##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544879_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2350950
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.847891703353966	34.0	33.0	34.0	31.0	34.0
2	33.21519640996193	34.0	33.0	34.0	31.0	34.0
3	33.31750483846955	34.0	34.0	34.0	31.0	34.0
4	36.54195665581999	37.0	37.0	37.0	35.0	37.0
5	36.53563665752143	37.0	37.0	37.0	35.0	37.0
6	36.450370275845934	37.0	37.0	37.0	35.0	37.0
7	36.33573193815266	37.0	37.0	37.0	35.0	37.0
8	36.37803058338119	37.0	37.0	37.0	35.0	37.0
9	38.24597418065038	39.0	39.0	39.0	37.0	39.0
10	38.14708181798847	39.0	39.0	39.0	35.0	39.0
11	37.73123715944618	39.0	37.0	39.0	35.0	39.0
12	37.69958612475808	39.0	37.0	39.0	35.0	39.0
13	37.64039643548353	39.0	37.0	39.0	35.0	39.0
14	38.99789234139391	41.0	38.0	41.0	35.0	41.0
15	39.06454369510198	41.0	38.0	41.0	35.0	41.0
16	39.06286565005636	41.0	38.0	41.0	35.0	41.0
17	39.103731257576726	41.0	38.0	41.0	35.0	41.0
18	39.10394904187669	41.0	38.0	41.0	35.0	41.0
19	39.06316552882877	41.0	38.0	41.0	35.0	41.0
20	39.01019630362194	41.0	38.0	41.0	35.0	41.0
21	38.96339011888811	41.0	37.0	41.0	35.0	41.0
22	38.92894361853719	41.0	37.0	41.0	35.0	41.0
23	38.904151938578025	41.0	37.0	41.0	35.0	41.0
24	38.82832684659393	41.0	37.0	41.0	35.0	41.0
25	38.766204300389205	41.0	36.0	41.0	35.0	41.0
26	38.68142793338863	41.0	36.0	41.0	35.0	41.0
27	38.65018949786256	40.0	36.0	41.0	35.0	41.0
28	38.71166166868713	41.0	36.0	41.0	35.0	41.0
29	38.71041451328186	41.0	36.0	41.0	35.0	41.0
30	38.68940045513516	41.0	36.0	41.0	35.0	41.0
31	38.661792892235056	41.0	36.0	41.0	35.0	41.0
32	38.605275314234675	41.0	36.0	41.0	35.0	41.0
33	38.53280205874221	40.0	36.0	41.0	35.0	41.0
34	38.4126880622727	40.0	36.0	41.0	35.0	41.0
35	38.37275271698675	40.0	36.0	41.0	35.0	41.0
36	38.33315510750973	40.0	35.0	41.0	35.0	41.0
37	38.27445926114975	40.0	35.0	41.0	35.0	41.0
38	38.19509857717093	40.0	35.0	41.0	35.0	41.0
39	38.159681405389314	40.0	35.0	41.0	35.0	41.0
40	38.09233416278526	40.0	35.0	41.0	35.0	41.0
41	38.054315489482974	40.0	35.0	41.0	35.0	41.0
42	38.01165188540803	40.0	35.0	41.0	35.0	41.0
43	37.952686786192814	40.0	35.0	41.0	35.0	41.0
44	37.81667623726579	40.0	35.0	41.0	35.0	41.0
45	37.79474170016376	40.0	35.0	41.0	35.0	41.0
46	37.74820944724473	40.0	35.0	41.0	35.0	41.0
47	37.66104723622365	39.0	35.0	41.0	35.0	41.0
48	37.584462876709416	39.0	35.0	41.0	35.0	41.0
49	37.56348454879942	39.0	35.0	41.0	35.0	41.0
50	37.49234692358408	39.0	35.0	41.0	35.0	41.0
51	37.4146923584083	39.0	35.0	41.0	35.0	41.0
52	36.98832216763436	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	7.0
13	15.0
14	21.0
15	24.0
16	45.0
17	113.0
18	176.0
19	307.0
20	580.0
21	952.0
22	1487.0
23	2316.0
24	3744.0
25	5770.0
26	7989.0
27	9343.0
28	9665.0
29	10908.0
30	13015.0
31	16799.0
32	22095.0
33	32547.0
34	113705.0
35	329765.0
36	97061.0
37	157793.0
38	266415.0
39	1238808.0
40	9481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.062485378251345	20.604989472340968	21.911907952104468	21.42061719730322
2	30.45615602203365	22.998702652119356	24.17529083987324	22.369850485973757
3	30.464833365235332	23.645972904570492	23.595312533231247	22.293881196962932
4	27.755673238478064	24.952976456326166	22.52195920797975	24.76939109721602
5	25.637508241349245	33.64329313681703	20.88436589463834	19.83483272719539
