618659620512	19.23048429462064
2	30.75463879524734	22.872044719871397	24.423218580357297	21.950097904523965
3	30.284239329129655	23.93995992268825	23.71174691665497	22.064053831527126
4	27.90013919246686	25.70486137671047	21.922030535610926	24.472968895211743
5	25.996406654014265	34.794141508928256	20.650596792971836	18.558855044085647
6	84.70795179881118	3.244009634455647	9.093134878162925	2.9549036885702566
7	86.55464614335679	3.1848031459545343	6.260589258148119	3.9999614525405485
8	75.40509464907056	5.909144842681193	11.781007064424248	6.904753443824005
9	49.601997481164446	20.056363613366575	15.557694404173727	14.783944501295254
10	38.99509182426454	21.924801134258995	23.86403949669064	15.216067544785824
11	35.283664128758154	19.99173638838011	25.020493395434894	19.704106087426844
12	24.52311070770124	25.27882160416457	29.997783521081544	20.200284167052644
13	19.49757602733497	27.77446845161481	31.770966655845275	20.956988865204956
14	18.346181482643704	24.671759348210646	36.66706619502244	20.31499297412321
15	21.139637569558587	20.28993712547981	37.706582761696595	20.863842543265008
16	22.22035173352141	22.54948078379031	27.00921103589672	28.220956446791558
17	22.594894509456477	23.311274951198314	28.748394106816217	25.345436432528995
18	24.847180403917143	26.640932222122508	27.54977892429701	20.96210844966334
19	27.255854545980192	22.907731235066624	25.230938431877302	24.605475787075882
20	24.72933961576615	27.109494660875715	27.05881077473768	21.10235494862045
21	23.049393148550465	22.806423693195953	28.316361408933737	25.827821749319845
22	21.75928798023961	25.736151072312026	26.50682922451546	25.997731722932908
23	22.963323899244166	22.72183008882178	31.845863164951204	22.46898284698285
24	24.57063249755615	21.158580032054623	28.71379173891806	25.556995731471172
25	20.117316783624076	27.348308218258126	28.06649557216175	24.467879425956053
26	19.36347302972792	26.463945175875796	29.008709918923852	25.16387187547243
27	22.95609625059703	24.49344723304529	30.512331874352146	22.038124642005528
28	20.042811772153197	26.060070192514445	28.686326674058943	25.210791361273415
29	22.666508461468503	24.018620832131234	30.50715205948837	22.807718646911898
30	26.269732233686742	23.33160271301838	24.55409925127583	25.844565802019044
31	26.352127428264083	24.75680468062526	27.79416379417825	21.096904096932406
32	24.894069774515433	25.625959545645916	26.208417680996888	23.27155299884177
33	22.73995944084501	23.05237455361741	29.860066699150934	24.347599306386652
34	21.685294927214567	22.98018841275415	31.45087216638529	23.883644493645996
35	25.765392934130222	24.13709403953885	27.686923557376385	22.41058946895454
36	25.550340271675264	25.07828446940928	26.91525160348397	22.456123655431487
37	23.153230367447634	25.126438678520813	29.04662495911861	22.67370599491294
38	22.80377355535867	23.70241120381909	27.932392574554704	25.561422666267543
39	27.31936750846689	22.059024592676828	28.566709088948866	22.05489880990742
40	20.54883752306072	20.776568685850854	32.67677172254743	25.997822068540998
41	21.544054626568474	24.538047848238655	26.9935210152919	26.924376509900977
42	22.28485849769709	21.7902464086115	29.615109640418456	26.309785453272948
43	23.906200780465593	24.893256664042628	28.20981382179389	22.99072873369789
