FastQCFastQC Report
Thu 26 May 2016
SRR1544879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544879_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2350950
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT178410.7588847061826071TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT150530.6402943490929198TruSeq Adapter, Index 20 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA133990.5699398115655374No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT120230.5114102809502541TruSeq Adapter, Index 22 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTTG82700.3517726876369128TruSeq Adapter, Index 22 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC75790.3223803143410111No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC63160.2686573512835237No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG61160.26015015206618597No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC43240.18392564707884046TruSeq Adapter, Index 20 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC38670.1644866968672239TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT37570.15980773729768816TruSeq Adapter, Index 22 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC34620.1472596184521151TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.14704693847168165No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT33180.141134435015632No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27910.11871796507794723No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC27760.11807992513664688TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC25700.10931750994278909TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT25490.10842425402496864TruSeq Adapter, Index 20 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT24950.10612731023628746No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT24100.10251175056891894No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23990.10204385461196537No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG23940.10183117463153192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG301.8622395E-646.041
CCGTAGG700.042.7142871
GTCGGCG21950.042.437363
TCGGCGT21900.041.904114
CTAACGG1850.041.0270271
TAGTAGG3700.041.0270271
GTTACGG2300.041.01
ATAGCGG2750.040.981821
CACTTAC23250.040.9548436
CATGCGG4550.040.9450571
GTCCCCC22550.040.9002239
CCCAACT21950.040.76082213
CGGTCGG23250.040.6580661
CGCACTT23400.040.59401734
CACGACG2100.040.52380825
CCGCACT23450.040.5074633
ATCAACG27600.040.33333214
GCGTCCC22800.040.149127
CGTTTTT30450.040.108371
CCCGCAC24050.040.07068632
Produced by FastQC (version 0.11.5)