6	82.09319636742593	3.3743805695569873	10.343860992364789	4.1885620706522895
7	84.25972479210532	3.232863310576575	7.421893277185819	5.085518620132287
8	71.57783023883961	6.113230821582764	13.647589272421786	8.66134966715583
9	48.10774367808758	19.238775814032625	16.553946277036943	16.099534230842853
10	37.128990408132886	21.108955953976054	26.242114889725432	15.519938748165634
11	33.21733767200493	19.70492779514664	25.470086560752037	21.607647972096387
12	23.48340032752717	23.969501690805846	31.24634722133606	21.30075076033093
13	18.886832982411367	26.781513856100723	32.569216699632065	21.762436461855845
14	17.79476381888173	24.682915417171785	35.552053425211085	21.970267338735404
15	20.160615921223336	20.21837980390906	37.55209596120717	22.068908313660433
16	21.10950892192518	23.82687849592718	26.954805504157893	28.108807077989752
17	21.316786830855612	23.797741338607796	28.743997107552268	26.141474722984327
18	23.380335609009123	26.93545162593845	28.065633041961764	21.618579723090665
19	25.675875709819433	22.607711776090518	25.793487738999126	25.922924775090923
20	23.350305195771924	27.674812309917268	27.661881367106915	21.313001127203897
21	22.18609498287926	22.643186796826814	28.54935238945958	26.621365830834343
22	20.852293753588974	25.54814011357111	26.544801037878308	27.054765094961613
23	21.734064952466024	22.622726982709118	32.430719496373804	23.21248856845105
24	24.616218975307856	21.44503285905698	28.070567217507815	25.86818094812735
25	19.714923754227016	27.01018737106276	27.793147451030435	25.48174142367979
26	18.74616644335269	25.73232097662647	29.204704481167187	26.316808098853656
27	23.621684850804993	23.715476722176142	29.94589421297773	22.71694421404113
28	20.12152534081967	25.299517216444418	28.306854675769372	26.272102766966544
29	22.53497522278228	23.409302622344157	30.849996809800295	23.20572534507327
30	25.639124609200536	23.313809311129543	24.21578510814777	26.831280971522148
31	25.612539611646355	23.59990642080861	28.78695846359982	22.000595503945213
32	23.824624088134584	25.589442565771286	26.87892128713924	23.70701205895489
33	23.061400710351133	22.122843956698357	29.99906420808609	24.816691124864416
34	21.74503923945639	21.840064654714052	32.600097832791	23.81479827303856
35	25.143239966821923	23.18862587464642	29.120866032880325	22.547268125651332
36	26.56441013207427	24.057593738701378	26.880027223037494	22.49796890618686
37	23.05604117484421	24.128926604138755	30.670537442310554	22.14449477870648
38	22.71388162232289	22.719709053786765	28.114847189434062	26.451562134456285
39	28.08026542461558	20.994831876475466	29.09168633956486	21.833216359344096
40	20.64816350836896	19.5184499883026	33.653842063846525	26.179544439481912
41	21.64741912843744	23.80246283417342	27.415810629745422	27.13430740764372
42	21.81079988940641	20.463599821348815	30.51711010442587	27.2084901848189
43	24.76271294583041	23.822837576298944	28.10238414258066	23.31206533528999
44	21.935813181905186	23.240817541844784	28.128586316170058	26.694782960079966
45	21.61236946766201	21.986941449201385	28.53633637465705	27.86435270847955
46	24.68372360109743	20.490737786852122	30.433867160084223	24.391671451966225
47	21.108275378038666	20.675003721899657	33.98455943342053	24.232161466641145
48	21.69671834790191	19.798847274506052	29.081945596461008	29.422488781131033