44	21.825812462995945	24.76309875798881	27.255432933142444	26.1556558458728
45	22.014815475118517	23.5820407386416	27.808137248229375	26.59500653801051
46	24.347328269562386	22.0935064997642	29.974414123789145	23.584751106884276
47	22.00469676701253	21.666774077556283	32.466417031713114	23.86211212371807
48	21.9606984558731	21.34011447390849	28.91014286049855	27.78904420971986
49	21.16427180536424	22.924264481346945	31.606868916352614	24.3045947969362
50	20.919947165888388	23.32615186133033	30.625595151693286	25.128305821087988
51	21.913538048450544	20.871853187182246	29.091135228703884	28.12347353566333
52	22.381889680784877	19.37476623073907	33.04023210389022	25.203111984585835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	688.0
1	1407.0
2	2126.0
3	8030.0
4	13934.0
5	9109.5
6	4285.0
7	4465.5
8	4646.0
9	4783.0
10	4920.0
11	4778.0
12	4636.0
13	4702.0
14	4750.0
15	4732.0
16	4657.0
17	4582.0
18	4830.0
19	5078.0
20	5718.5
21	6359.0
22	7355.0
23	8351.0
24	9192.5
25	10034.0
26	11914.5
27	13795.0
28	18259.5
29	22724.0
30	24849.0
31	26974.0
32	31589.0
33	36204.0
34	42066.5
35	47929.0
36	50746.5
37	53564.0
38	61184.0
39	81748.5
40	94693.0
41	121083.5
42	147474.0
43	191178.0
44	234882.0
45	284246.0
46	333610.0
47	350356.5
48	367103.0
49	344704.5
50	322306.0
51	283191.5
52	244077.0
53	221313.5
54	198550.0
55	183977.5
56	169405.0
57	160415.5
58	151426.0
59	155619.0
60	159812.0
61	153465.5
62	147119.0
63	138065.5
64	107334.0
65	85656.0
66	72509.5
67	59363.0
68	51392.0
69	43421.0
70	37027.0
71	30633.0
72	25994.0
73	21355.0
74	17572.0
75	13789.0
76	11372.0
77	8955.0
78	6765.5
79	4576.0
80	3670.5
81	2765.0
82	1955.5
83	1146.0
84	648.5
85	151.0
86	123.5
87	96.0
88	58.0
89	25.0
90	30.0
91	24.5
92	19.0
93	12.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3320582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.697468576215392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.65819701643552	14.003534066710642
2	13.077518287153175	5.4134304760647
3	5.5218971347791825	3.4286787728455783
4	2.863730103431279	2.370878553061238
5	1.5763113852807054	1.6312827681588977
6	1.052285859908891	1.306779211119604
7	0.7096686034301455	1.028184053331547
8	0.5129933386616515	0.8494130805405882
9	0.3836930462759812	0.7147327291388531
>10	4.226633701565553	22.042694732192572
>50	2.0939631558514624	30.012296407527316
>100	0.3017215813001068	9.74952982458542
>500	0.012158241314314217	1.7180064561675843
>1k	0.00805666593117207	3.1654795741478683
>5k	5.859393404488778E-4	0.9531578040941765
>10k+	5.859393404488778E-4	1.6119214903133467
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	16018	0.4823853167908517	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	13496	0.4064347755905441	TruSeq Adapter, Index 20 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	12350	0.37192275330047564	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	11302	0.3403620208746539	TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9792	0.2948880648030978	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8823	0.2657064333902912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7801	0.2349286962345757	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	5022	0.15123854794129463	TruSeq Adapter, Index 20 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4210	0.12678500335182205	