49	21.247495693230395	21.055615814883343	32.58397669027415	25.112911801612114
50	20.957527807907443	21.376592441353495	31.7542695506072	25.91161020013186
51	21.937259405772135	19.1634445649631	29.54392904995853	29.355366979306236
52	21.83942661477275	18.452200174397586	34.76900827325124	24.939364937578425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	745.0
2	1051.0
3	3891.5
4	6732.0
5	4627.5
6	2523.0
7	2566.5
8	2610.0
9	2674.5
10	2739.0
11	2745.5
12	2752.0
13	2808.5
14	2847.0
15	2829.0
16	2841.0
17	2853.0
18	2952.0
19	3051.0
20	3505.5
21	3960.0
22	4608.0
23	5256.0
24	5712.0
25	6168.0
26	7294.5
27	8421.0
28	11266.0
29	14111.0
30	15233.5
31	16356.0
32	19092.5
33	21829.0
34	25474.5
35	29120.0
36	31306.5
37	33493.0
38	38637.0
39	52322.5
40	60864.0
41	79324.0
42	97784.0
43	131474.5
44	165165.0
45	213556.5
46	261948.0
47	277143.0
48	292338.0
49	269638.0
50	246938.0
51	212869.5
52	178801.0
53	159867.5
54	140934.0
55	127999.5
56	115065.0
57	107790.0
58	100515.0
59	102276.5
60	104038.0
61	100107.0
62	96176.0
63	95701.5
64	76367.0
65	57507.0
66	48481.5
67	39456.0
68	34286.0
69	29116.0
70	24805.0
71	20494.0
72	17338.0
73	14182.0
74	11783.5
75	9385.0
76	7699.5
77	6014.0
78	4667.5
79	3321.0
80	2602.0
81	1883.0
82	1294.5
83	706.0
84	396.0
85	86.0
86	64.0
87	42.0
88	22.5
89	9.0
90	15.0
91	8.0
92	1.0
93	4.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2350950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.897824823089536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.3207220320978	16.32716646910067
2	12.958069001282823	6.193393260763274
3	5.198953353764772	3.7273102953505304
4	2.5127822786892833	2.40200122858721
5	1.4577468862609289	1.7418489862133952
6	0.9523607503751194	1.3655610228510424
7	0.6754500042940295	1.1299250115561654
8	0.5081211372770967	0.971439194204567
9	0.4007180516934137	0.8618660822537042
>10	6.111468452006264	38.70445652590211
>50	0.7331957803537575	10.90695499515215
>100	0.14897711162352206	6.304463382604736
>500	0.011789338154688668	1.9256470544274689
>1k	0.008216811441146646	3.7413303134170044
>5k	7.14505342708404E-4	1.2072539203339225
>10k+	7.14505342708404E-4	2.4893822572820277
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	17841	0.7588847061826071	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	15053	0.6402943490929198	TruSeq Adapter, Index 20 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	13399	0.5699398115655374	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	12023	0.5114102809502541	TruSeq Adapter, Index 22 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTTG	8270	0.3517726876369128	TruSeq Adapter, Index 22 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	7579	0.3223803143410111	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6316	0.2686573512835237	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6116	0.26015015206618597	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	4324	0.18392564707884046	TruSeq Adapter, Index 20 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	3867	0.1644866968672239	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	3757	0.15980773729768816	TruSeq Adapter, Index 22 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	3462	0.1472596184521151	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3457	0.14704693847168165	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3318	0.141134435015632	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2791	0.11871796507794723	No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	2776	0.11807992513664688	TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	2570	0.10931750994278909	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	2549	0.10842425402496864	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	2495	0.10612731023628746	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	2410	0.10251175056891894	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2399	0.10204385461196537	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	2394	0.10183117463153192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6506731321380719	0.0
2	0.0	0.0	0.0	2.2153172121908167	0.0
3	0.0	0.0	0.0	3.3048767519513387	0.0
4	0.0	0.0	0.0	4.0282864373976475	0.0
5	0.0	0.0	0.0	7.590718645653885	0.0
6	0.0	0.0	0.0	8.14989685020949	0.0
7	0.0	0.0	0.0	9.77868521236096	0.0
8	0.0	0.0	0.0	11.805312745911227	0.0
9	0.0	0.0	0.0	13.169144388438717	0.0
10	0.0	0.0	0.0	14.52008762415194	0.0
11	0.0	0.0	0.0	15.75312107871286	0.0
12	4.253599608668836E-5	0.0	0.0	16.862842680618474	0.0
13	8.507199217337671E-5	0.0	0.0	17.41313086199196	0.0
14	8.507199217337671E-5	0.0	0.0	17.768689253280588	0.0
15	8.507199217337671E-5	0.0	0.0	18.85042216976116	0.0
16	8.507199217337671E-5	0.0	0.0	19.95261490035943	0.0
17	8.507199217337671E-5	0.0	0.0	21.141453454986284	0.0
18	1.2760798826006508E-4	0.0	0.0	21.93759969374083	0.0
19	1.2760798826006508E-4	0.0	0.0	22.733533252514942	0.0
20	1.7014398434675343E-4	0.0	0.0	23.689997660520216	0.0
21	1.7014398434675343E-4	0.0	0.0	24.565473531976433	0.0
22	2.5521597652013015E-4	0.0	0.0	25.473617048427233	0.0
23	2.5521597652013015E-4	0.0	0.0	26.14645143452647	0.0
24	2.5521597652013015E-4	0.0	0.0	26.769944065165145	0.0
25	2.5521597652013015E-4	0.0	0.0	27.370509793913097	0.0
26	2.5521597652013015E-4	0.0	0.0	27.961972819498502	0.0
27	2.5521597652013015E-4	0.0	0.0	28.51519598460197	0.0
28	2.5521597652013015E-4	0.0	0.0	29.1084880580191	0.0
29	2.5521597652013015E-4	0.0	0.0	29.727514409068675	0.0
30	2.5521597652013015E-4	0.0	0.0	30.580488738595037	0.0
31	2.5521597652013015E-4	0.0	0.0	31.172121908164783	0.0
32	2.5521597652013015E-4	0.0	0.0	31.734064952466024	0.0
33	2.5521597652013015E-4	0.0	0.0	32.3630021906038	0.0
34	2.5521597652013015E-4	0.0	0.0	33.075139837087136	0.0
35	2.5521597652013015E-4	0.0	0.0	33.637550777345325	0.0
36	2.5521597652013015E-4	0.0	0.0	34.232671898594184	0.0
37	2.5521597652013015E-4	0.0	0.0	34.8531870095068	0.0
38	2.5521597652013015E-4	0.0	0.0	35.587230693974774	0.0
39	2.977519726068185E-4	0.0	0.0	36.18235181522363	0.0
40	2.977519726068185E-4	0.0	0.0	36.7534826346796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATACG	30	1.8622395E-6	46.0	41
CCGTAGG	70	0.0	42.714287	1
GTCGGCG	2195	0.0	42.43736	3
TCGGCGT	2190	0.0	41.90411	4
CTAACGG	185	0.0	41.027027	1
TAGTAGG	370	0.0	41.027027	1
GTTACGG	230	0.0	41.0	1
ATAGCGG	275	0.0	40.98182	1
CACTTAC	2325	0.0	40.95484	36
CATGCGG	455	0.0	40.945057	1
GTCCCCC	2255	0.0	40.900223	9
CCCAACT	2195	0.0	40.760822	13
CGGTCGG	2325	0.0	40.658066	1
CGCACTT	2340	0.0	40.594017	34
CACGACG	210	0.0	40.523808	25
CCGCACT	2345	0.0	40.50746	33
ATCAACG	2760	0.0	40.333332	14
GCGTCCC	2280	0.0	40.14912	7
CGTTTTT	3045	0.0	40.10837	1
CCCGCAC	2405	0.0	40.070686	32
>>END_MODULE