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	3861	0.11627479761078029	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	3824	0.11516053511101366	TruSeq Adapter, Index 22 (95% over 23bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3765	0.11338373815192639	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3619	0.10898691855825275	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3563	0.10730046720725463	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3529	0.1062765503155772	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	3380	0.10178938511381438	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41531876038598053	0.0
2	0.0	0.0	0.0	1.447155950372555	0.0
3	0.0	0.0	0.0	2.2299102988572486	0.0
4	0.0	0.0	0.0	2.7321415342250246	0.0
5	0.0	0.0	0.0	5.366920618132604	0.0
6	0.0	0.0	0.0	5.773536084939327	0.0
7	0.0	0.0	0.0	6.964983849216794	0.0
8	0.0	0.0	0.0	8.53350406645582	0.0
9	0.0	0.0	0.0	9.581302313871484	0.0
10	3.011520269639479E-5	0.0	0.0	10.617415862640947	0.0
11	3.011520269639479E-5	0.0	0.0	11.5851076708842	0.0
12	3.011520269639479E-5	0.0	0.0	12.46528470009173	0.0
13	3.011520269639479E-5	0.0	0.0	12.89599232905557	0.0
14	9.034560808918437E-5	0.0	0.0	13.183863551630408	0.0
15	9.034560808918437E-5	0.0	0.0	14.06885901326936	0.0
16	9.034560808918437E-5	0.0	0.0	15.000472808682334	0.0
17	9.034560808918437E-5	0.0	0.0	16.042790089207255	0.0
18	9.034560808918437E-5	0.0	0.0	16.721918025213654	0.0
19	9.034560808918437E-5	0.0	0.0	17.39613718318054	0.0
20	9.034560808918437E-5	0.0	0.0	18.253818155973864	0.0
21	1.8069121617836874E-4	0.0	0.0	19.015461747368384	0.0
22	1.8069121617836874E-4	0.0	0.0	19.817038097538322	0.0
23	1.8069121617836874E-4	0.0	0.0	20.432984338287685	0.0
24	2.1080641887476353E-4	0.0	0.0	20.990988929049184	0.0
25	2.1080641887476353E-4	0.0	0.0	21.545861538730257	0.0
26	2.1080641887476353E-4	0.0	0.0	22.08239398996923	0.0
27	2.1080641887476353E-4	0.0	0.0	22.60203181249552	0.0
28	2.1080641887476353E-4	0.0	0.0	23.15711522859547	0.0
29	2.710368242675531E-4	0.0	0.0	23.738428986243978	0.0
30	2.710368242675531E-4	0.0	0.0	24.55951998776118	0.0
31	2.710368242675531E-4	0.0	0.0	25.127372249804402	0.0
32	2.710368242675531E-4	0.0	0.0	25.663362627394836	0.0
33	2.710368242675531E-4	0.0	0.0	26.275393891793666	0.0
34	2.710368242675531E-4	0.0	0.0	26.98864235245508	0.0
35	2.710368242675531E-4	0.0	0.0	27.546707173621975	0.0
36	2.710368242675531E-4	0.0	0.0	28.132839363701905	0.0
37	2.710368242675531E-4	0.0	0.0	28.737131021007762	0.0
38	2.710368242675531E-4	0.0	0.0	29.45646275261385	0.0
39	2.710368242675531E-4	0.0	0.0	30.050515241002934	0.0
40	2.710368242675531E-4	0.0	0.0	30.61478379392528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAAT	20	6.313186E-4	46.0	18
CTAACGG	260	0.0	43.34615	1
AATACGG	140	0.0	41.07143	1
CGTTTTT	5245	0.0	40.9571	1
CGACGGT	355	0.0	40.816902	27
TAGTAGG	530	0.0	40.792454	1
CGGTCTA	340	0.0	40.588234	30
TCTAACG	40	2.9128023E-7	40.25	46
ATCAACG	3580	0.0	40.089386	14
TCAACGC	3600	0.0	39.802776	15
CACTTAC	3190	0.0	39.79937	36
CCGCACT	3215	0.0	39.70451	33
CACGACG	365	0.0	39.69863	25
CGCACTT	3215	0.0	39.56143	34
CATACGA	640	0.0	39.53125	17
CCCGCAC	3250	0.0	39.41846	32
TACGGGA	695	0.0	39.381294	3
AACGCAA	3605	0.0	39.364773	17
CTTAATC	4020	0.0	39.363182	10
CGCAAGC	3615	0.0	39.25588	19
>>END_MODULE